The fate of mechanically induced cartilage in an unloaded environment

Rooij, Philippe P. de; Siebrecht, Maikel A.N; Tägil, Magnus; Aspenberg, Per

doi:10.1016/s0021-9290(01)00044-6

Cited by 29 publications

(12 citation statements)

References 12 publications

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“…The finding in this study that the response to loading is donor dependent may in part explain some of this variability. Perhaps this result is to be expected given the well documented variability in the response of MSCs from different donors to cytokine induced chonodrogenic differentiation (Cicione et al 2010), and the fact that animal model studies investigating mechanically induced chondrogenesis in vivo often report dramatic donor dependant response to loading (Khayyeri et al ;Tägil and Aspenberg 1999;De Rooij et al 2001). Further studies are required to better understand donor variability in the response to mechanical signals that clearly cannot be determined based on an analysis of only a small number of donors.…”

Section: Discussionmentioning

confidence: 96%

The effect of cyclic hydrostatic pressure on the functional development of cartilaginous tissues engineered using bone marrow derived mesenchymal stem cells

Meyer

Buckley

Steward

et al. 2011

Journal of the Mechanical Behavior of Biomedical Materials

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The effect of cyclic hydrostatic pressure on the functional development of cartilaginous tissues engineered using bone marrow derived mesenchymal stem cells. Journal of the Mechanical Behavior of Biomedical Materials (2011Materials ( ), doi:10.1016Materials ( /j.jmbbm.2011 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.T he effect of cyclic hydrostatic pressure on the functional development of cartilaginous tissues engineered using bone mar row derived mesenchymal stem cells *Manuscript Click here to view linked References A bstractMechanical signals can play a key role in regulating the chondrogenic differentiation of Mesenchymal Stem Cells (MSCs). The objective of this study was to determine if the longterm application of cyclic hydrostatic pressure could be used to improve the functional properties of cartilaginous tissues engineered using bone marrow derived MSCs. MSCs were isolated from the femora of two porcine donors, expanded separately under identical conditions, and then suspended in cylindrical agarose hydrogels. Constructs from both donors were maintained in a chemically defined media supplemented with TGF- increased dynamic modulus compared to FS controls. In contrast, CHP had no effect on matrix accumulation for the other donor. The application of DHP had no effect on either matrix accumulation or construct mechanical properties for both donors. Variability in the response to hydrostatic pressure was also observed for three further donors. In conclusion, this study demonstrates that the application of long term hydrostatic pressure can be used to improve the functional properties of cartilaginous tissues engineered using bone marrow derived MSCs by enhancing collagen and GAG accumulation. The response to such loading however is donor dependant, which has implications for the clinical utilization of such a stimulus when engineering cartilaginous grafts using autologous MSCs. IntroductionArticular cartilage has a poor capacity for repair. Of the procedures available to the orthopaedic surgeon, osteochondral grafting is the only technique which reliably produces hyaline cartilage within a defect (Getgood et al. 2009). This suggests that if tissue engineering strategies could be used to develop cartilaginous grafts with mechanical properties approaching that of normal articular cartilage, then hyaline tissue could be regenerated. There has been increased interested in using Mesenchymal Stem Cells ( The objective of this study is to determine the influence of cyclic hydrostatic pressure initiated either before or after TGFmaturation of cartilaginous grafts engineered using bone marrow derived ...

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Section: Discussionmentioning

confidence: 96%

The effect of cyclic hydrostatic pressure on the functional development of cartilaginous tissues engineered using bone marrow derived mesenchymal stem cells

Meyer

Buckley

Steward

et al. 2011

Journal of the Mechanical Behavior of Biomedical Materials

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“…6,18,19 Among these, Tä gil and Aspenberg's experiments on rats 18 are ideal for corroboration of tissue differentiation simulations, since a defined loading was used and considerable experimental work has been reported. 18,20,21 This chamber consists of a hollow screw with two ingrowth openings at the bottom from which mesenchymal progenitor cells can penetrate to fill the chamber. In the experiments, mechanical loading was applied via a piston, which exerted a pressure on the in-growing tissues (Fig.…”

