The feasibility of using primary shrimp hemocyte culture to screen herbal immunostimulants

Huang, Hui Yu; Pan, Luqing; Pan, Shanshan; Song, Mengsi

doi:10.1007/s10499-018-0238-2

Cited by 6 publications

(3 citation statements)

References 43 publications

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“…The total hemocyte count results of our study showed that the P. major extract did not cause a significant change in hemocyte count depending on the dose. The result of our study regarding the total hemocyte count of the P. major extract is consistent with the results of Zubair et al (2012) and Huang et al (2018) .…”

Section: Discussionsupporting

confidence: 92%

“…While the effects of the plant extracts on the total hemocyte count or lysis activity were different between in vitro and in vivo , the PO activity and its effects on the phagocytic activity showed a strong correlation in in vitro and in vivo experimental results ( Huang et al, 2018 ). For this reason, examining the effects of plant extracts on immunity by in vivo studies with model organisms provides healthier results.…”

Section: Discussionmentioning

confidence: 99%

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Immunosuppressive effect of Plantago major on the innate immunity of Galleria mellonella

Kaya

2023

PeerJ

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Greater plantain (Plantago major), a medicinal plant species, is used in folk medicine for the treatment of various diseases in many countries of the world. Different studies have shown that the bioactive components contained in the plant have a dual effect. It was also reported that in vivo and in vitro studies showed different results. The aim of the study was to determine the effects of P. major extract on the hemocyte-mediated and humoral immune responses of the invertebrate model organism Galleria mellonella, which is widely used in immune studies. In the evaluation of these effects, total hemocyte count, encapsulation, melanization, phenoloxidase, superoxide dismutase, catalase, malondialdehyde and total protein parameters were evaluated. The results of the study showed that the total hemocyte count did not change, that the encapsulation responses decreased, that the melanization responses and phenoloxidase activity increased and that the superoxide dismutase activity decreased. As a result, it was determined that high doses of P. major had negative effects on cell-mediated immunity and antioxidant defence and positive effects on melanization. High doses and continuous use of P. major may have negative effects on living things.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Immunosuppressive effect of Plantago major on the innate immunity of Galleria mellonella

Kaya

2023

PeerJ

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“…PRP activation will initiate ProPO activity which will turn proPO into PO molecules (Jeswin et al, 2013). This activity will cause a catalysis in hydroxylation process of mono-phenol to o-diphenol and oxidation of o-diphenol to o-quinones which then leads to the formation of melanin (Huang et al, 2018). Melanin produced from PO activation has a role as an anti-pathogen that will coat pathogens and cells that have been damaged by infection and form a protective barrier for healthy cells (Cerenius & Söderhäll, 2021).…”

Section: Confirmation Of Wssv Infection Status Wssv Infection Status ...mentioning

confidence: 99%

Evaluation of Recombinant Viral Inhibitor Protein for Whiteleg Shrimp Resistance Against White Spot Syndrome Virus

Abdurrahman,

Alimuddin,

Sudrajat

et al. 2023

JFS

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Viral inhibitor protein (VIP) contributes to shrimp resistance against virus infection. VIP can be used as a new strategy to control white spot syndrome virus (WSSV) infection in shrimp culture by inhibiting its action. This study aimed to evaluate the application of VIP on the whiteleg shrimp (Litopenaeus vannamei) to inhibit the WSSV infection. Shrimp at an average body weight of 6.66±0.08 g was injected by recombinant VIP with two different concentrations (1 μg/g and 10 μg/g) and challenged with WSSV. As a positive control was the shrimp not injected with recombinant VIP and challenged with WSSV, and as a negative control was the shrimp not injected with recombinant VIP and not challenged with WSSV. The results showed that the survival rate of shrimp that were injected with recombinant VIP was significantly increased after being challenged with WSSV (P<0.05). The survival and immune response of shrimp that were injected with recombinant VIP were higher than the positive controls (P<0.05). In this study, the application of recombinant VIP can significantly increase the shrimp resistance against WSSV up to 95.65% higher than the positive control.

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Influence of Artemisia annua (Asteraceae) leaf extract on immunity in Galleria mellonella (Lepidoptera: Pyralidae)

Öztürk,

Kaya

2024

Biologia

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The feasibility of using primary shrimp hemocyte culture to screen herbal immunostimulants

Cited by 6 publications

References 43 publications

Immunosuppressive effect of Plantago major on the innate immunity of Galleria mellonella

Immunosuppressive effect of Plantago major on the innate immunity of Galleria mellonella

Evaluation of Recombinant Viral Inhibitor Protein for Whiteleg Shrimp Resistance Against White Spot Syndrome Virus

Influence of Artemisia annua (Asteraceae) leaf extract on immunity in Galleria mellonella (Lepidoptera: Pyralidae)

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