The Fertility of Cryopreserved Deccan Mahseer, Tor khudree (Sykes) Spermatozoa

Basavaraja, N.; Hegde, S.N.; Akash, N.; Udupa, Karthik S

doi:10.33997/j.afs.2002.15.3.001

AFS

2002

DOI: 10.33997/j.afs.2002.15.3.001

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The Fertility of Cryopreserved Deccan Mahseer, Tor khudree (Sykes) Spermatozoa

N. Basavaraja¹,

S.N. Hegde²,

N. Akash³

et al.

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Conservation of freshwater habitats and fishes in the Western Ghats of India

Kumar¹,

Devi

2013

International Zoo Yearbook

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The Western Ghats, India, present a series of challenges and opportunities for novel approaches to conservation. Running parallel to the coast of the Indian Peninsula, the Ghats represent the western edge of the Deccan Plateau and traverse 1400 km over six states, giving rise to rivers that drain over a third of the country. A global biodiversity hotspot with varied topography housing diverse ecological niches, the Western Ghats present significant challenges to, and opportunities for, conservation. Dense human habitation near river systems and dependence on freshwater resources for livelihoods make it difficult to establish protected areas. Freshwater fishes in this region account for c. 40% of the freshwater fishes in India and around 189 species are endemic to the Western Ghats. All the usual threats to the fishes and their habitats are present, and the freshwater fishes of the Western Ghats are of great social and economic importance to the local community. Zoos, aquariums and the general public can play a role in conservation efforts through education and research. Utilizing traditional customs already in place, such as water bodies in the vicinity of temples where no fishing occurs, in conjunction with ecotourism and more modern technologies, such as live gene banks and cryopreservation, may ease the burden on the freshwater habitats and fishes in the region.

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Conservation of freshwater habitats and fishes in the Western Ghats of India

Kumar¹,

Devi

2013

International Zoo Yearbook

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Effect of cryoprotectants, equilibration periods and freezing rates on cryopreservation of spermatozoa of mahseer, Tor khudree (Sykes) and T. putitora (Hamilton)

Patil

Lakra

2005

Aquac Research

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A study was conducted to standardize a protocol for cryopreservation of spermatozoa of the endangered mahseer, Tor khudree (Sykes) and T. putitora (Hamilton). The suitability of the cryoprotectants, dimethyl sulphoxide (DMSO) and glycerol, and the combination of the two were tested. Four equilibration periods and four freezing rates were also tested for their standardization. A combination of 9% DMSO and 11% glycerol gave signi¢cantly higher mean percentage of hatching in both T. khudree (45.59 AE 1.86%) (control 71.08 AE 0.59%) and T. putitora (45.00 AE 1.25%) (control 73.48 AE 1.19%) among the eight di¡erent treatments. Among the four di¡erent equilibration periods tested, the equilibration period of 30 min À1 yielded the highest mean hatching percentage in T. khudree (39.46 AE 1.94%) (control 71.70 AE 0.61%) and T. putitora (38.28 AE 1.06%) (control 73.11 AE 0.82%). Freezing straws at a height of 8 cm above LN 2 surface for 10 min À1 gave higher hatching percentages for both T. khudree (41.75 AE 1.72%) (control 73.99 AE 1.17%) and T. putitora (41.34 AE 2.04%) (control 72.48 AE 1.51%). The study reports the superior performance of the combination of DMSO and glycerol for the ¢rst time.

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Studies on fresh milt parameters and cellular changes during cryopreservation of spermatozoa of Deccan Mahseer, Tor Khudree (Sykes)

Patil

Lakra

Jahageerdar

et al. 2016

Indian Journal of Fisheries

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Studies on the physico-chemical characteristics of seminal plasma, along with ultrastructure and mitochondrial activity of fresh spermatozoa of the endangered Deccan mahseer Tor khudree were undertaken. The ultrastructure and mitochondrial activity of fresh spermatozoa were compared with those of cryopreserved-thawed spermatozoa to understand the nature and extent of cryo-damage. Physico-chemical analyses of fresh milt revealed sperm density of 3.93±0.11 x 10 7 spermatozoa ml -1 , spermatocrit value of 67.08±1.22%, higher K + concentration of 13.16±0.121 mg l -1 , total reducing sugar and total protein concentration of 47.31±0.82 and 19.60±0.66 mg 100 ml -1 respectively. Ultrastructure of the fresh spermatozoa by both scanning (SEM) and transmission electron microscopy (TEM) revealed the spherical head without any acrosomal complex, small mid piece with mitochondria and a long tail. Cross section of tail by TEM revealed typical 9+2 doublet arrangement of the axoneme. Head measured 1.86±0.006 µ in dia with a mid-piece length of 0.53±0.012 µ and tail length of 33.53±0.220 µ. Ultrastructural damages to the spermatozoa following cryopreservation included, loosening of chromatin and disruption of the cytoplasmic membrane as compared to that of fresh spermatozoa. Nitroblue tetrazolium (NBT) assay revealed low levels of activity of the enzymes of the mitochondrial complex in cryopreserved-thawed spermatozoa when compared to fresh spermatozoa indicating damage to the functional integrity of the enzymes of the mitochondrial enzyme complex.

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The Fertility of Cryopreserved Deccan Mahseer, Tor khudree (Sykes) Spermatozoa

Cited by 3 publications

References 0 publications

Conservation of freshwater habitats and fishes in the Western Ghats of India

Conservation of freshwater habitats and fishes in the Western Ghats of India

Effect of cryoprotectants, equilibration periods and freezing rates on cryopreservation of spermatozoa of mahseer, Tor khudree (Sykes) and T. putitora (Hamilton)

Studies on fresh milt parameters and cellular changes during cryopreservation of spermatozoa of Deccan Mahseer, Tor Khudree (Sykes)

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