The fate of human malignant melanoma cells transplanted into zebrafish embryos: Assessment of migration and cell division in the absence of tumor formation

Lee, Lisa M.J.; Seftor, Elisabeth A.; Bonde, Gregory; Cornell, Robert A.; Hendrix, Mary J.C.

doi:10.1002/dvdy.20471

Cited by 276 publications

(243 citation statements)

References 21 publications

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“…Transplantation of human cancer cells into early-stage (6-48 h post-fertilization) zebrafish embryos has also revealed important information about cancer biology as reported by several groups (Haldi et al, 2005;Lee et al, 2005;Topczewska et al, 2006;Nicoli et al, 2007). The major advantage of this model is that the immune system is still immature that permits human tumor cell engraftment.…”

Section: Early Embryo Xenotransplantant Modelmentioning

confidence: 95%

“…Cancer cells derived from various species (mouse and human) and tissues (adenocarcinoma, fibrosarcoma, melanoma) have been introduced into both young embryos (2-5-days old post fertilization; Haldi et al, 2005;Lee et al, 2005;Topczewska et al, 2006;Nicoli et al, 2007) and juvenile 30-day-old zebrafish (Stoletov et al, 2007). Both transplantation models have their specific benefits and limitations when it comes to understanding cancer cell behavior in a living organism (Table 1).…”

Section: Xenotransplantant Modelsmentioning

confidence: 99%

“…Early embryo model: tumor cell plasticity Using young embryos, Lee et al (2005) found that transplanted human melanoma cells were highly invasive and randomly scatter within the embryonic tissue, whereas primary melanocytes were often integrated normally with the developing skin. When examined for expression of melanocyte differentiation factors in vivo, aggressive melanoma cells were found to remain in a dedifferentiated state expressing both mesenchymal and epithelial markers.…”

Section: Early Embryo Xenotransplantant Modelmentioning

confidence: 99%

“…Cancer progression in these animals recapitulates many aspects of human disease and opens the door for studies to identify genetic and chemical modifiers of cancer (Amatruda et al, 2002;Smolowitz et al, 2002;Stern and Zon, 2003;Berghmans et al, 2005a;Goessling et al, 2007a;Kari et al, 2007). The attention has been further fueled by the development of xenograft models that allow the propagation and visualization of human cancer cells engrafted in optically transparent zebrafish (Haldi et al, 2005;Lee et al, 2005;Topczewska et al, 2006;Nicoli et al, 2007;Stoletov et al, 2007). The integration of zebrafish genetics with the large tool chest of reagents available to study human cancer cells provides a powerful new vertebrate model to visualize and dissect the mechanisms that drive cancer formation, angiogenesis and metastasis.…”

Section: Introductionmentioning

confidence: 99%

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Catch of the day: zebrafish as a human cancer model

2008

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Zebrafish are making big waves in the field of cancer research. The effect has been widespread and continues to gain speed as more and more cancer researchers ride the wave of zebrafish biology. This has been largely due to the development of transgenic and xenograft models of cancer, which recapitulate many aspects of different human cancers including lymphoblastic T-cell leukemia, pancreatic cancer, melanoma and rhabdomyosarcoma. These models are already being utilized by academia and industry to search for genetic and chemical modifiers of cancer with success. The attention has been further stimulated by the amenability of zebrafish to pharmacological testing and the superior imaging properties of fish tissues that allow visualization of cancer progression and angiogenesis in live animals. This review summarizes the current zebrafish models of cancer and discusses their utility in human cancer research and future directions in the field.

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Section: Early Embryo Xenotransplantant Modelmentioning

confidence: 95%

Section: Xenotransplantant Modelsmentioning

confidence: 99%

Section: Early Embryo Xenotransplantant Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Catch of the day: zebrafish as a human cancer model

2008

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“…1 --5 Lee et al 6 demonstrated that exposure of metastatic melanoma cells to an embryonic zebrafish microenvironment, before gastrulation, results in their reprogramming toward a nontumorigenic phenotype. In addition, when metastatic melanoma cells were transplanted into the chick embryonic microenvironment, the melanoma cells invaded host neural crest targets but did not form tumors, and a subset of these tumor cells were reprogrammed to a neural crest cell-like phenotype.…”

Section: Introductionmentioning

confidence: 99%

Role of Lefty in the anti tumor activity of human adult liver stem cells

et al. 2012

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Recent studies demonstrated that factors derived from embryonic stem cells inhibit the tumorigenicity of a variety of cancer cell lines. Embryonic stem cell-secreted Lefty, an inhibitor of Nodal-signalling pathway, was implicated in reprogramming cancer cells. Whether adult stem cells exhibited similar properties has not been explored. The aim of the present study was to investigate whether the conditioned medium (CM) derived from adult stem cells influence in vitro and in vivo tumor growth by a Nodal-dependent pathway. In particular we compared the anti-tumor effect of CM from human liver stem cells (HLSC) with that of bone marrow-derived mesenchymal stem cells (MSC). We found that HLSC-CM inhibited the in vitro growth and promoted apoptosis in HepG2 cells that expressed a deregulated Nodal pathway. The effect of HLSC-CM was related to the presence of Lefty A in the CM of HLSC. Silencing Lefty A in HLSC or Lefty A blockade with a blocking peptide abrogated the anti-proliferative and pro-apoptotic effect of HLSC-CM. Moreover, the administration of human recombinant Lefty A protein mimicked the effect of HLSC-CM indicating that Nodal pathway is critical for the growth of HepG2. At variance of HLSC, bone marrow-derived MSC did not express and release Lefty A and the MSC-CM did not exhibited an anti-tumor activity in vitro, but rather stimulated proliferation of HepG2. In addition, the intra-tumor administration of HLSC-CM was able to inhibit the in vivo growth of HepG2 hepatoma cells implanted subcutaneously in SCID mice. At variance, HLSC-CM derived from Lefty A silenced HLSC was unable to inhibit tumor growth. In conclusion, the results of present study suggest that Lefty A may account for the tumor suppressive activity of HLSC as a result of an inhibition of the Nodal-signalling pathway by a mechanism similar to that described for embryonic stem cells.

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Models for Skin Cancer

Philpott

Bergamaschi

Storey

2007

The Cancer Handbook

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Malignant melanoma, basal cell carcinoma (BCC), and squamous cell carcinoma (SCC) are the three most common types of skin cancer. Malignant melanoma commonly arises from melanocytes in the epidermis and is the most lethal of skin cancers. BCC arises from keratinocytes and is the commonest cancer. There is considerable debate as to whether BCC arises from hair follicle keratinocytes and also because of the association of BCC with mutations that activate the sonic hedgehog (SHH) pathway, whether BCC may reflect failed organogenesis. SCC also arises from keratinocytes and is a more aggressive tumour than BCC and can form lethal metastases, whereas BCC rarely metastasize. All three skin cancers lend themselves to both in vivo and in vitro models and these are discussed in this chapter.

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The fate of human malignant melanoma cells transplanted into zebrafish embryos: Assessment of migration and cell division in the absence of tumor formation

Cited by 276 publications

References 21 publications

Catch of the day: zebrafish as a human cancer model

Catch of the day: zebrafish as a human cancer model

Role of Lefty in the anti tumor activity of human adult liver stem cells

Models for Skin Cancer

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