The ferredoxin Rr-HydB is required for the H2-evolving activity of Rr-HydA, a [FeFe]-hydrogenase of Rhodospirillum rubrum

Hydrogen production by water splitting energized by biomass sugars is one of the most promising technologies for distributed green H production. Direct H generation from NADPH, catalysed by an NADPH-dependent, soluble [NiFe]-hydrogenase (SH1) is thermodynamically unfavourable, resulting in slow volumetric productivity. We designed the biomimetic electron transport chain from NADPH to H by the introduction of an oxygen-insensitive electron mediator benzyl viologen (BV) and an enzyme (NADPH rubredoxin oxidoreductase, NROR), catalysing electron transport between NADPH and BV. The H generation rates using this biomimetic chain increased by approximately five-fold compared to those catalysed only by SH1. The peak volumetric H productivity via the in vitro enzymatic pathway comprised of hyperthermophilic glucose 6-phosphate dehydrogenase, 6-phosphogluconolactonase, and 6-phosphogluconate dehydrogenase, NROR, and SH1 was 310 mmol H /L h , the highest rate yet reported. The concept of biomimetic electron transport chains could be applied to both in vitro and in vivo H production biosystems and artificial photosynthesis.

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Exceptionally High Rates of Biological Hydrogen Production by Biomimetic In Vitro Synthetic Enzymatic Pathways

Kim

Adams

et al. 2016

Chemistry A European J

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Metabolic engineering approaches for scale-up of fermentative biohydrogen production–A review

Akaniro,

Oladipo,

Onwujekwe

2024

International Journal of Hydrogen Energy

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The ferredoxin Rr-HydB is required for the H2-evolving activity of Rr-HydA, a [FeFe]-hydrogenase of Rhodospirillum rubrum

Cited by 2 publications

References 63 publications

Exceptionally High Rates of Biological Hydrogen Production by Biomimetic In Vitro Synthetic Enzymatic Pathways

Exceptionally High Rates of Biological Hydrogen Production by Biomimetic In Vitro Synthetic Enzymatic Pathways

Metabolic engineering approaches for scale-up of fermentative biohydrogen production–A review

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