The Fate of Integrated Ri T-DNA<i> rol</i> Genes during Regeneration via Somatic Embryogenesis in<i> Tylophora indica</i>

Roychowdhury, Dipasree; Chaubey, Binay; Jha, Sumita

doi:10.1155/2015/707831

Cited by 5 publications

(1 citation statement)

References 51 publications

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“…As previously reported, molecular analysis of these RLs con rmed the presence of T L and T R T-DNA parts, represented by rolB, rolC, aux and ags respectively 26 . This suggests that biosynthetic capacity for accumulation of different classes of SMs can be due to the rolC gene-induced from the A. rhizogenes plasmid 51 . But the real mechanism of advancement is still not well-founded and needs more systematic studies of expression of critical enzymes involved in superior metabolic pathways.…”

Section: Discussionmentioning

confidence: 98%

Interval harvests changes phytochemical traits and biological activities of elite transformed hairy root cultures of African geranium (Pelargonium sidoides DC.)

Yousefian

Hamidoghli

Golkar

et al. 2022

Preprint

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Tuberous roots of African geranium (Pelargonium sidoides DC.) with modern ethanolic formulation called EPs 7630 (Umckaloabo) have widely used in medicinal industries. The present study was aimed to explore changes in the phytochemical traits (i.e., phenolics, flavonoids, coumarins and hydroxycinnamic acid derivatives), protein content, enzymatic-antioxidants and antimicrobial activities of several high-yielding hairy root lines (HRLs) of P. sidoides harvested at different times. Among all HRLs, antioxidative responses (e.g., phenylalanine ammonia lyase, polyphenol oxidase and catalase contents) were increased in HRL10 exposed to higher interval harvests. Moreover, HRL10 exhibited a promising antimicrobial effect against Escherichia coli, with MIC equal to (2.61 mg/ml). In total 10 phytochemical compounds were identified through chromatographic (HPLC) technique including: umckalin (Um), gallic acid (GA), quercetin (QE), luteolin (LU) and the others. The extraction of HRs had high content of Um at 21 days (0.35 𝜇g/ml). The maximal phenolic yield in particular GA was enhanced to (5194.5 𝜇g/ml), about 10-fold in comparison with non-transformed roots (516 𝜇g/ml) on the 35th day. HRs were able to produce the maximum improvement in flavonoid glycosides (13.96 𝜇g QE/ml and 23.75 𝜇g LU/ml) at the end of culture period. The most profitable transgenic lines offers an interesting option to enhance the productivity. These results support the in vitro cultivation of HRs for supply as a natural product to prevent inflammation and microbial related disorders.

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Section: Discussionmentioning

confidence: 98%

Interval harvests changes phytochemical traits and biological activities of elite transformed hairy root cultures of African geranium (Pelargonium sidoides DC.)

Yousefian

Hamidoghli

Golkar

et al. 2022

Preprint

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The Effects of rol Genes of Agrobacterium rhizogenes on Morphogenesis and Secondary Metabolite Accumulation in Medicinal Plants

Sarkar

Ghosh

Roychowdhury³

et al. 2018

Biotechnological Approaches for Medicinal and Aromatic Plants

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Micropropagation and genetic transformation of Tylophora indica (Burm. f.) Merr.: a review

Silva¹,

Jha

2016

Plant Cell Rep

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This review provides an in-depth and comprehensive overview of the in vitro culture of Tylophora species, which have medicinal properties. Tylophora indica (Burm. f.) Merr. is a climbing perennial vine with medicinal properties. The tissue culture and genetic transformation of T. indica, which has been extensively studied, is reviewed. Micropropagation using nodal explants has been reported in 25 % of all publications. Leaf explants from field-grown plants has been the explant of choice of independent research groups, which reported direct and callus-mediated organogenesis as well as callus-mediated somatic embryogenesis. Protoplast-mediated regeneration and callus-mediated shoot organogenesis has also been reported from stem explants, and to a lesser degree from root explants of micropropagated plants in vitro. Recent studies that used HPLC confirmed the potential of micropropagated plants to synthesize the major T. indica alkaloid tylophorine prior to and after transfer to field conditions. The genetic integrity of callus-regenerated plants was confirmed by RAPD in a few reports. Tissue culture is an essential base for genetic transformation studies. Hairy roots and transgenic T. indica plants have been shown to accumulate tylophorine suggesting that in vitro biology and transgenic methods are viable ways of clonally producing valuable germplasm and mass producing compounds of commercial value. Further studies that investigate the factors affecting the biosynthesis of Tylophora alkaloids and other secondary metabolites need to be conducted using non-transformed as well as transformed cell and organ cultures.

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The Fate of Integrated Ri T-DNA rol Genes during Regeneration via Somatic Embryogenesis in Tylophora indica

Cited by 5 publications

References 51 publications

Interval harvests changes phytochemical traits and biological activities of elite transformed hairy root cultures of African geranium (Pelargonium sidoides DC.)

Interval harvests changes phytochemical traits and biological activities of elite transformed hairy root cultures of African geranium (Pelargonium sidoides DC.)

The Effects of rol Genes of Agrobacterium rhizogenes on Morphogenesis and Secondary Metabolite Accumulation in Medicinal Plants

Micropropagation and genetic transformation of Tylophora indica (Burm. f.) Merr.: a review

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