The fate of antibiotic resistance marker genes in transgenic plant feed material fed to chickens

Chambers, Philip A.; Duggan, Paula S.; Forbes, J. M.

doi:10.1093/jac/49.1.161

Cited by 47 publications

(31 citation statements)

References 7 publications

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“…The high level of degradation of ubiquitous plant chloroplast DNA in the last section of cattle GIT (jejunum and colon) has been demonstrated by Einspanier et al (2004). In the GIT of monogastrics, plant DNA is detectable in pigs (Klotz et al, 2002;Chowdury et al, 2003;Reuter and Aulrich, 2003), in chickens (Chambers et al, 2002) and in humans (Martìn-Orù e et al, 2002;Netherwood et al, 2004).…”

Section: Dnamentioning

confidence: 98%

Genetically modified soya bean in rabbit feeding: detection of DNA fragments and evaluation of metabolic effects by enzymatic analysis

et al. 2006

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The presence of DNA fragments in tissues from rabbits given genetically modified (GM) soya-bean meal (solvent extracted) was investigated by using the polymerase chain reaction (PCR) approach. Moreover, the possible effects on cell metabolism were evaluated by determination of several specific enzymes in serum, heart, skeletal muscle, liver and kidney. The chloroplast sequence for tRNA Leu by using the Clor1/Clor2 primers designed on chloroplast trnL sequence was clearly detected. On the contrary, two couples of species specific primers for conventional (Le1-5/Le 1-3 which amplifies the soya bean lectin gene) and genetically modified (35S1/35S2 which amplifies the 35S CMV promoter that is present in the genomic structure of GM soya bean) soya bean were not found in all samples. No differences in enzyme levels were detected in serum, but a significant increase of lactic dehydrogenase, mainly concerning the LDH1 isoenzyme was found in particular in kidney and heart but not in the muscle, thus suggesting a potential alteration in the local production of the enzyme. Finally, no significant differences were detected concerning body weight, fresh organ weights and no sexual differences were detected.

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Section: Dnamentioning

confidence: 98%

Genetically modified soya bean in rabbit feeding: detection of DNA fragments and evaluation of metabolic effects by enzymatic analysis

et al. 2006

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“…Deaville and Maddison (2005) reported that transgenic DNA from RR soybean meal or Bt maize was detectable in the broiler gizzard, but not in intestinal digesta. An experiment with broilers has shown that the bla gene (part of the Bt construct from Bt176 GM maize) was present in the gizzard content, but not in small intestine, caecum and rectum digesta (Chambers et al 2002). Such a high degradation and digestion of plant DNA in the alimentary tract of animals makes the possibility of GM DNA transfer to gut bacterial very improbable.…”

Section: Discussionmentioning

confidence: 99%

Assessing the possibility of genetically modified DNA transfer from GM feed to broiler, laying hen, pig and calf tissues

Sieradzki¹,

Mazur²,

Kwiatek³

et al. 2013

Polish Journal of Veterinary Sciences

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The aim of this study was to assess the possibility of genetically modified DNA transfer from feed containing RR soybean or/and MON810 maize to animal tissues, gut bacterial flora, food of animal origin, and the fate of GM DNA in the animal digestive tract. The experiment was carried out on broilers, laying hens, pigs and calves. All animals were divided into four groups: I -control group (non-modified feed), II -GM soybean group (non-modified maize, RR soybean), III -GM maize group (MON810 maize, non-modified soybean), and IV -GM maize and soybean group (MON810 maize, RR soybean). Samples of blood, organs, tissues, digesta from the gastrointestinal tract, and eggs were analysed for the presence of plant species specific genes, and transgenic sequences of CaMV 35S promoter and NOS terminator. PCR amplifications of these GM sequences were conducted to investigate the GM DNA transfer from feed to animal tissues and bacterial gut flora. In none of the analysed samples of blood, organs, tissues, eggs, excreta and bacterial DNA were plant reference genes or GM DNA found. A GM crop diet did not affect bacterial gut flora as regards diversity of bacteria species, quantity of particular bacteria species in the animal gut, or incorporation of transgenic DNA to the bacteria genome. It can be concluded that MON810 maize and RR soybean used for animal feeding are substantially equivalent to their conventional counterparts. Genetically modified DNA from MON810 maize and RR soybean is digested in the same way as plant DNA, with no probability of its transfer to animal tissues or gut bacterial flora.

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“…In an in vitro forced filter mating study, Netherwood et al (1999) found that gene transfer occurred at a rate of less than 10 -8 . In order for horizontal transfer to occur, DNA must first be released from the donor organism in a form which can be incorporated into the genome of a putative recipient bacterium and must remain intact for a sufficient time for the transfer to occur (Chambers et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

<i>In vivo</i> study on the persistence of transformed β-glucanase-producing <i>Lactobacillus</i> strains in the gastrointestinal tract of chickens

Sieo¹,

Abdullah²,

Tan³

et al. 2006

J. Anim. Feed Sci.

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The persistence and ability of transformed Lactobacillus strains to function and produce β-glucanase in the gastrointestinal tract of chickens was investigated. Chickens were fed diets supplemented with 10 9 cells/kg of transformed Lactobacillus strains which harboured plasmid pSA3 carrying a gene encoding β-glucanase. The transformed β-glucanase-producing Lactobacillus strains were L. brevis C10CpSA3b6, L.crispatus I12pSA3b6, L. brevis I23pSA3b6, L. fermentum I25pSA3b6, L. brevis I211pSA3b6 and L. brevis I218pSA3b6. At days 14 to 18, digesta samples from the gizzard, duodenum, jejunum, ileum and caecum were collected daily and the presence of the respective β-glucanase-producing Lactobacillus strains was determined by a PCR-based method. Results of the study showed that the β-glucanase-producing Lactobacillus strains could persist in the gastrointestinal tract of the chickens for about two days. The strains were found to be alive and active in vivo, in which β-glucanase activity was significantly (P<0.05) higher in chickens fed diets supplemented with β-glucanase-producing Lactobacillus strains. Higher enzyme activities were located in the jejunum and ileum, especially in birds fed β-glucanase-producing L. brevis C10CpSA3b6 and L. brevis I211pSA3b6. The results indicated that the Lactobacillus strains could be a potential vehicle to deliver enzymes into the gastrointestinal tract of chickens.

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The fate of antibiotic resistance marker genes in transgenic plant feed material fed to chickens

Cited by 47 publications

References 7 publications

Genetically modified soya bean in rabbit feeding: detection of DNA fragments and evaluation of metabolic effects by enzymatic analysis

Genetically modified soya bean in rabbit feeding: detection of DNA fragments and evaluation of metabolic effects by enzymatic analysis

Assessing the possibility of genetically modified DNA transfer from GM feed to broiler, laying hen, pig and calf tissues

<i>In vivo</i> study on the persistence of transformed β-glucanase-producing <i>Lactobacillus</i> strains in the gastrointestinal tract of chickens

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