The Fe‐N‐C Nanozyme with Both Accelerated and Inhibited Biocatalytic Activities Capable of Accessing Drug–Drug Interactions

Abstract

Emerging as a cost‐effective and robust enzyme mimic, nanozymes have drawn increasing attention with broad applications ranging from cancer therapy to biosensing. Developing nanozymes with both accelerated and inhibited biocatalytic properties in a biological context is intriguing to peruse more advanced functions of natural enzymes, but remains challenging, because most nanozymes are lack of enzyme‐like molecular structures. By re‐visiting and engineering the well‐known Fe‐N‐C electrocatalyst that has a heme‐like Fe‐Nx active sites, herein, it is reported that Fe‐N‐C could not only catalyze drug metabolization but also had inhibition behaviors similar to cytochrome P450 (CYP), endowing it a potential replacement of CYP for preliminary evaluation of massive potential chemicals, drug dosing guide, and outcome prediction. In addition, in contrast to electrocatalysts, the highly graphitic framework of Fe‐N‐C may not be obligatory for a competitive CYP‐like activity.

Reversible inhibition of the oxidase-like activity of Fe single-atom nanozymes for drug detection

“… 13 And the active sites of previously reported nanoparticles-based nanozymes are usually indeterminate and dissimilar to that of natural enzymes, which makes the mechanism research challenging. 14 …”

Rh particles in N-doped porous carbon materials derived from ZIF-8 as an efficient bifunctional electrocatalyst for the ORR and HER

“…It also demonstrated that the N-doped carbon matrix has a high degree of graphitization, which provides an excellent conductivity for electron transport in catalytic performance. [14][15][16] It is worth noting that I D /I G increased to a similar value to that at 800 C when the heat-treatment temperature was set at 1000 C, implying that pyrolysis at 1000 C would introduce defects. This result can be ascribed to the fact that Zn would be volatilized at the high temperature of 1000 C. 17 To estimate the stability of the coordination architecture in Rh-ZIF, the thermal behaviour of Rh-ZIF was studied by TGA (Fig.…”

Atomic engineering of single-atom nanozymes for enzyme-like catalysis

“…Meanwhile, Fe-N-C exhibited both accelerated and inhibited cytochrome P450-like behaviors in the drug metabolization based on the enzyme-like central structures. Fe-N-C obtained at 400 C could catalyze the oxidation of 1,4-DPH and showed similar inhibiting interactions with other drugs like cytochrome P450, 111 indicating that Fe-N-C SAzyme could replace cytochrome P450 in the evaluation of potential chemicals, studies of drug-drug interactions, dosage guidance and outcome prediction.…”