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confidence: 99%

Corroboration of mechanobiological simulations of tissue differentiation in an in vivo bone chamber using a lattice‐modeling approach

Khayyeri

Checa

Tägil

et al. 2009

Journal Orthopaedic Research

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It is well established that the mechanical environment modulates tissue differentiation, and a number of mechanoregulatory theories for describing the process have been proposed. In this study, simulations of an in vivo bone chamber experiment were performed that allowed direct comparison with experimental data. A mechanoregulation theory for mesenchymal stem cell differentiation based on a combination of fluid flow and shear strain (computed using finite element analysis) was implemented to predict tissue differentiation inside mechanically controlled bone chambers inserted into rat tibae. To simulate cell activity, a lattice approach with stochastic cell migration, proliferation, and selected differentiation was adopted; because of its stochastic nature, each run of the simulation gave a somewhat different result. Simulations predicted the load-dependency of the tissue differentiation inside the chamber and a qualitative agreement with histological data; however, the full variability found between specimens in the experiment could not be predicted by the mechanoregulation algorithm. This result raises the question whether tissue differentiation predictions can be linked to genetic variability in animal populations. ß

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“…During development, both movement and mechanical load play a critical role in differentiating embryonic MSC into chondrocytes leading to development of the articular surface [199,214]. In young humans, morphological properties of articular structures are further defined (or developed) by mechanical loading of the joints [199,200].…”

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confidence: 99%

“…In young humans, morphological properties of articular structures are further defined (or developed) by mechanical loading of the joints [199,200]. Even in adulthood, physiological joint loading is necessary and responsible for the maintenance of articular structures and leads to varying mechanical properties between different joints and within each joint [200,214]. Variations in loading have been shown to alter gene expression, chondrocyte density, and biosynthetic response thereby resulting in different organization schemes of the constituents within the ECM [199,200,214].…”

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confidence: 99%

“…Even in adulthood, physiological joint loading is necessary and responsible for the maintenance of articular structures and leads to varying mechanical properties between different joints and within each joint [200,214]. Variations in loading have been shown to alter gene expression, chondrocyte density, and biosynthetic response thereby resulting in different organization schemes of the constituents within the ECM [199,200,214]. For example, regions such as the patellofemoral articulation that are subjected to high shear loads possess a high concentration of collagen fibrils in the superficial layer of the cartilage that are aligned in the direction of shear loading [199].…”

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confidence: 99%

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Cartilage tissue engineering for degenerative joint disease☆

Nešić

Whiteside

Brittberg

et al. 2006

Advanced Drug Delivery Reviews

211

156

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Pain in the joint is often due to cartilage degeneration and represents a serious medical problem affecting people of all ages. Although many, mostly surgical techniques, are currently employed to treat cartilage lesions, none has given satisfactory results in the long term. Recent advances in biology and material science have brought tissue engineering to the forefront of new cartilage repair techniques. The combination of autologous cells, specifically designed scaffolds, bioreactors, mechanical stimulations and growth factors together with the knowledge that underlies the principles of cell biology offers promising avenues for cartilage tissue regeneration. The present review explores basic biology mechanisms for cartilage reconstruction and summarizes the advances in the tissue engineering approaches. Furthermore, the limits of the new methods and their potential application in the osteoarthritic conditions are discussed.

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The fate of mechanically induced cartilage in an unloaded environment

Cited by 29 publications

References 12 publications

The effect of cyclic hydrostatic pressure on the functional development of cartilaginous tissues engineered using bone marrow derived mesenchymal stem cells

The effect of cyclic hydrostatic pressure on the functional development of cartilaginous tissues engineered using bone marrow derived mesenchymal stem cells

Corroboration of mechanobiological simulations of tissue differentiation in an in vivo bone chamber using a lattice‐modeling approach

Cartilage tissue engineering for degenerative joint disease☆

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