The feasibility of the zebrafish embryo as a promising alternative for acute toxicity test using various fish species: A critical review

Su, Tenghui; Lian, Deru; Bai, Yunfei; Wang, Yolina Yu Lin; Zhang, Dainan; Wang, Zhen; You, Jing

doi:10.1016/j.scitotenv.2021.147705

Cited by 35 publications

(20 citation statements)

References 31 publications

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“…24 (0.776, 1.95), 1.98 (0.798, 3.03), and 1.60 (0.705, 2.20), respectively, with their 95% confidence interval covering 1 (Table 3). This indicated there was no sensitivity difference among juvenile G. rarus and juvenile and embryo D. rerio, which is consistent with previous studies (Bai et al, 2021;Belanger et al, 2013;Su et al, 2021) and which could be partially due to the fact that both species belong to the same family (Cyprinidae).…”

Section: Fish Embryo Toxicity Tests (Fet) With D Reriosupporting

confidence: 91%

“…Two approaches were employed to compare the sensitivity across fish species ( G. rarus and D. rerio ) and life stages of D. rerio (juvenile and embryo). First, a chemical toxicity distribution (CTD) model was constructed to compare AFT sensitivity between Chinese rare minnow and zebrafish and the sensitivity of zebrafish AFT and FET (Bai et al, 2021; Su et al, 2021; Wang et al, 2018; Williams et al, 2011). For establishing CTDs, the availability of at least five corresponding toxicity data sets for a given chemical is a prerequisite.…”

Section: Methodsmentioning

confidence: 99%

“…Validation exercises of the OECD testing guideline demonstrated a strong relationship between the fish embryo tests (FETs) and AFTs (OECD, 2012), which was supported by a series of studies (Belanger et al, 2013; Knöbel et al, 2012; Lammer et al, 2009; Ratte & Hammers‐Wirtz, 2003; Rawlings et al, 2019; Selderslaghs et al, 2012). Our recent review revealed that zebrafish FETs could act as a promising alternative for AFTs across several fish species, including G. rarus , yet the results only focused on chemicals from a relatively small dataset available so far (Su et al, 2021). However, fragrance ingredients were seldom included in the FET validation processes and there are limited data on fragrances tested with either D. rerio embryos or juvenile G. rarus .…”

Section: Introductionmentioning

confidence: 99%

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Investigations On the Fish Acute Toxicity of Fragrance Ingredients Involving Chinese Fish Species and Zebrafish Embryos

Zhou

Bai

et al. 2022

Enviro Toxic and Chemistry

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While zebrafish (Danio rerio) have been accepted worldwide for evaluating chemical hazards to aquatic vertebrates, and in some countries it is mandated to generate fish toxicity data using native species, such as Chinese rare minnow (Gobiocypris rarus) in China. This represents an additional regulatory constraint that may cause redundant tests, additional animal uses, and higher costs. Previous studies showed that juvenile G. rarus was more sensitive than zebrafish juveniles and embryos to metals. To better understand the sensitivity of G. rarus to organic chemicals, we selected 29 fragrance ingredients belonging to various chemical classes and with differing physicochemical properties, for which good quality zebrafish acute toxicity data were available and tested them with juvenile G. rarus and embryo D. rerio using the Organisation of Economic Co-operation and Development test guidelines. Chemical toxicity distribution (CTD) and chemical ratio distribution (CRD) models were established to systematically compare the sensitivity between juveniles of G. rarus and D. rerio, as well as between D. rerio embryos and juveniles. The results of the CTD models showed that for tested chemicals, the sensitivity of juvenile G. rarus was similar to that of D. rerio juveniles and embryos. The CRD comparisons revealed that juvenile G. rarus was slightly less sensitive by a factor of ~2 than juvenile D. rerio to ingredients belonging to Verhaar class 3 and Ecological Structure Activity Relationship ester class, while comparable to other chemicals. These comparative experiments demonstrated that fish toxicity data with G. rarus can be submitted for use in chemical registrations outside China, which would avoid repeating animal tests using D. rerio. Meanwhile, the similar sensitivity of zebrafish juveniles and embryos to fragrance ingredients confirmed the suitability of replacing juveniles by zebrafish embryos.

