The fertility of the hybrid lineage derived from female Megalobrama amblycephala× male Culter alburnus

Xiao, Jun; Kang, Xuewei; Xie, Lihua; Qin, Qinbo; He, Zhoulin; Hu, Fangzhou; Chun, Zhang; Zhao, Rurong; Wang, Jun; Luo, Kun; Liu, Yun; Liu, Shaojun

doi:10.1016/j.anireprosci.2014.09.012

Cited by 83 publications

(48 citation statements)

References 14 publications

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“…The water pH range was 7.0–8.5, the dissolved oxygen range was 5.0–8.0 mg/L, and the ammonia–nitrogen concentration was lower than 0.01 mg/L. The sex of each fish was determined by examining a gonad tissue slice, as described by Xiao et al [33]. The ploidy types of these fish were confirmed based on their DNA contents using a flow cytometer (cell counter analyzer; Partec), as described previously [14].…”

Section: Methodsmentioning

confidence: 99%

Different expression patterns of sperm motility-related genes in testis of diploid and tetraploid cyprinid fish†

Zhou

et al. 2017

Biology of Reproduction

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Sperm motility is an important standard to measure the fertility of male. In our previous study, we found that the diploid spermatozoa from allotetraploid hybrid (4nAT) had longer durations of rapid and slow progressive motility than haploid spermatozoa from common carp (COC). In this study, to explore sperm motility-related molecular mechanisms, we compared the testis tissues transcriptomes from 2-year-old male COC and 4nAT. The RNA-seq data revealed that 2985 genes were differentially expressed between COC and 4nAT, including 2216 upregulated and 769 downregulated genes in 4nAT. Some differentially expressed genes, such as tubulin genes, dynein, axonemal, heavy chain(dnah) genes, mitogen-activated protein kinase(mapk) genes, tektin 4, FOX transcription factors, proteasome genes, and ubiquitin carboxyl-terminal hydrolase(uchl) genes, are involved in the regulation of cell division, flagellar and ciliary motility, gene transcription, cytoskeleton, energy metabolism, and the ubiquitin–proteasome system, suggesting that these genes were related to sperm motility of the 4nAT. We confirmed the differential expression of 12 such genes in 4nAT by quantitative PCR. By western blotting, we also confirmed increased expression of Uchl3 in 4nAT testis. In addition, we identified 1915 and 2551 predicted long noncoding RNA (lncRNA) transcripts from testis tissue transcriptomes of COC and 4nAT, respectively. Of these, 1575 lncRNAs were specifically expressed in 4nAT and 939 were specifically expressed in COC. This study provides insights into the transcriptome profile of testis tissues from diploid and tetraploid, which are useful for research on regulatory mechanisms behind sperm motility in male polyploidy.

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Section: Methodsmentioning

confidence: 99%

Different expression patterns of sperm motility-related genes in testis of diploid and tetraploid cyprinid fish†

Zhou

et al. 2017

Biology of Reproduction

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“…Good-quality pictures of the metaphase spreads from 12 individuals were observed under a microscope (Fig. 1)33.…”

Section: Methodsmentioning

confidence: 99%

Homoeologue expression insights into the basis of growth heterosis at the intersection of ploidy and hybridity in Cyprinidae

Ren

Tao

et al. 2016

Sci Rep

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Hybridization and polyploidization are considered important driving forces that form new epigenetic regulations. To study the changing patterns of expression accompanying hybridization and polyploidization, we used RNA-seq and qRT-PCR to investigate global expression and homoeologue expression in diploid and tetraploid hybrids of Carassius auratus red var. (♀) (R) and Cyprinus carpio (♂) (C). By comparing the relative expression levels between the hybrids and their parents, we defined the expression level dominance (ELD) and homoeologue expression bias (HEB) in liver tissue. The results showed that polyploidization contributed to the conversion of homoeologue ELD. In addition, hybridization had more effect on the change in HEB than polyploidization, while polyploidization had more effect on the change of global gene expression than hybridization. Meanwhile, similar expression patterns were found in growth-related genes. The results suggested that hybridization and polyploidization result in differential degrees of maternal HEB in three tissues (liver, muscle and ovary) tested. The results of this study will increase our understanding of the underlying regulation mechanism of rapid growth in diploid hybrids and allotetraploids. The differential degrees of global expression and homoeologue expression contribute to growth heterosis in newly formed hybrids, ensuring the on-going success of allotetraploid speciation.

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“…(♀)×Cyprinus carpio (♂) (F 3 F 24 ) [4], autotetraploid fish lineages derived from Carassius auratus red var. (♀)×Megalobrama amblycephala (♂) [5], diploid fish lineages derived from Megalobrama amblycephala (♀)×Culter alburnus (♂) (F 1 F 3 ) [6], different ploidy-level hybrids of Megalobrama amblycephala Yih×male Xenocypris davidi Bleeke [7] and different ploidy-level hybrids of red crucian carp×copmouth culter [8]. These hybrid progenies can be used to produce improved triploids or diploids [9,10].…”

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confidence: 99%

Genomic variation in the hybrids of white crucian carp and red crucian carp: evidence from ribosomal DNA

Wang

Xiao

Zeng

et al. 2015

Sci. China Life Sci.

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In this study, we conducted a cross of white crucian carp (♀)×red crucian carp (♂) (WR), and characterized the morphology, reproduction and genetics of the progeny. Different from parents, WR with the gray color showed the hybrid morphological traits of both parents. WR possessed normal gonads producing mature eggs or sperm, and exhibited high fertilization rate (90.2%) and high hatchery rate (80.5%), which contributed to produce and enlarge the population. WR with the same DNA content as parents was a diploid fish with 100 chromosomes (2n=100). Amplified ITS of 45S rDNA, in WR the sequences consisting of 884 bp bases of the entire ITS-1 region, 5.8 S region, and entire ITS-2 region. The sequences showed high similarity between WR and its parents and leaned towards male inheritance. In WR, NTS of 5S rDNA consisted of three length types with total 654 bp bases. From sequence analysis of NTS, WR shared 94.2% and 95.1% similarities with their female and male parent, respectively. Sequence analysis of ITS and NTS revealed that there existed recombination and variation in the hybrid progeny, which was the genetic base for adaptation and speciation. In conclusion, we obtained WR from hybridization and it exhibited hybrid traits in morphology and variation in genetic composition showing essential difference with its parents. The obtainment of WR has important significance in fish genetic breeding.white crucian carp, red crucian carp, hybridization, genomic variation Citation:

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The fertility of the hybrid lineage derived from female Megalobrama amblycephala× male Culter alburnus

Cited by 83 publications

References 14 publications

Different expression patterns of sperm motility-related genes in testis of diploid and tetraploid cyprinid fish†

Different expression patterns of sperm motility-related genes in testis of diploid and tetraploid cyprinid fish†

Homoeologue expression insights into the basis of growth heterosis at the intersection of ploidy and hybridity in Cyprinidae

Genomic variation in the hybrids of white crucian carp and red crucian carp: evidence from ribosomal DNA

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