Changes in available phosphorus (as extracted by the Truog and Olsen methods) and in phosphorus held in the soil in aluminium-, iron-, and calcium-bound forms have been followed on soil samples taken from a rates of superphosphate trial on irrigated pasture from 1952 to 1964.Truog phosphorus levels in samples taken at three-monthly intervals showed a cyclic pattern due to the annual fertiliser applications, the fluctuations being greater at higher rates of superphosphate.In samples taken just before top-dressing each autumn, 3 cwt and 4t cwt of superphosphate applied annually caused a net increase in levels of aluminium-, iron-, and calcium-bound phosphorus, the aluminium-bound form increasing more than the other two forms. However. this form was also depleted at a greater rate than iron-or calcium-bound phosphorus during the residual phase of the trial. On these samples, the Truog and Olsen methods gave a pattern of phosphorus accumulation and depletion closely correlated with the aluminium-bound form, which is considered to be the most important source of phosphorus for plant uptake on this soil type.