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Section: Fish Embryo Toxicity Tests (Fet) With D Reriosupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Investigations On the Fish Acute Toxicity of Fragrance Ingredients Involving Chinese Fish Species and Zebrafish Embryos

Zhou

Bai

et al. 2022

Enviro Toxic and Chemistry

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“…Due to its small size, a single Zebrafish embryo can be maintained in low fluid volumes for the first six days of development including use of microtiter plates. The permeability of zebrafish embryos is prominent; for example, small chemicals added to fish water permeate into the embryos easily simplifying drug administration and assay processing 36 . Chemical screening can be completed after a few days due to the embryo's rapid development.…”

Section: Discussionmentioning

confidence: 99%

In-Vivo Toxicity of Bioreactor-Grown Biomass And Exopolysaccharides From Malaysian Tiger Milk Mushroom Mycelium For Potential Future Health Applications

Usuldin

Wan-Mohtar

Ilham

et al. 2021

Preprint

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Natural mycelial biomass (MB) and exopolysaccharide (EPS) produced from bioreactor-grown Malaysian Tiger Milk Mushroom Lignosus rhinocerus are considered high-end components due to their high commercial potential value in drug discovery. Both MB (0.16-10 mg/mL) and EPS (0.16-10 mg/mL) extracts were tested for Zebrafish Embryo Toxicity (ZFET) and early development effects on Zebrafish Embryos (ZE) during 24-120 h post-fertilization (HPF) in order to assess toxicity given potential application as a new therapy for treating asthma. Finding revealed that MB was deemed to be safe with an LC50 of 0.77 mg/mL; EPS was shown to be non-toxic (LC50 of 0.41 mg/mL). Neither MB nor EPS delayed hatching nor teratogenic defects in treated ZE at a dose of 2.5 mg/mL. There were no significant changes in the ZE heart rate after MB or EPS treatments with MB (0.16-10 mg/mL: 130 beats/min) and EPS (0.16-10 mg/mL: 140 beats/min), as compared to normal ZE (120-180 beats/min). In addition, mixing of both natural compounds MB and EPS did not affect toxicity using ZFET testing; thus, intimating their safe future use as therapeutic interventions.

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“…Due to its small size, a single Zebrafish embryo can be maintained in low fluid volumes for the first six days of development, including microtiter plates. The permeability of zebrafish embryos is prominent; for example, small chemicals added to fish water permeate the embryos, simplifying drug administration and assay processing 32 . Chemical screening can be completed after a few days due to the embryo's rapid development.…”

Section: Introductionmentioning

confidence: 99%

In vivo toxicity of bioreactor-grown biomass and exopolysaccharides from Malaysian tiger milk mushroom mycelium for potential future health applications

Usuldin

Wan‐Mohtar

Ilham

et al. 2021

Sci Rep

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Natural mycelial biomass (MB) and exopolysaccharides (EPS) of Malaysian tiger milk mushroom Lignosus rhinocerus are considered high-end components due to their high commercial potential value in drug discovery. This study aims to evaluate the toxicity of the mushroom extracts’ generated in a bioreactor using the zebrafish embryo toxicity (ZFET) model assay as a new therapy for treating asthma. Both MB and EPS extracts, at concentrations 0.16–10 mg/mL, were tested for ZFET and early development effects on Zebrafish Embryos (ZE) during 24–120 h post-fertilisation (HPF). Findings revealed that MB was deemed safe with an LC50 of 0.77 mg/mL; the EPS were non-toxic (LC50 of 0.41 mg/mL). Neither MB nor EPS delayed hatching nor teratogenic defects in the treated ZE at a 2.5 mg/mL dose. There were no significant changes in the ZE heart rate after treatments with MB (130 beats/min) and EPS (140 beats/min), compared to that of normal ZE (120–180 beats/min). Mixing both natural compounds MB and EPS did not affect toxicity using ZFET testing; thus, intimating their safe future use as therapeutic interventions. This represents the first study to have used the ZFET assay on MB and EPS extracts of L. rhinocerus for future health applications.

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The feasibility of the zebrafish embryo as a promising alternative for acute toxicity test using various fish species: A critical review

Cited by 35 publications

References 31 publications

Investigations On the Fish Acute Toxicity of Fragrance Ingredients Involving Chinese Fish Species and Zebrafish Embryos

Investigations On the Fish Acute Toxicity of Fragrance Ingredients Involving Chinese Fish Species and Zebrafish Embryos

In-Vivo Toxicity of Bioreactor-Grown Biomass And Exopolysaccharides From Malaysian Tiger Milk Mushroom Mycelium For Potential Future Health Applications

In vivo toxicity of bioreactor-grown biomass and exopolysaccharides from Malaysian tiger milk mushroom mycelium for potential future health applications

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