The fermented non-digestible fraction of common bean (Phaseolus vulgaris L.) triggers cell cycle arrest and apoptosis in human colon adenocarcinoma cells

Abstract

Cancer is a leading cause of death worldwide with colorectal cancer (CRC) ranking as the third contributing to overall cancer mortality. Non-digestible compounds such as dietary fiber have been inversely associated with CRC in epidemiological in vivo and in vitro studies. In order to investigate the effect of fermentation products from a whole non-digestible fraction of common bean versus the short-chain fatty acid (SCFAs) on colon cancer cells, we evaluated the human gut microbiota fermented non-digestible fraction (hgm-FNDF) of cooked common bean (Phaseolus vulgaris L.) cultivar Negro 8025 and a synthetic mixture SCFAs, mimicking their concentration in the lethal concentration 50 (SCFA-LC 50 ) of FNDF (hgm-FNDF-LC 50 ), on the molecular changes in human colon adenocarcinoma cells (HT-29). Total mRNA from hgm-FNDF-LC 50 and SCFA-LC 50 treated HT-29 cells were used to perform qPCR arrays to determine the effect of the treatments on the transcriptional expression of 84 genes related to the p53-pathway. This study showed that both treatments inhibited cell proliferation in accordance with modulating RB1, CDC2, CDC25A, NFKB and E2F genes. Furthermore, we found an association between the induction of apoptosis and the modulation of APAF1, BID, CASP9, FASLG, TNFR10B and BCL2A genes. The results suggest a mechanism of action by which the fermentation of non-digestible compounds of common bean exert a beneficial effect better than the SCFA mixture by modulating the expression of antiproliferative and pro-apoptotic genes in HT-29 cells to a greater extent, supporting previous results on cell behavior, probably due to the participation of other compounds, such as phenolic fatty acids derivatives and biopetides.

Comparison of free amino acids, antioxidants, soluble phenolic acids, cytotoxicity and immunomodulation of fermented mung bean and soybean

“…In their study, the fermentation product was found to selectively inhibit cancer cell proliferation and induced cell death . In addition, fermented common bean ( Phaseolus vulgaris L.) were reported to induced apoptosis and G1 to S phase cell cycle arrest on human colon cancer cells . More specifically, our results have demonstrated that FMB possessed greater apoptosis inducing effect against breast cancer cells than FSB.…”

“…45 In addition, fermented common bean (Phaseolus vulgaris L.) were reported to induced apoptosis and G1 to S phase cell cycle arrest on human colon cancer cells. 46 More specifically, our results have demonstrated that FMB possessed greater apoptosis inducing wileyonlinelibrary.com/jsfa effect against breast cancer cells than FSB. The disparities of the cytotoxic efficacy between studies might be contributed by the conditions of fermentation as well as the extraction process prior to the in vitro studies.…”

Mechanisms associated to apoptosis of cancer cells by phenolic extracts from two canned common beans varieties (Phaseolus vulgarisL.)

“…However in Northern Mexico, Bayo Victoria beans have had traditionally higher preference from consumers, and therefore they are also used by the food industry for processing and marketing. In previous reports, we have indicated that Bayo Victoria beans have antioxidant and anti‐inflammatory activities (Moreno‐Jiménez et al, ), containing phenolic compounds with antiproliferative effects as described also in the literature (Campos‐Vega et al, ; Cruz Bravo et al, ; ). These studies indicate that Phaseolus vulgaris consumption can be recommended for cancer prevention.…”

“…Vergara-Castañeda et al (2010) have determined the chemoprotective effect at early stages of colon cancer (in vivo models). In this context, multiple mechanisms have been suggested, when evaluating common bean effects in cell survival (Cruz-Bravo et al, 2011), cell cycle arrest and apoptosis in human colon adenocarcinoma cells (Cruz-Bravo et al, 2014).…”

Impact of diet on the human intestinal microbiota

“…Propionate participates in the succinate pathway and acrylate pathway [39]. And propionate as well as butyrate can induce apoptosis of human colon carcinoma cells which are infinite proliferous and finally lead to tumours [40]. This discovery may provide a clue to help clean those carcinoma cells in a 'healthy' way.…”