The Feulgen reaction at the electron microscopy level

Biggiogera, Marco

doi:10.4081/ejh.2024.3989

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Applications Of Osmium Ammine1

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2024

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“…The stained DNA then “colonized” the entire nucleolus. Several reviews summarize in depth the wide spectrum of applications of OA in revealing chromatin architecture (Biggiogera et al 1996 ; Derenzini et al 2014 ; Biggiogera 2024 ).…”

Section: Applications Of Osmium Amminementioning

confidence: 99%

A brief history of the Feulgen reaction

Biggiogera,

Cavallo,

Casali

2024

Histochem Cell Biol

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One hundred years ago, Robert Feulgen published a landmark paper in which he described the first method to stain DNA in cells and tissues. Although a century has passed since the discovery by Feulgen and Rossenbeck, the chemical reaction still exerts an important influence in current histochemical studies. Its contribution in diverse fields, spanning from biomedicine to plant biology, has paved the way for the most significant studies that constitute our current knowledge. The possibility to specifically explore the DNA in cell nuclei while quantifying its content makes it a contemporary and timeless method. Indeed, many histocytochemical studies following the 1924 paper have led to a deep understanding of genome organization in general as well as several specific mechanisms (e.g. DNA duplication or tumour pathology) that, nowadays, constitute some of the most fundamental pillars in biological investigations. In this review, we discuss the chemistry and application of the Feulgen reaction to both light and electron microscopy.

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Section: Applications Of Osmium Amminementioning

confidence: 99%