The fine structure of endothelial cells in freeze-fracture preparations

Yamamoto, Kōji; Fujimoto, Sunao; Takeshige, Yoshio

doi:10.1016/s0022-5320(76)80004-4

Cited by 15 publications

(2 citation statements)

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“…The cells are readily identifiable in freeze fracture replicas (Yamamoto et al, 1976), and, because of their thinness, are easily prepared by rapid-freezing techniques. Numerous studies have utilized freeze fracture and transmission electron microscopy to describe the vesicle and caveolae populations in endothelial cells in an effort to gain some understanding of the normal permeability characteristics of this cell layer (Schneeberger and Karnovsky, 1968;Palade et al, 1979;Simionescu et al, 1974;Johansson, 1979;Gil and Silage, 1980;McGuire and Twietmeyer, 1983;Chien et al, 1982;Gil, 1983).…”

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Morphology of rapidly frozen aortic endothelial cells. Glutaraldehyde fixation increases the number of caveolae.

McGuire¹,

Twietmeyer²

1983

Circulation Research

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SUMMARY. Using a Polaron E7200 quick freeze unit, we investigated the distribution of vesicles (caveolae) bound to the plasma membrane of aortic endothelial cells from adult Sprague-Dawley rats. Counts of caveolae from replicas of rapidly frozen, unfixed samples were made and compared with counts from both aldehyde-fixed, conventionally frozen, and rapidly frozen aortic samples. Aldehyde-fixed samples prepared for freeze fracture by either of the freezing methods revealed a significantly greater number of caveolae than did unfixed, rapidly frozen samples. These findings suggest that the relative number of caveolae is artifactually increased by exposure to aldehyde fixatives. We conclude that previous estimates of the rates of pinocytotic activity in vascular endothelial cells may contain substantial errors based on overestimates of caveolae and underestimates of subplasmalemmal 'free ' vesicles. (Circ Res 53: 424-429, 1983)

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mentioning

confidence: 99%

Morphology of rapidly frozen aortic endothelial cells. Glutaraldehyde fixation increases the number of caveolae.

McGuire¹,

Twietmeyer²

1983

Circulation Research

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“…Fenestrated capillaries of several organs have been examined using this method by FRIEDERICI (1968, 1969), MAUI. (1971), SIMIONESCU et al (1974), FUJITA et al (1975), ISHIMURA et al (1976 and YAMAMOTO et al (1976).…”

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confidence: 99%