The fate of certain organic acids and amides in the rabbit. 10. The application of paper chromatography to metabolic studies of hydroxybenzoic acids and amides

Bray, H. G.; Thorpe, W. V.; White, Kenneth L.

doi:10.1042/bj0460271

Cited by 236 publications

(12 citation statements)

References 11 publications

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“…They were sprayed with diazotized sulphanilic acid (Bray, Thorpe & White, 1950 Dagley & Pate1 (1957). The precipitates obtained were separated by centrifugation at 20,OOOg for 30 min.…”

Section: Methodsmentioning

confidence: 99%

The Metabolism of Aromatic Compounds Related to Lignin by some Hyphomycetes and Yeast-like Fungi of Soil

Henderson¹

1961

Journal of General Microbiology

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SUMMARYThe metabolism of various lignin-related aromatic compounds by several soil Hyphomycetes and yeast-like fungi was investigated. Adaptation studies with whole organisms and cell-free extracts confirmed previously proposed metabolic pathways (Henderson & Farmer, 1955;Henderson, 1960). It was shown that protocatechuic acid is an intermediate in the metabolism of vanillin and ferulic acid. Protocatechuic acid oxidase activity of cell-free extracts of Pullularia pullulans was found to be stimulated by ferrous ions and to depend on -SH groups.

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“…They were sprayed with diazotized sulphanilic acid (Bray, Thorpe & White, 1950 Dagley & Pate1 (1957). The precipitates obtained were separated by centrifugation at 20,OOOg for 30 min.…”

Section: Methodsmentioning

confidence: 99%

The Metabolism of Aromatic Compounds Related to Lignin by some Hyphomycetes and Yeast-like Fungi of Soil

Henderson¹

1961

Journal of General Microbiology

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“…a t 21O, by the descending method. They were sprayed with diazotized sulphanilic acid (Bray, Thorpe & White, 1950), ammoniacal silver nitrate or buffer spray (Fewster & Hall, 1951) for the detection of residual substrates, and of intermediates when these were known from previous investigations (Henderson & Farmer, 1955;Henderson, 1957). When deficiency of a particular metal was affecting metabolism, the differences in rates of metabolism were so distinct that they could readily be detected by examination of the size and intensity of spots obtained on paper chromatograms.…”

Section: E K Hendersonmentioning

confidence: 99%

The Influence of Trace Elements on the Metabolism of Aromatic Compounds by Soil Fungi

Henderson¹

1960

Journal of General Microbiology

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SUMMARYThe effect of copper, iron, manganese and zinc on the metabolism of catechol, o-, m-and p-hydroxybenzoic, o-, m-and p-methoxybenzoic, 3 :4-dimethoxybenzoic and vanillic acids was investigated. Aspergillus niger, Hormodendrum sp. and Penicillium sp. mycelia which were deficient in the various trace elements were the organisms used. Only iron had any marked effect on the rates of metabolism and on the accumulation of intermediate products of metabolism in the substrate solutions. The intermediate products identified were : protocatechuic acid from mand p-hydroxybenzoic and p-methoxybenzoic acids ; catechol from o-hydroxybenzoic acid; vanillic acid from 3:$-dimethoxybenzoic acid; o-, m-and p-hydroxybenzoic acids from the corresponding mono-methoxybenzoic acids.

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“…After being flushed with 02, the assay mixes were incubated with shaking at 30 °C for 1-3 h. The reaction products were extracted into chloroform and separated by thin-layer chromatography (TLC) using benzene-methanol (19 : 1, v/v) as solvents [9]. Phenolic compounds were detected on the chromatogram by staining with diazotized p-nitroaniline followed by NaHCO3 [2] or by autoradiography with Fuji X-ray film for 1-14 days.…”

Section: 4-d Monooxygenase Assaymentioning

confidence: 99%

Expression of a bacterial gene in transgenic tobacco plants confers resistance to the herbicide 2,4-dichlorophenoxyacetic acid

et al. 1989

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Plants resistant to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) were produced through the genetic engineering of a novel detoxification pathway into the cells of a species normally sensitive to 2,4-D. We cloned the gene for 2,4-D monooxygenase, the first enzyme in the plasmid-encoded 2,4-D degradative pathway of the bacterium Alcaligenes eutrophus, into a cauliflower mosaic virus 35S promoter expression vector and introduced it into tobacco plants by Agrobacterium-mediated transformation. Transgenic tobacco plants expressing the highest levels of the monooxygenase enzyme exhibited increased tolerance to 2,4-D in leaf disc and seed germination assays, and young plants survived spraying with levels of herbicide up to eight times the usual field application rate. The introduction of the gene for 2,4-D monooxygenase into broad-leaved crop plants, such as cotton, should eventually allow 2,4-D to be used as an inexpensive post-emergence herbicide on economically important dicot crops.

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The fate of certain organic acids and amides in the rabbit. 10. The application of paper chromatography to metabolic studies of hydroxybenzoic acids and amides

Cited by 236 publications

References 11 publications

The Metabolism of Aromatic Compounds Related to Lignin by some Hyphomycetes and Yeast-like Fungi of Soil

The Metabolism of Aromatic Compounds Related to Lignin by some Hyphomycetes and Yeast-like Fungi of Soil

The Influence of Trace Elements on the Metabolism of Aromatic Compounds by Soil Fungi

Expression of a bacterial gene in transgenic tobacco plants confers resistance to the herbicide 2,4-dichlorophenoxyacetic acid

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