The Fate of Newly Synthesized Hormone from Neuroendocrine Cells ofAplysia

Lee, Wenjau; Wayne, Nancy L.

doi:10.1006/gcen.1997.6916

Cited by 6 publications

(17 citation statements)

References 48 publications

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“…5). Isolated bag cell clusters have been shown to exhibit normal electrical and secretory properties for days in culture medium (14). This result further demonstrates the durable nature of these neuroendocrine cells.…”

Section: Stability Of Elh Mrnasupporting

confidence: 57%

“…Previous work showed that newly synthesized ELH is preferentially released within 24 h of being made, and that this new hormone contributes to half of the total amount of ELH released in response to afterdischarge (14). This suggests that the synthetic capacity of the bag cell neurons is quite high, especially during the breeding season when animals are laying eggs and secreting large amounts of ELH on a near-daily basis (15,16).…”

mentioning

confidence: 86%

“…However, when the stimulation of glucose continues for a longer period (Ͼ6 h), the preproinsulin mRNA level (33) and its stability (34) increase. In our previous study (14), 4 consecutive days of afterdischarge caused a decrease in the bag cell content of ELH. Thus, increased ELH biosynthesis appears to be unable to fully compensate for secretion-induced loss of ELH.…”

Section: Fig 4 Effects Of Afterdischarge (Ad)mentioning

confidence: 99%

“…One of the bilateral clusters was treated with 50 g/ml actinomycin D for 2,4,8,16, and 32 h (n ϭ 4 -5/group), and the other served as an untreated control. We have demonstrated in a previous study that bag cell clusters maintained in culture for up to 5 days showed normal electrical and secretory properties (14). Therefore, bag cell functions should not decline over the course of 32 h. After incubation, the clusters were homogenized; total RNA was isolated and processed for Northern blot analysis of ELH mRNA.…”

Section: Effects Of Transcription Inhibitor and Translation Inhibitormentioning

confidence: 99%

“…A 10-to 30-min afterdischarge stimulates ELH secretion over a 1-to 2-h period, during which time hundreds to thousands of nanograms of ELH are released (13,14). Previous work showed that newly synthesized ELH is preferentially released within 24 h of being made, and that this new hormone contributes to half of the total amount of ELH released in response to afterdischarge (14).…”

mentioning

confidence: 99%

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The Roles of Transcription and Translation in Mediating the Effect of Electrical Afterdischarge on Neurohormone Synthesis inAplysiaBag Cell Neurons¹

Lee

Wayne

1998

Endocrinology

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The molecular links between membrane excitability and neurohormone synthesis were investigated using a simple model system: the bag cell neurons of Aplysia. We tested the hypothesis that the electrical afterdischarge, which leads to depletion of egg-laying hormone (ELH) by triggering secretion, rapidly stimulates ELH synthesis to replenish bag cell stores of hormone. Newly synthesized peptides were radiolabeled, and ELH peptide was immunoprecipitated. Within 4 h of afterdischarge, there was a 100% increase in ELH synthesis compared with that in unstimulated controls. Northern blot analysis showed that ELH messenger RNA (mRNA) levels were not altered for at least 8 h after the onset of afterdischarge. ELH mRNA levels were also not affected by 4-h treatments that inhibited either transcription (with actinomycin D) or translation (with anisomycin). Further work revealed that ELH mRNA is stable, with a half-life exceeding 32 h. Notably, the stimulatory effect of afterdischarge on ELH synthesis was blocked in response to treatment with the transcription inhibitor actinomycin D. These results suggest that the afterdischarge-induced increase in ELH synthesis is mediated by an increase in the rate of translation of already existing ELH mRNA, and that transcription of a non-ELH gene(s) is required for this effect to occur. (Endocrinology 139: 5109 -5115, 1998)

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Section: Stability Of Elh Mrnasupporting

confidence: 57%

mentioning

confidence: 86%

Section: Fig 4 Effects Of Afterdischarge (Ad)mentioning

confidence: 99%

Section: Effects Of Transcription Inhibitor and Translation Inhibitormentioning

confidence: 99%

mentioning

confidence: 99%

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The Roles of Transcription and Translation in Mediating the Effect of Electrical Afterdischarge on Neurohormone Synthesis inAplysiaBag Cell Neurons¹

Lee

Wayne

1998

Endocrinology

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Gonadotropin-releasing hormone in invertebrates: Structure, function, and evolution

Tsai

2006

General and Comparative Endocrinology

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Regional differences in processing of locally translated prohormone in peptidergic neurons of Aplysia californica

Lee

Jones

Ono

et al. 2002

Journal of Neurochemistry

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Earlier work showed that cell bodies and neurites of the peptidergic bag cell neurons of Aplysia californica contain mRNA for egg-laying hormone. The purpose of the present study was to determine if egg-laying hormone synthesis and prohormone processing is similar in the pleurovisceral connective nerves (containing neurites of bag cell neurons) and the bag cell neuron clusters (containing both cell bodies and neurites of bag cell neurons). Initial experiments confirmed by RT-PCR and sequencing that egg-laying hormone mRNA was present in the pleurovisceral connective nerves. To investigate possible regional differences in translation of mRNA and prohormone processing, clusters were separated from connective nerves and newly synthesized egg-laying hormoneimmunoreactive proteins were analyzed. Results showed that synthesis and processing of prohormone occurred in both the clusters and isolated connective nerves; however, the relative abundance of prohormone, processing intermediates, and egg-laying hormone was different. Pulse-chase experiments showed that prohormone was processed more slowly in the connective nerves than in the clusters. These results show that mRNA in isolated neural processes of neuroendocrine cells can be translated, and that the cellular machinery for protein synthesis is present, but processing of the ELH prohormone is significantly compromised.

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The Fate of Newly Synthesized Hormone from Neuroendocrine Cells ofAplysia

Cited by 6 publications

References 48 publications

The Roles of Transcription and Translation in Mediating the Effect of Electrical Afterdischarge on Neurohormone Synthesis inAplysiaBag Cell Neurons¹

The Roles of Transcription and Translation in Mediating the Effect of Electrical Afterdischarge on Neurohormone Synthesis inAplysiaBag Cell Neurons¹

Gonadotropin-releasing hormone in invertebrates: Structure, function, and evolution

Regional differences in processing of locally translated prohormone in peptidergic neurons of Aplysia californica

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The Fate of Newly Synthesized Hormone from Neuroendocrine Cells ofAplysia

Cited by 6 publications

References 48 publications

The Roles of Transcription and Translation in Mediating the Effect of Electrical Afterdischarge on Neurohormone Synthesis inAplysiaBag Cell Neurons1

The Roles of Transcription and Translation in Mediating the Effect of Electrical Afterdischarge on Neurohormone Synthesis inAplysiaBag Cell Neurons1

Gonadotropin-releasing hormone in invertebrates: Structure, function, and evolution

Regional differences in processing of locally translated prohormone in peptidergic neurons of Aplysia californica

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The Roles of Transcription and Translation in Mediating the Effect of Electrical Afterdischarge on Neurohormone Synthesis inAplysiaBag Cell Neurons¹

The Roles of Transcription and Translation in Mediating the Effect of Electrical Afterdischarge on Neurohormone Synthesis inAplysiaBag Cell Neurons¹