The Fc receptor for IgA expression and affinity on lymphocytes and macrophages

Moţa, Gabriela; Nicolae, Mariana; Moraru, I

doi:10.1016/0161-5890(88)90056-9

Cited by 9 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of receptors for IgA on a wide variety of cells suggests an important role for IgA receptor-mediated immunological processes. Different receptors for IgA have been described; the polymeric immunoglobulin receptor (pIgR) [7], which binds to the C2 and C3 domains of the IgA molecule [8], the asiaglycoprotein receptor [9] which binds to the galactose residues present in the C2-, C3-and the hinge region of the immunoglobulin [10], a receptor for the Fc-region of IgA (FcR) on lymphoid cells [11][12][13][14][15][16], and a FcR on myeloid cells [17,18]. On monocytes and neutrophils, the FcR has been defined as a 55-75-kD glycoprotein [17], and as a 70-100-kD glycoprotein on eosinophils [18].…”

Section: Introductionmentioning

confidence: 99%

Transforming growth factor-beta 1 (TGF-β1) down-regulates IgA Fc-receptor (CD89) expression on human monocytes

Reterink

Levarht

Klar-Mohamad

et al. 1996

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYIgA is the predominant immunoglobulin in human secretions and the second most important immunoglobulin in the circulation on a quantitative basis. The clearance of IgA is dependent on the function of at least three types of receptors. One of these receptors recognizes the Fc portion of the IgA molecule, Fc®R, which has been cloned recently. Fc®R, also designated CD89, is found on a number of cells, including human glomerular mesangial cells, and monocytes. In this study we analysed the effect of TGF-¯1, a cytokine with strong immunosuppressive function, on the expression of CD89 on freshly isolated monocytes. We found that TGF-¯1 down-regulates CD89 expression on human peripheral blood monocytes in a dose-dependent fashion. Optimal down-regulation occurred at a concentration of 5 ng/ml. The down-regulation of CD89 by TGF-¯1 is linear in time, with a mean down-regulation of 34 6 13% after 24 h. Also at the mRNA level, CD89 expression was down-regulated by TGF-¯1, suggesting regulation of CD89 at the transcriptional level. Monocytes pre-treated with TGF-¯1 displayed a reduced response to IgA, as measured by IL-6 production by monocytes, in contrast to monocytes pre-treated with medium alone. These results suggest an important role for TGF-¯1 in the regulation of CD89. This down-regulation may have direct consequences for the handling of IgA by human monocytes.

show abstract

Section: Introductionmentioning

confidence: 99%

Transforming growth factor-beta 1 (TGF-β1) down-regulates IgA Fc-receptor (CD89) expression on human monocytes

Reterink

Levarht

Klar-Mohamad

et al. 1996

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…One has to emphasize that a comparison of respiratory secretion to serum transport ratios of pIgA, mIgA and IgG is likely to be influenced to a great extent by differences of the antibodies in extravascular distribution, binding to various cells, etc. The higher transport ratio of mIgA compared to IgG2a observed in our study in the absence of any known specific transport systems could be explained by those factors.The degree of active transport of pIgA into the lower respiratory tract is likely to be an underestimate because the large size of pIgA disfavors its extravascular distribution and on the other hand a relative high affinity of pIgA for alveolar macrophages [32] could decrease the amount of antibody found in bronchoalveolar washings.…”

Section: Discussionmentioning

confidence: 99%

Monoclonal IgA class‐switch variants against bacterial surface antigens: molecular forms and transport into murine respiratory secretions

