The first evaluation of glucose-6-phosphate dehydrogenase deficiency (G6PD) gene mutation in malaria-endemic region at South Central Timor (SCT) district, Eastern Indonesia 2015-2016

Hutagalung, Jontari; Kusnanto, Hari; Supargiyono,; Sadewa, Ahmad Hamim; Satyagraha, Ari Winasti

doi:10.1088/1755-1315/125/1/012016

Cited by 5 publications

(13 citation statements)

References 10 publications

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“…This study was conducted in Jakarta, Indonesia, from 2018 to 2019. Stored DNA samples collected from the previous study (2013)(2014) in the SCT district, Eastern Indonesia, 24 were selected by purposive sampling with background demographic data fulfilling at least one of the following three criteria: 1) asymptomatic malaria (confirmed by PCR), 2) G6PD deficiency (G6PD level < 6.97 U/gHb by Randox test), or 3) anemia (hemoglobin level < 13 g/ dL in males and < 11 g/dL in females). A total of 381 DNA samples were finally included in the analysis (from 156 males and 225 females).…”

Section: Methodsmentioning

confidence: 99%

“…A total of 381 DNA samples were finally included in the analysis (from 156 males and 225 females). The background demographic data from the previous study 24 were analyzed to investigate their association with G6PD variant status.…”

Section: Methodsmentioning

confidence: 99%

“…19 In Eastern Indonesia, the common reported G6PD variants are Vanua Lava (10884T>C), Viangchan (871G>A), Chatham (1003G>A), Kaiping (1388G>A), and Coimbra (592C>T). 21,22,24,25 In a previous report (2013)(2014) from South Central Timor (SCT), Indonesia, three G6PD variants (Vanua Lava, Chatham, and Viangchan) were analyzed. The Vanua Lava variant (10884T>C) was the most common variant (6.5%, 6/92 cases), whereas Chatham (1003G>A) and Viangchan (871G>A) variants were absent.…”

Section: Introductionmentioning

confidence: 99%

“…The Vanua Lava variant (10884T>C) was the most common variant (6.5%, 6/92 cases), whereas Chatham (1003G>A) and Viangchan (871G>A) variants were absent. 24 To enrich the data on G6PD status in SCT, other G6PD variants (Kaiping, Coimbra, Gaohe, Canton, and Mahidol) were subsequently analyzed in this study. For clarity, all of these results are described together.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of Glucose 6-Phosphate Dehydrogenase Variants in Malaria-Endemic Areas of South Central Timor, Eastern Indonesia

Sulistyaningrum

Arlinda

Hutagalung

et al. 2020

The American Journal of Tropical Medicine and Hygiene

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Primaquine is an effective anti-hypnozoite drug for Plasmodium vivax and Plasmodium ovale. However, it can trigger erythrocyte hemolysis in people with glucose 6-phosphate dehydrogenase (G6PD) deficiency. In a previous report from South Central Timor (SCT), Indonesia, we described the prevalence of Vanua Lava, Chatham, and Viangchan variants; in this study, other G6PD variants (Kaiping, Coimbra, Gaohe, Canton, and Mahidol) were subsequently analyzed. For clarity, all of these results are described together. The 381 DNA samples from the previous study during 2013-2014 were analyzed for G6PD variants by using PCR-restriction fragment length polymorphism (RFLP). The prevalence of G6PD deficiency in SCT was 6.3% (24/381 cases), including 4.2% (16/381 cases), 0.5% (2/381 cases), and 1.6% (6/381 cases) for Coimbra, Kaiping, and Vanua Lava variants, respectively. No other variants were found in this population. A significant association was found between ethnicity and the distribution of G6PD Kaiping in female subjects. A positive association was shown between G6PD activity and heterozygous females carrying Coimbra genotype, hemizygous males carrying Vanua Lava, Plasmodium falciparum infection in female subjects, and P. vivax infection in male subjects. Further molecular analysis of heterozygous females, particularly in malaria-endemic areas, is needed for mapping distribution of G6PD deficiency status in Indonesia.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence of Glucose 6-Phosphate Dehydrogenase Variants in Malaria-Endemic Areas of South Central Timor, Eastern Indonesia

Sulistyaningrum

Arlinda

Hutagalung

et al. 2020

The American Journal of Tropical Medicine and Hygiene

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“…Ethic Approval for this study was approved by ethical committee of the Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia, with reference no: KE/FK/85/ EC. Data were collected from August 2014 to September 2014, and the whole blood samples were taken from the previous study of Hutagalung et al [8,9]. The criteria of inclusion were positive malaria from thick and thin blood smears by Giemsa 3-5% and nested PCR [10], more than 14 years of age, and signed a written informed consent.…”

Section: Methodsmentioning

confidence: 99%

The Genotyping of Glucose 6 Phosphate Dehydrogenase deficiency (G6PD-d) in Malaria Endemic South Central Timor, East Nusa Tenggara, Eastern Indonesia

Hutagalung¹,

Soleha²,

Sitorus³

et al. 2019

Endemic Species

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Approximately 18 million people live in malaria-endemic areas with 218,450 reported confirmed cases and 161 reported deaths in Indonesia. Currently, primaquine (PQ), the 8-aminoquinolines, is still the only drug for radical cure and preventing relapse of malaria. However, the individuals with G6PD deficiency (G6PD-d) have risk of hemolysis. Currently, few data of the prevalence of G6PD-d and genotyping are available. This study will provide the prevalence of G6PD-d and the genotyping in malaria cases in South Central Timor (TTS) district. G6PD status was analyzed with quantitative (Randox G6PD test, UK) follow with PCR-RFLP and sequencing to identify the variant of G6PD-d genotyping. Malaria was confirmed by n-PCR (Promega, Madison, USA). A total 64 of 181 individuals with G6PD-d from South Central Timor (TTS) district were analyzed. About 25 of 64 cases of G6PD-d were tested positive for malaria with P. vivax as the dominant species 56% (14/25) and most of the cases were female 73.3% (11/15). Among the 64 G6PD-d the genotyping Vanua Lava (10,883 T>C) WHO classifies G6PD-d genetic variants class II with severe deficiency <10% the enzyme activity were dominant. The variant of Vanua Lava is dominant and the high G6PD-d indicated that screening for G6PD deficiency is necessary.

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Host susceptibility genes of asymptomatic malaria from South Central Timor, Eastern Indonesia

Fitri

Na‐Bangchang²,

Tjitra³

et al. 2022

Parasitol Res

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The first evaluation of glucose-6-phosphate dehydrogenase deficiency (G6PD) gene mutation in malaria-endemic region at South Central Timor (SCT) district, Eastern Indonesia 2015-2016

Cited by 5 publications

References 10 publications

Prevalence of Glucose 6-Phosphate Dehydrogenase Variants in Malaria-Endemic Areas of South Central Timor, Eastern Indonesia

Prevalence of Glucose 6-Phosphate Dehydrogenase Variants in Malaria-Endemic Areas of South Central Timor, Eastern Indonesia

The Genotyping of Glucose 6 Phosphate Dehydrogenase deficiency (G6PD-d) in Malaria Endemic South Central Timor, East Nusa Tenggara, Eastern Indonesia

Host susceptibility genes of asymptomatic malaria from South Central Timor, Eastern Indonesia

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