The feasibility of citric acid as external carbon source for biological phosphorus removal in a sequencing batch biofilm reactor (SBBR)

Mielcarek, Artur; Rodziewicz, Joanna; Janczukowicz, Wojciech; Thornton, Arthur

doi:10.1016/j.bej.2014.10.001

Cited by 28 publications

(8 citation statements)

References 36 publications

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“…Furthermore, our system uses citrate as the substrate, and the production of citrate is well established in the industry. A. niger produces 360 g/L of citric acid34, resulting in a cheap source of our starting material3536.…”

Section: Discussionmentioning

confidence: 99%

Production of itaconate by whole-cell bioconversion of citrate mediated by expression of multiple cis-aconitate decarboxylase (cadA) genes in Escherichia coli

Kim

Seo

Bhatia

et al. 2017

Sci Rep

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Itaconate, a C5 unsaturated dicarboxylic acid, is an important chemical building block that is used in manufacturing high-value products, such as latex and superabsorbent polymers. Itaconate is produced by fermentation of sugars by the filamentous fungus Aspergillus terreus. However, fermentation by A. terreus involves a long fermentation period and the formation of various byproducts, resulting in high production costs. E. coli has been developed as an alternative for producing itaconate. However, fermentation of glucose gives low conversion yields and low productivity. Here, we report the whole-cell bioconversion of citrate to itaconate with enhanced aconitase and cis-aconitate decarboxylase activities by controlling the expression of multiple cadA genes. In addition, this bioconversion system does not require the use of buffers, which reduces the production cost and the byproducts released during purification. Using this whole-cell bioconversion system, we were able to catalyze the conversion of 319.8 mM of itaconate (41.6 g/L) from 500 mM citrate without any buffer system or additional cofactors, with 64.0% conversion in 19 h and a productivity of 2.19 g/L/h. Our bioconversion system suggests very high productivity for itaconate production.

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Section: Discussionmentioning

confidence: 99%

Production of itaconate by whole-cell bioconversion of citrate mediated by expression of multiple cis-aconitate decarboxylase (cadA) genes in Escherichia coli

Kim

Seo

Bhatia

et al. 2017

Sci Rep

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“…Sequencing batch biofilm reactors are still considered a relatively novel treatment technology (Mielcarek et al, 2015); however, they are growing in reputability. The growth carrier may be fixed, a moving bed or in suspension.…”

Section: Traditional Ebpr Systems and Recent Enhancementsmentioning

confidence: 99%

“…Further, some suggest an additional carbon source or chemical precipitation are needed to achieve low effluent P levels (Pastorelli et al, 1999;Wang et al, 2006). The importance of effective biomass removal-through backwashing or otherwise-and the detrimental impact on EBPR if not effectively implemented, has been reported on a number of occasions (Morgenroth and Wilderer, 1999;Arnz et al, 2001;Mielcarek et al, 2015). This is of importance when considering the requirement for minimal operational maintenance, which is desirable at small and/or remote works.…”

Section: Traditional Ebpr Systems and Recent Enhancementsmentioning

confidence: 99%

A Review of Phosphorus Removal Technologies and Their Applicability to Small-Scale Domestic Wastewater Treatment Systems

Bunce

Ndam

Ofiţeru

et al. 2018

Front. Environ. Sci.

401

204

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The removal of phosphorus (P) from domestic wastewater is primarily to reduce the potential for eutrophication in receiving waters, and is mandated and common in many countries. However, most P-removal technologies have been developed for use at larger wastewater treatment plants that have economies-of-scale, rigorous monitoring, and in-house operating expertise. Smaller treatment plants often do not have these luxuries, which is problematic because there is concern that P releases from small treatment systems may have greater environmental impact than previously believed. Here P-removal technologies are reviewed with the goal of determining which treatment options are amenable to small-scale applications. Significant progress has been made in developing some technologies for small-scale application, namely sorptive media. However, as this review shows, there is a shortage of treatment technologies for P-removal at smaller scales, particularly sustainable and reliable options that demand minimal operating and maintenance expertise or are suited to northern latitudes. In view of emerging regulatory pressure, investment should be made in developing new or adapting existing P-removal technologies, specifically for implementation at small-scale treatment works.

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“…Therefore, it is necessary to add an extra carbon source to balance the nutrition ratio of the biochemical system in constructed wetlands when necessary. Currently, external carbon sources have attracted attention for increasing the COD (Chemical Oxygen Demand)/NO 3 -N ratio of the influent wastewater to enhance the total nitrogen removal efficiency [30][31][32][33][34]. These available organic carbon sources enhance the nitrogen removal efficiency in the wastewater treatment [35,36].…”

Section: Introductionmentioning

confidence: 99%

Methane Emissions Driven by Adding a Gradient of Ethanol as Carbon Source in Integrated Vertical-Flow Constructed Wetlands

Liu¹,

Wang

et al. 2019

Water

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This work aims to investigate the methane emissions from integrated vertical-flow constructed wetlands (IVCWs) when ethanol is added as an external carbon source. In this study, a gradient of ethanol (0, 2, 4, 8, 16 and 32 mmol/L) was added as the carbon source in an IVCW planted with Cyperus alternifolius L. The results showed that the methane emission flux at an ethanol concentration of 32 mmol/L was 32.34 g CH4 m−2 day−1 less than that of the control experiment (0 mmol/L) and that the methane emission flux at an ethanol concentration of 16 mmol/L was 5.53 g CH4 m−2 day−1 less than that at 0 mmol/L. In addition, variations in the water quality driven by the different ethanol concentrations were found, with a redox potential range of −64 mV to +30 mV, a pH range of 6.6–6.9, a chemical oxygen demand (COD) removal rate range of 41% to 78%, and an ammonia nitrogen removal rate range of 59% to 82% after the ethanol addition. With the average CH4-C/TOC (%) value of 35% driven by ethanol, it will be beneficial to understand that CH4-C/TOC can be considered an ecological indicator of anthropogenic methanogenesis from treatment wetlands when driven by carbon sources or carbon loading. It can be concluded that adding ethanol as an external carbon source can not only meet the water quality demand of the IVCW treatment system but also stimulate and increase the average CH4 emissions from IVCWs by 23% compared with the control experiment. This finding indicates that an external carbon source can stimulate more CH4 emissions from IVCWs and shows the importance of carbon sources during sewage treatment processes when considering greenhouse emissions from treated wetlands.

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The feasibility of citric acid as external carbon source for biological phosphorus removal in a sequencing batch biofilm reactor (SBBR)

Cited by 28 publications

References 36 publications

Production of itaconate by whole-cell bioconversion of citrate mediated by expression of multiple cis-aconitate decarboxylase (cadA) genes in Escherichia coli

Production of itaconate by whole-cell bioconversion of citrate mediated by expression of multiple cis-aconitate decarboxylase (cadA) genes in Escherichia coli

A Review of Phosphorus Removal Technologies and Their Applicability to Small-Scale Domestic Wastewater Treatment Systems

Methane Emissions Driven by Adding a Gradient of Ethanol as Carbon Source in Integrated Vertical-Flow Constructed Wetlands

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