et al. 1994

View full text Add to dashboard Cite

The present study describes a new model for passive immunization of the respiratory tract with IgA in comparison to other isotypes. Monoclonal IgA-isotype-switch variants were isolated from different IgG-producing hybridoma clones specific for surface epitopes of bacterial respiratory tract pathogens. Analysis of the molecular form of the IgA variants revealed the simultaneous production of monomeric, dimeric and higher polymeric IgA by a single-cell line with predominance of the polymeric forms. The specificities of the IgA variants were identical to the parent IgG antibodies as demonstrated by inhibition experiments. The IgA variant antibodies were separated into monomers and polymers by gel filtration. Intravenous injection of the different molecular forms of IgA and of IgG into mice were used to investigate the transport characteristics of IgA into murine upper and lower respiratory tract secretions by the physiological route in comparison to IgG. Polymeric IgA variant, monomeric IgA variant and IgG were detected in immunologically active form in both nasal secretion and bronchoalveolar fluid as evidenced by binding to their antigens in an enzyme-linked immunosorbent assay. The relative contribution of the specific exogenous monoclonal IgA and monoclonal IgG to total IgA and IgG, respectively, was determined in secretions. Comparison of the secretion to serum transport ratios clearly indicates selective transport of polymeric IgA variant into nasal secretions relative to IgG parent antibody. Molecular and functional characteristics of the IgA variants make them ideal for passive mucosal immunization experiments and identification of protective epitopes in mucosal immunity.

show abstract

“…Normal serum IgA was purified from a pool of human sera, and myeloma IgA from sera of patients, and finally the chromatographic fractions corresponding to a molecular mass of 160 (monomeric) or 320 kDa (dimeric) were collected. Fab § fragments were prepared from sIgA by papain digestion at pH 7.0, followed by repeated gel filtrations on Sephadex G-200 (Pharmacia) as previously described [44]. Anti-CD89 mAb A59 was obtained from Becton Dickinson/Pharmingen (San Diego, CA).…”

Section: Antibodies Flow Cytometry and Other Reagentsmentioning

confidence: 99%

“…Human sIgA was obtained from Sigma, or prepared from a pool of human casein-free colostrums by precipitation with 40% (NH 4 ) 2 SO 4 followed by chromatography on Sepharose 6B column (Pharmacia), and the fraction corresponding to a molecular mass of 400 kDa was collected [44]. Goat or rabbit IgG anti-human sIgA were isolated by ion exchange chromatography on DEAE-Sephacel (Pharmacia, Uppsala, Sweden), and purity was examined by immunoelectrophoresis [44]. Normal serum IgA was purified from a pool of human sera, and myeloma IgA from sera of patients, and finally the chromatographic fractions corresponding to a molecular mass of 160 (monomeric) or 320 kDa (dimeric) were collected.…”

Section: Antibodies Flow Cytometry and Other Reagentsmentioning

confidence: 99%

“…Purified NK cells were incubated with sIgA (0.7 mg/ 5×10 6 cells/ml) for 1 h at 0°C, washed and then treated for 5 min with goat IgG anti-human IgA Ab [44]. After washing, aliquots of 5×10 6 NK cells were incubated for 30 min at 4°C in a lysis buffer (137 mM NaCl, 20 mM Tris pH 7.4, 2 mM Na 2 VO 4 , 10% glycerol, 1% Nonidet-P40, 1 mM PMSF, 10 ?…”

Section: In Vitro Kinase Assaymentioning

confidence: 99%

See 1 more Smart Citation

Human NK cells express Fc receptors for IgA which mediate signal transduction and target cell killing

Moţa¹,

Manciulea²,

Cosma³

et al. 2003

Eur J Immunol

View full text Add to dashboard Cite

show abstract

The Fc receptor for IgA expression and affinity on lymphocytes and macrophages

Cited by 9 publications

References 22 publications

Transforming growth factor-beta 1 (TGF-β1) down-regulates IgA Fc-receptor (CD89) expression on human monocytes

Transforming growth factor-beta 1 (TGF-β1) down-regulates IgA Fc-receptor (CD89) expression on human monocytes

Monoclonal IgA class‐switch variants against bacterial surface antigens: molecular forms and transport into murine respiratory secretions

Human NK cells express Fc receptors for IgA which mediate signal transduction and target cell killing

Contact Info

Product

Resources

About