The First Chromosomal Characteristics of Nucleolar Organizer Regions and Karyological Analysis of Two Chaetodontid Fishes (Perciformes, Chaetodontidae)

Abstract

The first chromosomal characteristics of nucleolar organizer regions (NORs) and karyological analysis of triangle butterflyfish (Chaetodon triangulum) and yellow butterflyfish (C. andamanensis) from Andaman Sea, Phuket and Phang Nga Province, Thailand, were studied. Kidney cell samples were taken from five male and five female fish. Mitotic chromosome preparations were conducted using standard squash technique as well as taken directly from kidney cells. Metaphase spreads were performed on microscopic slides and then airdried. Conventional and Ag-NOR banding techniques were applied to stain the chromosome. The results showed that the diploid chromosome number of C. triangulum and C. andamanensis was 2n=48, and the fundamental numbers (NFs) were 48 and 52, respectively. Karyotpes were present as 46 large telocentric and two medium telocentric chromosomes in C. triangulum; and 2 large metacentric, 8 large telocentric, 34 medium telocentric, 2 medium acrocentric, and 2 small telocentric chromosomes in C. andamanensis. No strange sized chromosomes related to sex were observed. After the Ag-NOR banding technique, one pair of NORs was observed on the long arm centromeric region of large telocentric chromosome pair 21 in C. triangulum and on the long arm subcentromeric region of the largest metacentric chromosome pair 1 in C. andamanensis. The karyotype formulas are as follows:

A Report on Classical Cytogenetics of Hihgfin Barb Fish, <i>Cyclocheilichthys armatus</i> (Cypriniformes, Cyprinidae)

“…Chromosomes were directly prepared in vivo as follows by Supiwong et al (2013Supiwong et al ( , 2017. The 0.05% colchicine (1 mL to 100 g body weight) was injected into the intramuscular or abdominal cavity of fish and left for 1 h before sacrificing.…”

Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity

“…On the other hand, they are differences known for C. gachua (Hamilton, 1822), C. lucius (Cuvier, 1831), C. striata (Bloch, 1793) ( Cioffi et al 2015 ), Toxotes chatareus (Hamilton, 1822) ( Supiwong et al 2017b ) and Asian swamp eel, Monopterus albus (Zuiew, 1793) ( Supiwong et al 2019 ). The pattern of microsatellite d(GC) 15 repeats in M. wolffii is similar to that of C. lucius ( Cioffi et al 2015 ) and T. chatareus ( Supiwong et al 2017a ). Interestingly, the patterns of microsatellite d(CAT) 10 repeats in M. atrifasciatus , M. mysticetus and M. singaringan are similar to the patterns of the (CA) 15 repeats on chromosomes of other species in the family Bagridae ( Supiwong et al 2013a , 2014b ).…”

“…In fishes, a single NOR carrying chromosome pair is considered as a primitive state ( Milhomem et al 2013 ). Many families such as Chaetodontidae ( Supiwong et al 2017a ), Lutjanidae ( Phimphan et al 2017 ), Notopteridae ( Maneechot et al 2015 ), Scaridae ( Kaewsri et al 2014 ), Serranidae ( Pinthong et al 2013 ), share this character. Also, for fishes the location of NORs in a terminal position, as seen in the studied species, is also considered as a primitive characteristic ( Vitturi et al 1995 ).…”

First Cytogenetic Study of Green Chromis, Chromis viridis (Perciformes, Pomacentridae) by Conventional Staining and Ag-NOR Banding Techniques

“…The cell suspensions were then fixed in Carnoy's fixative solution (ratio 3 methanol: 1 acetic acid). The cell suspensions were dropped on the slide (Getlekha et al, 2017;Kasiroek et al, 2017;Supiwong et al, 2017).…”