The first mitochondrial genome of scale insects (Hemiptera: Coccoidea)

Abstract

Here, we report the first mitochondrial genome of scale insects sequenced from Ceroplastes japonicus (Hemiptera: Coccidae). The genome has a circular genome of 14,979 bp in length, with a high A þ T content of 85.15%. Twelve protein-coding genes (excluding atp8), 13 tRNA, and 2 rRNA genes were detected and annotated using the MITOS web server. The absence of atp8 and some tRNAs might indicate possible novel structures or loss of genes.

Peer Review #1 of "The highly rearranged mitochondrial genomes of three economically important scale insects and the mitochondrial phylogeny of Coccoidea (Hemiptera: Sternorrhyncha) (v0.1)"

“…The complete mitogenomes of U. yanonensis, P. citri and C. rubens were all typical double-strand circular molecules with a length of 15,220 bp, 15,549 bp and 15,387 bp, respectively ( Fig. 1), which were similar to other mitogenomes of Coccoidea (Deng et al, 2019;Lu et al, 2020). The standard set of 37 genes (13 PCGs, tRNA genes and two rRNA genes) were all found in the mitogenome of U. yanonensis (Table 2), whereas trnV was lost in P. citri (Table 3); C. rubens lacked five tRNA genes, trnC, trnR, trnS2, trnL1 and trnV (Table 4).…”

“…The mitochondrial genes of U. yanonensis, P. citri and C. rubens were highly rearranged, being different from the two sequenced scale insects, C. japonicus and S. coffeae (Deng et al, 2019;Lu et al, 2020). When compared with D. yakuba, U. yanonensis and P. citri both showed a conserved…”

“…yanonensis and P. citri and COX2 of C. rubens ended with an incomplete stop codon T. In the previously sequenced scale insect, C. japonicus, COX2 also ended with an incomplete T (Deng et al, 2019). The relative synonymous codon usage (RSCU) values were calculated for the three mitogenomes (Fig.…”

“…A+T content of the two CRs was 85.4% and 88.4%, respectively, higher than U. yanonensis and P. citri. The CR of C. japonicus and S. coffeae was 507 bp and 1454 bp, respectively, indicating the highly variable length of CRs in scale insects (Deng et al, 2019;Lu et al, 2020).…”

“…Mitochondrial genome (mitogenome) usually contains a typical set of 37 genes: 13 protein-coding genes (PCG), 22 transfer RNA genes (tRNA), two ribosomal RNA genes (rRNA) and a non-coding control region (CR) and has become one of the most popular molecules used in insect phylogenetic studies (Cameron, 2014). Recently, Deng et al (2019) and Lu et al (2020) respectively sequenced PeerJ reviewing PDF | (2020:07:50601:1:2:NEW 11 Aug 2020)…”