The Family Methylococcaceae

Bowman, John P.

doi:10.1007/978-3-642-38922-1_237

Cited by 27 publications

(21 citation statements)

References 199 publications

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“…The vast majority of these bacteria use methane as carbon and energy source (Bowman, 2014). Interestingly, nodes from Methylococcales were well represented in several samples from the MAR and L o'ihi, including veil and non-veil mats.…”

Section: Implications For An Iron Mat Microbiomementioning

confidence: 99%

“…Interestingly, nodes from Methylococcales were well represented in several samples from the MAR and L o'ihi, including veil and non-veil mats. The vast majority of these bacteria use methane as carbon and energy source (Bowman, 2014). Unfortunately, we did not measure methane in vent fluids in areas of community profiling.…”

Section: Implications For An Iron Mat Microbiomementioning

confidence: 99%

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Bringing microbial diversity into focus: high‐resolution analysis of iron mats from the Lō‘ihi Seamount

Scott

Glazer

Emerson

2017

Environmental Microbiology

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Thirty kilometers south of the island of Hawai'i lies the Lō'ihi Seamount, an active submarine volcano that hosts a network of low-temperature hydrothermal vents enriched in ferrous iron that supports extensive microbial mats. These mats, which can be a half a meter deep, are composed of ferric iron bound to organic polymers - the metabolic byproduct of iron-oxidizing Zetaproteobacteria. Though the role of Zetaproteobacteria in mat formation is well established, we have a limited understanding of how differences in diversity are related to mat morphology. We used Minimum Entropy Decomposition and ZetaOtu classification to demonstrate cryptic diversity between closely related Zetaproteobacteria while showing habitat and geographic specificity. Veiled mats, common structures at Lō'ihi, exhibit distinct community composition and contain diversity not detected in other mat types, including specific Zetaproteobacteria and an unclassified Gammaproteobacteria. Our analyses also indicate that diversity can change dramatically across small spatial transects from points of active venting, yet we found comparatively few differences between major sampling sites. This study provides a better picture of the microbiome responsible for iron mat production at Lō'ihi and has broad implications for our understanding of these globally distributed communities.

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Section: Implications For An Iron Mat Microbiomementioning

confidence: 99%

Section: Implications For An Iron Mat Microbiomementioning

confidence: 99%

Bringing microbial diversity into focus: high‐resolution analysis of iron mats from the Lō‘ihi Seamount

Scott

Glazer

Emerson

2017

Environmental Microbiology

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“…The bacterium Methylococcus capsulatus (Bath), an obligate aerobic methanotroph, is a model organism for 63 studies of the genetics, physiology, and geomicrobiology of aerobic methane oxidation in sediments and water 64 columns (Whittenbury et al, 1970;Bowman, 2014). This organism uses the enzymes soluble methane 65 monooxygenase (sMMO) and particulate methane monooxygenase (pMMO) to oxidize methane to methanol, 66 which is further oxidized to CO 2 as an end product (Hanson and Hanson, 1996).…”

mentioning

confidence: 99%

Fractionation of the methane isotopologues 13CH4, 12CH3D, and 13CH3D during aerobic oxidation of methane by Methylococcus capsulatus (Bath)

Wang

Welander

Ono

2016

Geochimica et Cosmochimica Acta

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“…The bacterium Methylococcus capsulatus (Bath), an obligate aerobic methanotroph, is a model organism for studies of the genetics, physiology, and geomicrobiology of aerobic methane oxidation in sediments and water columns (Whittenbury et al, 1970;Bowman, 2014). This organism uses the enzymes soluble methane monooxygenase (sMMO) and particulate methane monooxygenase (pMMO) to oxidize methane to methanol, which is further oxidized to CO 2 as an end product (Hanson and Hanson, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, activity of methanotrophic bacteria with high affinity for atmospheric methane in Arctic soils has been reported (Lau et al, 2015). Thus, understanding the magnitude and dynamics of methanotrophic sinks is important for global methane cycle budgets and constraining inputs to climate simulations.The bacterium Methylococcus capsulatus (Bath), an obligate aerobic methanotroph, is a model organism for studies of the genetics, physiology, and geomicrobiology of aerobic methane oxidation in sediments and water columns (Whittenbury et al, 1970;Bowman, 2014). This organism uses the enzymes soluble methane monooxygenase (sMMO) and particulate methane monooxygenase (pMMO) to oxidize methane to methanol, which is further oxidized to CO 2 as an end product (Hanson and Hanson, 1996).…”

mentioning

confidence: 99%

The geochemistry of methane isotopologues

Wang¹

2017

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This thesis documents the origin, distribution, and fate of methane and several of its isotopic forms on Earth. Using observational, experimental, and theoretical approaches, I illustrate how the relative abundances of 12 CH 4 , 13 CH 4 , 12 CH 3 D, and 13 CH 3 D record the formation, transport, and breakdown of methane in selected settings.Chapter 2 reports precise determinations of 13 CH 3 D, a "clumped" isotopologue of methane, in samples collected from various settings representing many of the major sources and reservoirs of methane on Earth. The results show that the information encoded by the abundance of 13 CH 3 D enables differentiation of methane generated by microbial, thermogenic, and abiogenic processes. A strong correlation between clumped-and hydrogen-isotope signatures in microbial methane is identified and quantitatively linked to the availability of H 2 and the reversibility of microbially-mediated methanogenesis in the environment. Determination of 13 CH 3 D in combination with hydrogen-isotope ratios of methane and water provides a sensitive indicator of the extent of C-H bond equilibration, enables fingerprinting of methane-generating mechanisms, and in some cases, supplies direct constraints for locating the waters from which migrated gases were sourced. Chapter 3 applies this concept to constrain the origin of methane in hydrothermal fluids from sediment-poor vent fields hosted in mafic and ultramafic rocks on slow-and ultraslow-spreading mid-ocean ridges. The data support a hypogene model whereby methane forms abiotically within plutonic rocks of the oceanic crust at temperatures above ca. 300• C during respeciation of magmatic volatiles, and is subsequently extracted during active, convective hydrothermal circulation. Chapter 4 presents the results of culture experiments in which methane is oxidized in the presence of O 2 by the bacterium Methylococcus capsulatus strain Bath. The results show that the clumped isotopologue abundances of partially-oxidized methane can be predicted from knowledge of 13 C/ 12 C and D/H isotope fractionation factors alone.: Shuhei Ono, Ph.D. Acknowledgments I fear that this section will not do justice to the people I have not the space to thank individually. Alas, here goes. To those whose names are not specifically listed here, thank you too. I wish first to thank Shuhei Ono. The work this thesis represents could not have happened without his bold vision and the license to explore and question that working in his lab afforded. His unbridled optimism, breadth of scientific curiosity, personal integrity, and accessibility to his personnel are traits I one day hope to emulate. I thank him for his nonjudgmental yet appropriately measured support of many of my ideas-even those that led nowhere-, for believing in me even when I found it difficult to do so myself, and for teaching me to change pump oil, build vacuum lines, and drink Icelandic vodka.I also wish to thank my thesis committee members Jeff Seewald, Roger Summons, and John Pohlman, for their in...

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The Family Methylococcaceae

Cited by 27 publications

References 199 publications

Bringing microbial diversity into focus: high‐resolution analysis of iron mats from the Lō‘ihi Seamount

Bringing microbial diversity into focus: high‐resolution analysis of iron mats from the Lō‘ihi Seamount

Fractionation of the methane isotopologues 13CH4, 12CH3D, and 13CH3D during aerobic oxidation of methane by Methylococcus capsulatus (Bath)

The geochemistry of methane isotopologues

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