The First Case of Zika Virus Isolated from a Japanese Patient Who Returned to Japan from Fiji in 2016

Taira, Masakatsu; Ogawa, Tomoko; Nishihara, Haruna; Yamamoto, Kojiro; Hotta, Chiemi; Akita, Mamiko; Tajima, Shigeru; Saijo, Masayuki

doi:10.7883/yoken.jjid.2017.042

Cited by 7 publications

(14 citation statements)

References 12 publications

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“…The ZIKV strain ZIKV/Hu/S36/Chiba/2016 strain 38 and the MR766 strain (MR766-NIID, accession no. LC002520), originally isolated from a rhesus monkey in the Zika forest of Uganda in 1947 and maintained in the National Institute of Infectious Diseases (NIID), Japan, were used in this study.…”

Section: Methodsmentioning

confidence: 99%

“…For cloning viruses, the ZIKV/Hu/S36/Chiba/2016-Vero2 solution, which was obtained by inoculating the patient serum into Vero cells and subsequent passage of the supernatant in Vero cells 38 , was diluted with MEM supplemented with 2% FBS (MEM-2% FBS) and then inoculated into Vero cells in 24-well plate at 0.5–1.0 infectious virus particle/well. Seven days after inoculation, the supernatants were recovered and then used to check the plaque size of the clones using a plaque assay as described above.…”

Section: Methodsmentioning

confidence: 99%

“…Viral RNA was isolated from culture supernatants and serum of the Zika patient 38 using High Pure Viral RNA Kit (Roche Diagnostics, IN, USA) and was used for synthesis and amplification of cDNA using a one-step RT-PCR method using PrimeScript II High Fidelity One Step RT-PCR kit (TAKARA Corp., Shiga, Japan). Viral cDNA were amplified by PCR using the primer sets described in Supplementary Table 1 .…”

Section: Methodsmentioning

confidence: 99%

“…Twelve imported cases of ZIKV infections have been reported in 2016 in Japan. Recently, we isolated a ZIKV strain (ZIKV/Hu/S36/Chiba/2016) from serum of a patient, who returned from Fiji to Japan in April 2016 38 . Phylogenetic analysis indicated that the isolate belonged to Asian/American lineage ZIKV and showed the highest homology to the Tonga strain isolated in 2016 38 .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we isolated a ZIKV strain (ZIKV/Hu/S36/Chiba/2016) from serum of a patient, who returned from Fiji to Japan in April 2016 38 . Phylogenetic analysis indicated that the isolate belonged to Asian/American lineage ZIKV and showed the highest homology to the Tonga strain isolated in 2016 38 . During the characterization process of the ZIKV/Hu/S36/Chiba/2016 isolate in vitro , it was revealed that the isolate included at least two variants with different phenotypes.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of large and small-plaque variants in the Zika virus clinical isolate ZIKV/Hu/S36/Chiba/2016

Kato

Tajima

Nakayama

et al. 2017

Sci Rep

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An Asian/American lineage Zika virus (ZIKV) strain ZIKV/Hu/S36/Chiba/2016 formed 2 types in plaque size, large and small. Genomic analysis of the plaque-forming clones obtained from the isolate indicated that the clones forming small plaques commonly had an adenine nucleotide at position 796 (230Gln in the amino acid sequence), while clones forming large plaques had a guanine nucleotide (230Arg) at the same position, suggesting that this position was associated with the difference in plaque size. Growth kinetics of a large-plaque clone was faster than that of a small-plaque clone in Vero cells. Recombinant ZIKV G796A/rZIKV-MR766, which carries a missense G796A mutation, was produced using an infectious molecular clone of the ZIKV MR766 strain rZIKV-MR766/pMW119-CMVP. The plaque size of the G796A mutant was significantly smaller than that of the parental strain. The G796A mutation clearly reduced the growth rate of the parental virus in Vero cells. Furthermore, the G796A mutation also decreased the virulence of the MR766 strain in IFNAR1 knockout mice. These results indicate that the amino acid variation at position 230 in the viral polyprotein, which is located in the M protein sequence, is a molecular determinant for plaque morphology, growth property, and virulence in mice of ZIKV.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Characterization of large and small-plaque variants in the Zika virus clinical isolate ZIKV/Hu/S36/Chiba/2016

Kato

Tajima

Nakayama

et al. 2017

Sci Rep

Self Cite

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Functional activity of antisera against recombinant Zika virus envelope protein subunits expressed in Escherichia coli

Tham

Ooi

Balasubramaniam

et al. 2019

Preprint

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19The global Zika virus (ZIKV) outbreak across continents has been drawing research attentions 20 to researchers and healthcare professionals. It highlights the urgent development of ZIKV 21 vaccines that offer rapid, precise and specific protection to those living in the high-risk regions -22 the tropical and subtropical regions. As a public health priority, there is a progressive 23 development in the discovery of vaccine candidates and design in recent years. Many efforts 24 have been placed in the in vitro development of ZIKV subunits as the vaccine candidate in 25 various protein expression systems, including bacteria, yeast, plant cells, insect cells and 26 mammalian cells. However, due to the lack of knowledge on humoral and cellular immune 27 responses against virus vaccines, a commercialised vaccine against Dengue virus (DENV) has 28 been suspended due to a health scare in Philippines. Moreover, the closely-related DENV and 29 ZIKV has indicated serological cross-reactivity between both viruses. This has led to greater 30 attentions to precautions needed during the design of ZIKV and DENV vaccines. In this study, 31 we pre-selected, synthesised and expressed the domain III of ZIKV envelope protein (namely 32 rEDIII) based on a previously-established report (GenBank: AMC13911.

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Increased growth ability and pathogenicity of American- and Pacific-subtype Zika virus (ZIKV) strains compared with a Southeast Asian-subtype ZIKV strain

et al. 2019

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We investigated the growth properties and virulence in mice of three Zika virus (ZIKV) strains of Asian/American lineage, PRVABC59, ZIKV/Hu/Chiba/S36/2016 (ChibaS36), and ZIKV/Hu/NIID123/2016 (NIID123), belonging to the three distinct subtypes of this lineage. The American-subtype strain, PRVABC59, showed the highest growth potential in vitro, whereas the Southeast Asian-subtype strain, NIID123, showed the lowest proliferative capacity. Moreover, PRVABC59- and NIID123-infected mice showed the highest and lowest viremia levels and infectious virus levels in the testis, respectively, and the rate of damaged testis in PRVABC59-infected mice was higher than in mice infected with the other two strains. Lastly, ZIKV NS1 antigen was detected in the damaged testes of mice infected with PRVABC59 and the Pacific-subtype strain, ChibaS36, at 2 weeks post-inoculation and in the epididymides of PRVABC59-infected mice at 6 weeks post-inoculation. Our results indicate that PRVABC59 and ChibaS36 exhibit increased abilities to grow in vitro and in vivo and to induce testis damage in mice.

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The First Case of Zika Virus Isolated from a Japanese Patient Who Returned to Japan from Fiji in 2016

Cited by 7 publications

References 12 publications

Characterization of large and small-plaque variants in the Zika virus clinical isolate ZIKV/Hu/S36/Chiba/2016

Characterization of large and small-plaque variants in the Zika virus clinical isolate ZIKV/Hu/S36/Chiba/2016

Functional activity of antisera against recombinant Zika virus envelope protein subunits expressed in Escherichia coli

Increased growth ability and pathogenicity of American- and Pacific-subtype Zika virus (ZIKV) strains compared with a Southeast Asian-subtype ZIKV strain

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