The favourable effect of catechin in electrochemotherapy in human pancreatic cancer cells

Abstract

Until recently, green tea polyphenols were considered strong antioxidants. However, the latest reports have revealed that bioflavonoids can play a multiple role in anticancer therapy, including the inhibition of cell proliferation and generation of the oxidative stress in a dose-dependent manner. The presented research was designed to examine the potential of the green tea (±)-catechin as a reinforcement of the electrochemotherapy (ECT) with cisplatin in pancreatic cancer in vitro. The study was performed on two cell lines of the pancreatic ductal adenocarcinoma (PDA) - parental EPP85-181P and multidrug-resistant EPP85-181RNOV. Prior to the ECT protocol the cells were preincubated with high or low concentration of catechin for 2 or 24 hours, respectively. We assessed the influence of preincubation on the cisplatin toxicity with and without electroporation (EP), the electrosensitivity of PDA cell lines and the uptake of the daunorubicin and propidium iodide. Additionally, we evaluated the antioxidative properties of catechin by the measurement of the ROS-related fluorescence and the immunoreactivity of the oxidative stress-related enzymes superoxide dismutase (SOD2) and glutathione S-transferase (GST). We found that co-treatment with catechin can firmly enhance the efficacy of electroporation with cisplatin in vitro. More favorable effect was obtained for 2-hour incubation, which indicates the involvement of the transcriptional-independent mechanisms of catechin action. The effect may be partially explained by the increased oxidative stress level, which was higher in multidrug-resistant cells. However, further studies on cisplatin-catechin interplay and the thorough examination of the catechin-cell membrane interaction need to be performed.

Pterostilbene Enhances Cytotoxicity and Chemosensitivity in Human Pancreatic Cancer Cells

“…In agreement with this, our results showed that PTE triggered cell cycle arrest and apoptosis-and autophagy-mediated cytotoxicity and inhibited MDR1 expression by regulating the RAGE/PI3K/Akt axis. Although several natural compounds are used in combined therapies against pancreatic cancer such as catechins, resveratrol, and curcumin [38][39][40]. However, few available publications showed that natural compounds directly target MDR1 expression and promote chemosensitivity.…”

Recent Advances in Electrochemotherapy

“…Among many compounds tested, that is, doxorubicin, 36 ruthenium (III) compound, 37 5-fluorouracil, 38 phthalocyanines, 39 photofrin, 40 curcumin, 41 the trans-platinum analogue trans-[PtCl 2 (3-hmpy) 2 ], 42 oxaliplatin, 43 betulinic acid, 44,45 oat b-glucan, 46 cyanine IR-775, 47 catechin, 48 and sodium decahydrodecaborate, 49 only calcium electroporation was extensively studied. [50][51][52][53][54][55][56][57][58] Calcium electroporation effectively causes tumor necrosis, leaving the normal tissue less affected, making it a very promising compound for further clinical evaluation as it does not belong to the antineoplastic drugs, thus precautions related to antineoplastic drugs regarding storage, handling, disposal, and toxicity and mutagenicity are not an issue for calcium electroporation.…”

Investigating the Role of Dahuang in Hepatoma Treatment Using Network Pharmacology, Molecular Docking, and Survival Analysis

“…Catechin has good antitumor effects against gastric cancer, cervical cancer, pancreatic cancer, and lung cancer. However, two of its isomers have different functions [49][50][51][52]. The four isomers of catechin are (+)-catechin, (-)-catechin, (+)-epicatechin, and (-)-epicatechin.…”