The fate of DNA of transgenic inulin synthesizing potatoes in pigs

Abstract

Silage from a genetically modified potato expressing the 1-SST (sucurose:sucrose 1-fructosyltransferase) and the 1-FFT (fructan:fructan 1-fructosyltransferase) was used in a feeding experiment with pigs. After a feeding period of 42 days samples from various organs and digesta were collected and investigated with four different real time PCR systems, in order to identify the fate of the foreign DNA. No plant specific DNA or DNA specific for the genome alteration in the transgenic potato were detected in any organ. In contrast, chloroplast specific DNA was detected in the digesta of duodenum, jejunum, colon and rectum. The single-copy metallo-carboxypeptidase inhibitor gene sequence was detected only in samples from the stomach content of pigs fed the isogenic potato and in those from duodenum and jejunum of animals fed the transgenic one. No evidence for the integration of the foreign DNA into the host genome was observed.

Detection of genetically modified food in digesta and organs of rats fed transgenic potato

“…El-Sanhoty et al (2006) showed that segments from transgenic potato lines could not be found in tissue samples although the diets were offered in the mash form without degradation of the transgenic potato and chloroplast plant DNA. Broll et al (2005) detected no plant specific DNA or recombinant DNA in the organ of pigs fed transgenic potato. These authors also found chloroplast-specific DNA in the digesta of the duodenum, jejunum, colon and rectum, and reported no evidence for integration transgenes in the host.…”

“…These authors also found chloroplast-specific DNA in the digesta of the duodenum, jejunum, colon and rectum, and reported no evidence for integration transgenes in the host. The presence of recombinant DNA from transgenic plants in the organs and tissues of animals fed transgenic feed has been previously reported as rare (Chowdhury et al, 2003;Broll et al, 2005;Mazza et al, 2005;Sharma et al, 2006;Swiatkiewicz et al, 2014). Sharma et al (2006) reported that native plant DNA and the cp4-epsps transgene can be detected in the GIT digesta and in tissues and organs of fish and pigs fed transgenic Roundup-Ready canola meal.…”

Distortion of Genetically Modified Organism Quantification in Processed Foods: Influence of Particle Size Compositions and Heat-Induced DNA Degradation

“…CI (P<0.05) : confidence interval at 95% probability. of GMO contents in unprocessed reference materials but showed a significant underestimation of GMO contents in heat-sterilized samples (31,32). The fact that the transgenic specific target sequence (129 bp) was longer than that used for the detection of the reference gene (79 bp) permitted the assumption that distortions in the results of relative quantification could result from an unequal sensitivity of target sequences toward processing parameters.…”

“…This left the uncertainty whether the found discrepancies in quantification results were induced as a consequence of DNA degradation or if these are to be seen as inherent bias of the quantification system. Results of an international ring trial regarding the validation of a method for the quantification of transgenic maize (line Bt176) allowed an accurate quantification of GMO contents in unprocessed reference materials but showed a significant underestimation of GMO contents in heat-sterilized samples (31,32). The fact that the transgenic specific target sequence (129 bp) was longer than that used for the detection of the reference gene (79 bp) permitted the assumption that distortions in the results of relative quantification could result from an unequal sensitivity of target sequences toward processing parameters.…”

Nutritional properties of tubers of conventionally bred and transgenic lines of potato resistant to necrotic strain of Potato virus Y (PVYN).

“…In an experiment of Böhme and coworkers (2005), genetic transformation of inulin synthesis in potatoes resulted in depression of starch content and fibre digestibility for pigs. A�er a feeding period of 42 days no plant DNA or DNA specific for the genome alteration in the transgenic potato were detected in gastrointestinal digesta or in the internal organs of pigs (Broll et al, 2005).…”