The FeII(citrate) Fenton reaction under physiological conditions

Illés, Erzsébet; Patra, Subrata; Marks, Vered; Mizrahi, Amir; Meyerstein, Dan

doi:10.1016/j.jinorgbio.2020.111018

Cited by 48 publications

(33 citation statements)

References 45 publications

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“…Endogenous oxidative damage to DNA should be mainly attributed to carbonate radical anion, the product of both the Fenton reaction in the intracellular medium and the decomposition of peroxynitrite in the presence of CO 2 . [1][2][3][5][6][7] Hydroxyl radical chemistry with DNA may still be important in radiation damage during tumor treatment with ionizing radiation or from environmental exposure to x-rays or intense UV light. 4 In addition, formation of ROS in highly localized concentrations may permit HO • to exact its nonspecific damage on DNA when oxidative stress is rampant.…”

Section: 10mentioning

confidence: 99%

“…A similar conclusion was reached when chelated Fe II was studied with the ligand being citrate in physiologically relevant concentrations. 6 Both hydroxyl radical and carbonate radical anion are potent one-electron oxidants with reduction potentials of 2.4 and 1.6 V vs. NHE, respectively. The most sensitive base toward oxidation in DNA is guanine (1.3 V vs. NHE), and not surprisingly, oxidation of 2'deoxyguanosine is a common outcome for both of these free radical oxidants.…”

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confidence: 99%

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On the irrelevancy of hydroxyl radical to DNA damage from oxidative stress and implications for epigenetics

Fleming

Burrows

2020

Chem. Soc. Rev.

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Section: 10mentioning

confidence: 99%

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confidence: 99%

On the irrelevancy of hydroxyl radical to DNA damage from oxidative stress and implications for epigenetics

Fleming

Burrows

2020

Chem. Soc. Rev.

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“…It has been shown that it is not a product of the Fenton reaction in the presence of bicarbonate, which is relevant for physiological conditions. Instead, the carbonate radical anion CO 3 •− is the main ROS to be considered [ 9 , 10 ]. Whereas the • OH radical is highly reactive, producing SSBs and base lesions, CO 3 •− is more selective and serves as a one-electron oxidant that generates a guanine radical cation (G +• ) and leads to the subsequent formation of 7,8-dihydro-8-oxoguanine (8-oxoG), as well as spiroiminodihydantoin (Sp) and guanidinehydantoin (Gh) lesions [ 11 , 12 ].…”

Section: Roles Sources and Propagation Of Oxidative Stressmentioning

confidence: 99%

Impact of Oxidative DNA Damage and the Role of DNA Glycosylases in Neurological Dysfunction

Sousa

Luna

et al. 2021

IJMS

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The human brain requires a high rate of oxygen consumption to perform intense metabolic activities, accounting for 20% of total body oxygen consumption. This high oxygen uptake results in the generation of free radicals, including reactive oxygen species (ROS), which, at physiological levels, are beneficial to the proper functioning of fundamental cellular processes. At supraphysiological levels, however, ROS and associated lesions cause detrimental effects in brain cells, commonly observed in several neurodegenerative disorders. In this review, we focus on the impact of oxidative DNA base lesions and the role of DNA glycosylase enzymes repairing these lesions on brain function and disease. Furthermore, we discuss the role of DNA base oxidation as an epigenetic mechanism involved in brain diseases, as well as potential roles of DNA glycosylases in different epigenetic contexts. We provide a detailed overview of the impact of DNA glycosylases on brain metabolism, cognition, inflammation, tissue loss and regeneration, and age-related neurodegenerative diseases based on evidence collected from animal and human models lacking these enzymes, as well as post-mortem studies on patients with neurological disorders.

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“…The most recent reports indicate that in model in vitro conditions, carbonate radicals (CO 3 ٠ ─ ), rather than · OH, are the major product of the Fenton reaction. CO 3 ٠ ─ specifically generates C8 or C5-oxidized G but does not cause direct DNA strand breaks, whereas, the activity of · OH mainly results in sugar-phosphate oxidation products [ 43 – 47 ]. ROS formation in highly localized concentrations, however, may enable ٠ OH to exert damage to DNA, including the formation of 8-oxo-7,8-dihydroguanine (8-oxoG) when oxidative stress is severe [ 43 – 45 , 48 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidative damage and DNA repair in desiccated recalcitrant embryonic axes of Acer pseudoplatanus L.

et al. 2022

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Background Most plants encounter water stress at one or more different stages of their life cycle. The maintenance of genetic stability is the integral component of desiccation tolerance that defines the storage ability and long-term survival of seeds. Embryonic axes of desiccation-sensitive recalcitrant seeds of Acer pseudoplatnus L. were used to investigate the genotoxic effect of desiccation. Alkaline single-cell gel electrophoresis (comet assay) methodology was optimized and used to provide unique insights into the onset and repair of DNA strand breaks and 8-oxo-7,8-dihydroguanine (8-oxoG) formation during progressive steps of desiccation and rehydration. Results The loss of DNA integrity and impairment of damage repair were significant predictors of the viability of embryonic axes. In contrast to the comet assay, automated electrophoresis failed to detect changes in DNA integrity resulting from desiccation. Notably, no significant correlation was observed between hydroxyl radical (٠OH) production and 8-oxoG formation, although the former is regarded to play a major role in guanine oxidation. Conclusions The high-throughput comet assay represents a sensitive tool for monitoring discrete changes in DNA integrity and assessing the viability status in plant germplasm processed for long-term storage.

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The FeII(citrate) Fenton reaction under physiological conditions

Cited by 48 publications

References 45 publications

On the irrelevancy of hydroxyl radical to DNA damage from oxidative stress and implications for epigenetics

On the irrelevancy of hydroxyl radical to DNA damage from oxidative stress and implications for epigenetics

Impact of Oxidative DNA Damage and the Role of DNA Glycosylases in Neurological Dysfunction

Oxidative damage and DNA repair in desiccated recalcitrant embryonic axes of Acer pseudoplatanus L.

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