The first finding of Hassall's corpuscles in the thymi of cultured sturgeons

Šálková, Eva; Flajšhans, Martin

doi:10.17221/247/2015-vetmed

Cited by 3 publications

(11 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The capillaries start from the interlobular arteries, which are more numerous in the cortical region. The capillary lymphatic network is also denser in the cortical area, which has been described by researchers (Goldstein et al, 2016;Salkova et al, 2017).…”

Section: Discussionsupporting

confidence: 57%

Study of anatomical, histological and cytological characteristics of the thymus of lambs

Rahmoun¹,

Lieshchova²,

Chaanbi³

et al. 2020

TAVM

View full text Add to dashboard Cite

Thymus of lambs of the Ouled-Djellal breed from eastern Algeria, aged one to 10 months, were used to study the anatomical, histological and cytological characteristics of these organs. Modern techniques have been used to demonstrate the morphometric parameters and masses of the thymus at different ages, a regression of the morphometric and mass indexes has been visualized according to the age of the lambs. Staining techniques with Hematoxylin and eosin revealed the normal structure of the thymus with its tissue components. The thymus is bilobed. The lobe is surrounded by a thin capsule and subdivided into lobules separated by septae, the cortical and medullary areas were clearly visible, the interlobular blood vessels enter the lobule in all its aspects to continue its way as interlobular blood vessels. From the changes observed in the involution of the thymus according to the age of the animals which occur relatively late, the percentage of the surface found for the capsule was very variable, which increases from the first to the tenth month, the demarcation of the cortex and the medulla beginning during the second month of age of the animal. The interlobular connective tissue (ILC) presents sums, the maximum of which was found in the fourth month of age of the animal, while the minimum was found in the thymus of ninth month lambs Concerning the Hassal corpuscles, their sums are in high extension until the tenth month, rich in T lymphocytes, whose use of immunohistochemistry, the anti-CD 157+ antibody allowed us to visualize to locate them clearly, which invade and form most of the thymus cortex. In conclusion, the significant changes in thymus development in sheep of the Ouled-Djellal breed are similar to those of other species.

show abstract

Section: Discussionsupporting

confidence: 57%

Study of anatomical, histological and cytological characteristics of the thymus of lambs

Rahmoun¹,

Lieshchova²,

Chaanbi³

et al. 2020

TAVM

View full text Add to dashboard Cite

show abstract

“…2014). Fänge (1986) could not find Hassall’s corpuscles in A. transmontanus , whereas Salkova and Flajshans (2016) claimed their first description in sturgeon ( A. brevirostrum, A. ruthenus ). Still, Lange et al .…”

Section: Resultsmentioning

confidence: 98%

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

Höhne

Prokopov

Kuhl

et al. 2021

Reviews in Aquaculture

View full text Add to dashboard Cite

Sturgeon immunity is relevant for basic evolutionary and applied research, including caviar-and meat-producing aquaculture, protection of wild sturgeons and their re-introduction through conservation aquaculture. Starting from a comprehensive overview of immune organs, we discuss pathways of innate and adaptive immune systems in a vertebrate phylogenetic and genomic context. The thymus as a key organ of adaptive immunity in sturgeons requires future molecular studies. Likewise, data on immune functions of sturgeon-specific pericardial and meningeal tissues are largely missing. Integrating immunological and endocrine functions, the sturgeon head kidney resembles that of teleosts. Recently identified pattern recognition receptors in sturgeon require research on downstream regulation. We review first acipenseriform data on Toll-like receptors (TLRs), type I transmembrane glycoproteins expressed in membranes and endosomes, initiating inflammation and host defence by molecular pattern-induced activation. Retinoic acid-inducible gene-I-like (RIG-like) receptors of sturgeons present RNA and key sensors of virus infections in most cell types. Sturgeons and teleosts share major components of the adaptive immune system, including B cells, immunoglobulins, major histocompatibility complex and the adaptive cellular response by T cells. The ontogeny of the sturgeon innate and onset of adaptive immune genes in different organs remain understudied. In a genomics perspective, our new data on 100 key immune genes exemplify a multitude of evolutionary trajectories after the sturgeon-specific genome duplication, where some single-copy genes contrast with many duplications, allowing tissue specialization, sub-functionalization or both. Our preliminary conclusion should be tested by future evolutionary bioinformatics, involving all >1000 immunity genes. This knowledge update about the acipenseriform immune system identifies several important research gaps and presents a basis for future applications.

show abstract

“…the surface of the thymus is covered with a fine fibrous capsule. The thymus is lobulated and consists of an outer cortex and the inner medulla, with a diverse range of cell types within its microenvironment (Salkova and Flajshans 2016). The epithelial cells constitute a fine epithelial intra-thymic network (Mohammad et al 2007;Gradil et al 2014), while closely packed lymphocytes dominate the cortex.…”

mentioning

confidence: 99%

“…The epithelial cells constitute a fine epithelial intra-thymic network (Mohammad et al 2007;Gradil et al 2014), while closely packed lymphocytes dominate the cortex. The medulla consists of sparsely organised lymphocytes, macrophages, reticular cells, fibroblasts and dispersedly distributed Hassall's corpuscles (Bowden et al 2005;Petrie-Hanson and Peterman 2005;Peterman and Petrie-Hanson 2006;Salkova and Flajshans 2016).…”

mentioning

confidence: 99%

“…The thymus is considered as the primary lymphoid organ in sturgeons, Acipenseridae (Petrie-Hanson and Peterman 2005;Peterman and Petrie-Hanson 2006). The thymus is a paired lymphoepithelial organ situated on the upper inside edge of the operculum in close proximity to the branchial cavity and pharyngeal epithelium (Bowden et al 2005;Salkova and Flajshans 2016). Thymi persist until the fish reach sexual maturity, later the thymus undergoes age-related atrophy.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus)

Šálková¹,

Flajšhans²,

Steinbach³

2020

Vet. Med. - Czech

View full text Add to dashboard Cite

In this study, we describe the immunohistochemical characterisation of the thymus, the main lymphoid organ, in sturgeon. The wide range cytokeratin, vimentin, S-100 protein, LCA (CD45) and CD3 were selected as the immunohistochemical markers to map the thymus in juvenile sterlet (Acipenser ruthenus). The epithelial cells and Hassall’s corpuscles were labelled with a wide range cytokeratin. The fibroblasts and connective tissue within the thin fibrous capsule on the thymic surface expressed vimentin positivity. The stromal reticular cells were S-100 protein positive. The Leukocyte Common Antigen LCA (CD45) was negative on the thymic lymphocytes. The CD3 was negative on the thymic lymphocytes with cross-reactivity on the non-targeted structures. In conclusion, the commercially available antibodies against the wide range cytokeratin, vimentin and S-100 protein can be used to differentiate components of the sturgeon thymus, while the LCA (CD45) and CD3 application failed. We suggest that further studies are needed to generate fish specific antibodies.

show abstract

The first finding of Hassall's corpuscles in the thymi of cultured sturgeons

Cited by 3 publications

References 9 publications

Study of anatomical, histological and cytological characteristics of the thymus of lambs

Study of anatomical, histological and cytological characteristics of the thymus of lambs

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus)

Contact Info

Product

Resources

About

The first finding of Hassall's corpuscles in the thymi of cultured sturgeons

Cited by 3 publications

References 9 publications

Study of anatomical, histological and cytological characteristics of the thymus of lambs

Study of anatomical, histological and cytological characteristics of the thymus of lambs

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus)

Contact Info

Product

Resources

About

Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus)