The fine structure of myofibrils

Rozsa, G.; Szent‐Györgyi, Albert; Wyckoff, Ralph W. G.

doi:10.1016/0014-4827(50)90032-2

Cited by 53 publications

(9 citation statements)

References 10 publications

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“…Hanson and Huxley (9) suggested that the thick filaments were myosin and that the thin filaments were actin. The localization of myosin in the anisotropic bands has been indicated by other studies (10)(11)(12). In our micrographs of cardiac muscle the thin and thick filaments are evident (Fig.…”

Section: Biopsy Proceduressupporting

confidence: 85%

Experimental Needle Biopsy of the Myocardium of Dogs With Particular Reference to Histologic Study by Electron Microscopy

Price

Weiss

Hata

et al. 1955

The Journal of Experimental Medicine

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Our experience with needle biopsy of the heart in dogs indicates that myocardial tissue can be sampled one or more times in each animal with comparative safety. Tamponade, pericarditis, serious arrhythmias, or myocardial infarction due to the interruption of coronary vessels was not observed. Excellent specimens were obtained for critical study by light and electron microscopy. Casten and Marsh (1) have used biochemical techniques to study myocardial tissue obtained in similar fashion. Histochemical methods would also be applicable. Although limited to animal studies at present, the technique may conceivably be adapted to the study of human disease. Myocardial puncture has been carried out (20–22) in patients for the recording of intracardiac pressures and for other diagnostic purposes without apparent harm. Our study of the myocardium of dogs by electron microscopy generally confirms the observations of other workers, except that presence of significant numbers of red blood cells in the extravascular spaces of the heart had not been previously described (and is possibly an artifact). Nevertheless, it is notable that the tissue cells, cellular membranes, and intracellular structures appeared to be intact and undistorted in the tissue specimens which were obtained, fixed, and examined by these methods.

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Section: Biopsy Proceduressupporting

confidence: 85%

Experimental Needle Biopsy of the Myocardium of Dogs With Particular Reference to Histologic Study by Electron Microscopy

Price

Weiss

Hata

et al. 1955

The Journal of Experimental Medicine

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“…This suggests that the A substance may be identical with myosin and the filaments actin. Rozsa et al have reported a similar opinion (5).…”

Section: Discussionsupporting

confidence: 56%

“…1), there is a region of increased density where the H band should be. This conflicts with observations of others (1,3,5) in which this region is described as an area of decreased density. The difference can be explained by assuming that the fixation of intact fibers with formalin at temperatures above 0 ° C., used by others, induces an early stage of contraction which would be represented according to classical histology (8) by slight migration of the material of the A band toward the A-I junction.…”

Section: Discussioncontrasting

confidence: 55%

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Observations by Electron Microscopy on Contraction of Skeletal Myofibrils Induced With Adenosinetriphosphate

Ashley

Porter

Philpott

et al. 1951

Journal of Experimental Medicine

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The literature contains several reports on the fine structure of isolated myofibrils (1)(2)(3)(4)(5). From these it is clear that the extended fibril is composed, in part, of longitudinally arranged filaments which in the dry state are about 200/~. thick and which show nodosities and densities arranged at regular intervals of about 400/~. In the intact fibril these densities are likely to be in perfect phase or register and thus give to the fibril a fine cross-banding quite distinct from the relatively macroscopic striations. There is some evidence (3, 4) that these filaments are arranged along the periphery of the fibril, thus making of it a tubular structure. From electron microscopy the major striations, the A and Z bands, seem to result from concentrations of a non-fibrous, amorphous material. The association, if any, between this and the filaments has not been clarified. In contraction, the major part of the amorphous material within the A band moves into the I and Z band regions. That more attention has not been given the phenomenon is doubtless due to difficulty in controlling contraction of small muscle units and obtaining intermediate stages in the process.Schick and Hass (6) have described the isolation of fibrils from frozen sections of muscle with the aid of tryptic digestion at 0 ° C. When the isolated fibrils were treated with adenosinetriphosphate, the fibrils progressively shortened to approximately 25 per cent of their original lengths. During this shortening, the cross-striations became indistinct and the fibrils broadened. After a time no further contraction was observed. The action was not reversible.More recent studies (7) have indicated that the contraction could be slowed

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“…Hall, Jakus & Schmitt, 1946) that filaments were present, several possibilities were still open. The filaments might be continuous along the whole length of each sarcomere, running between the A band material (Rozsa, Szent-Gy6rgyi & Wyckoff, 1950); they might be highly extensible and attached to the ends of the A band rodlets; they might run between the A band rodlets as far as the beginning of the H band, and there be joined to the rodlets (Hanson & H. E. Huxley, 1953); or they might be more or less inextensible, interdigitating and sliding between 420 MEASUREMENT OF MUSCLE STRIATIONS the A band rodlets, the gaps between the ends of successive sets of these filaments constituting the H bands, whose width would thus vary with sarcomere length. The last of these possibilities appeared the most attractive to us for several reasons.…”

Section: Interpretation Of the Resultsmentioning

confidence: 99%

Measurement of the striations of isolated muscle fibres with the interference microscope

Huxley¹,

Niedergerke²

1958

The Journal of Physiology

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The fine structure of myofibrils

Cited by 53 publications

References 10 publications

Experimental Needle Biopsy of the Myocardium of Dogs With Particular Reference to Histologic Study by Electron Microscopy

Experimental Needle Biopsy of the Myocardium of Dogs With Particular Reference to Histologic Study by Electron Microscopy

Observations by Electron Microscopy on Contraction of Skeletal Myofibrils Induced With Adenosinetriphosphate

Measurement of the striations of isolated muscle fibres with the interference microscope

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