The Fate of the Adipose-Derived Stromal Cells during Angiogenesis and Adipogenesis after Cell-Assisted Lipotransfer

Hong, Ki Yong; Yim, Sangjun; Kim, Hyun Jung; Jin, Ung Sik; Lim, SooA; Eo, SuRak; Chang, Hak; Minn, Kyung Won

doi:10.1097/prs.0000000000004021

Cited by 71 publications

(39 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further refinement in the “graft replacement” mechanism, was provided by Hong et al [ 97 ], who generated an animal model using two transgenic reporter mice expressing different fluorescent signals (DsRed and green fluorescent protein). Tracing experiments elucidated the dynamic changes of donor ASC, donor fat and recipient tissue.…”

Section: Clinical Approachmentioning

confidence: 99%

Autologous fat grafting: Latest insights

Doornaert

Colle

Maere

et al. 2019

Annals of Medicine and Surgery

View full text Add to dashboard Cite

A recent rise in the use of autologous fat transfer for soft tissue augmentation has paralleled the increasing popularity of liposuction body contouring. This creates a readily available and inexpensive product for lipografting, which is the application of lipoaspirated material. Consistent scientific proof about the long-term viability of the transferred fat is not available. Clinically, there is a reabsorption rate which has been reported to range from 20 to 90%. Results can be unpredictable with overcorrection and regular need for additional interventions. In this review, adipogenesis physiology and the adipogenic cascade from adipose-derived stem cells to adult adipocytes is extensively described to determine various procedures involved in the fat grafting technique. Variables in structure and physiology, adipose tissue harvesting- and processing techniques, and the preservation of fat grafts are taken into account to collect reproducible scientific data to establish standard in vitro and in vivo models for experimental fat grafting. Adequate histological staining for fat tissue, immunohistochemistry and viability assays should be universally used in experiments to be able to produce comparative results. By analysis of the applied methods and comparison to similar experiments, a conclusion concerning the ideal technique to improve clinical outcome is proposed.

show abstract

Section: Clinical Approachmentioning

confidence: 99%

Autologous fat grafting: Latest insights

Doornaert

Colle

Maere

et al. 2019

Annals of Medicine and Surgery

View full text Add to dashboard Cite

show abstract

“…The second hypothesis states that ASCs can induce angiogenesis and adipogenesis by differentiating into endothelial cells and adipocytes [48]. To elucidate the fate and microenvironmental changes of fat, ASCs, and recipient tissue associated with CAL, the author generated an experimental animal model using two different transgenic reporter mice, and grafted both fat and ASCs expressing different fluorescent signals into wild-type mice [1]. Tracing analyses revealed that the donor ASCs participated in angiogenesis by differentiating into endothelial cells.…”

Section: Mechanismmentioning

confidence: 99%

Fat grafts enriched with adipose-derived stem cells

Hong

2020

Arch Craniofac Surg

Self Cite

View full text Add to dashboard Cite

Autologous fat grafts have been widely used in soft-tissue augmentation and reconstruction surgery. Despite their numerous advantages, including a simple harvest technique, low cost, and easy accessibility, the applicability of autologous fat grafts is limited by their unpredictable long-term outcomes associated with poor graft retention [1,2]. In recent decades, several advances have been made to overcome the low rate of graft survival, including various refinements of existing techniques, the development of nanofat to reduce the size of fat particles used for injection, and the standardization of protocols for fat harvesting and processing [3-5]. Adipose tissue is composed of at least two functionally distinct types of fat: white and brown [6]. The primary roles of white adipose tissue are energy storage and the release of hormones and adipokines that modulate whole-body metabolism [7]. Consequently, the majority of studies related to adipose tissue have focused on the treatment of obesity, which contributes to an increased risk of developing type 2 diabetes, cardiovascular disorders, and cancer [8]. Additionally, white tissue can act as a thermal insulator and protect other organs from mechanical damage [9]. However, for plastic surgeons, the physical properties of white adipose tissue, not its function, make autologous fat grafts a useful component of their surgical strategies. Fate of fat grafts Adipose tissue is composed of adipocytes, adipose-derived stem/stromal cells (ASCs) and various other cells, including endothelial, mural, immune, and neuronal cells [10]. In addition, adipose tissue is highly vascularized, as each adipocyte is surrounded by an extensive capillary network [11]. Thus, angiogenesis is closely related to the maintenance and remodeling of adipose tissue. A recent study investigating the fate of adipocytes and ASCs after non-vascularized fat grafts identified three zones (survival, regenerating, and necrotic) in grafts [12]. In the survival zone, which is less than 300 μm thick, both adipocytes Archives of Craniofacial Surgery

show abstract

“…Another factor that may contribute to the proangiogenic effect seen with lipotransfer into irradiated tissues is the inclusion of additional vessel-forming elements [ 56 ]. These include endothelial cells, pericytes, smooth muscle cells, and their progenitors capable of forming vascular cells and blood vessels.…”

Section: Autologous Lipotransfer To Regenerate Radiated Tissuesmentioning

confidence: 99%

Lipotransfer Strategies and Techniques to Achieve Successful Breast Reconstruction in the Radiated Breast

Crawford

Endara

2020

Medicina

View full text Add to dashboard Cite

Radiation therapy is frequently a critical component of breast cancer care but carries with it side effects that are particularly damaging to reconstructive efforts. Autologous lipotransfer has the ability to improve radiated skin throughout the body due to the pluripotent stem cells and multiple growth factors transferred therein. The oncologic safety of lipotransfer to the breasts is demonstrated in the literature and is frequently considered an adjunctive procedure for improving the aesthetic outcomes of breast reconstruction. Using lipotransfer as an integral rather than adjunctive step in the reconstructive process for breast cancer patients requiring radiation results in improved complication rates equivalent to those of nonradiated breasts, expanding options in these otherwise complicated cases. Herein, we provide a detailed review of the cellular toxicity conferred by radiotherapy and describe at length our approach to autologous lipotransfer in radiated breasts.

show abstract

The Fate of the Adipose-Derived Stromal Cells during Angiogenesis and Adipogenesis after Cell-Assisted Lipotransfer

Cited by 71 publications

References 33 publications

Autologous fat grafting: Latest insights

Autologous fat grafting: Latest insights

Fat grafts enriched with adipose-derived stem cells

Lipotransfer Strategies and Techniques to Achieve Successful Breast Reconstruction in the Radiated Breast

Contact Info

Product

Resources

About