The first molecular detection of Blastocystis subtypes in human faecal samples from Ibague, Colombia

Potes-Morales, Caterine; Osorio-Delgado, Laura A.; Carranza, Julio César; Vallejo, Gustavo Adolfo

doi:10.1016/j.parepi.2020.e00132

Cited by 9 publications

(24 citation statements)

References 16 publications

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“…is one of the most common parasites inhabiting the human colon and encountered in human feaces which has aroused scientific attention due to great controversy about its biology. It is currently considered the most commonly [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

An Association Between Blastocystis Subtypes and Colorectal Cancer Patients: A Significant Different Profile from Non-cancer Individuals

et al. 2022

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Purpose Blastocystis is a common enteric human parasite of non-conclusive pathogenicity which may be determined by subtype (ST) variation. Colorectal cancer (CRC) is considered one of the primary causes of cancer mortality. Blastocystis ST7 has been shown to reduce beneficial intestinal microbiota and may exacerbate CRC. This study assessed the possible association between Blastocystis STs and CRC in comparison to non-cancer patients. Material and Methods A total of 200 fecal samples were obtained from CRC (100) and non-CRC (100) individuals attending Beni-Suef University Hospital, Egypt. Blastocystis was searched for in all samples using microscopy and culturing. Positive subculture samples were genetically sequenced and subtyped using conventional polymerase chain reaction (PCR). Blastocystis STs were determined by sequencing and a phylogenetic tree was created. Related patient characteristics and tumor stages were analyzed for association with presence of Blastocystis . Results Blastocystis was identified in 52% and 42% of CRC and non-cancer individuals, respectively. ST1, 2, and 3 were isolated from both cancer and non-cancer individuals; however, for the first time, ST7 was only isolated from CRC stool samples with significant association. Associated patient characteristics were evaluated as predictors. Conclusion Blastocystosis is highly prevalent in CRC patients, predominantly in the latest CRC grades and stages. To the best of our knowledge, this is the first study to report the identification of Blastocystis ST7 in CRC patients. To determine whether certain STs of Blastocystis are associated with CRC would require further research, including the role played by gut microbiota.

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Section: Introductionmentioning

confidence: 99%

An Association Between Blastocystis Subtypes and Colorectal Cancer Patients: A Significant Different Profile from Non-cancer Individuals

et al. 2022

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“…The eligibility of 229 full-text articles that passed the title and abstract screening phase was assessed based on preset inclusion and exclusion criteria. Finally, 36 articles [ 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 ] were included in this systematic review.…”

Section: Resultsmentioning

confidence: 99%

“…Figure 2 shows the eighteen articles published in Q1 SJR journals [ 50 , 51 , 52 , 54 , 55 , 61 , 63 , 64 , 65 , 70 , 71 , 75 , 76 , 77 , 79 , 80 , 81 , 82 ], the fifteen articles in Q2 SJR journals [ 57 , 58 , 59 , 60 , 62 , 66 , 67 , 68 , 69 , 72 , 73 , 74 , 83 , 84 , 85 ], and the three articles found in Q3 SJR journals [ 53 , 56 , 78 ].…”

Section: Resultsmentioning

confidence: 99%

“…Based on the JBI score rating system tool, thirty-three articles were considered as high-quality scientific papers [ 50 , 51 , 52 , 54 , 55 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 79 , 80 , 81 , 82 , 83 , 84 , 85 ] and only three were of moderate quality [ 53 , 56 , 78 ]. The studies were reported from Argentina [ 50 , 51 ], Bolivia [ 52 , 53 , 54 ], Brazil [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ,…”

Section: Resultsmentioning

confidence: 99%

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Molecular Prevalence and Subtypes Distribution of Blastocystis spp. in Humans of Latin America: A Systematic Review

Fusaro,

Bernal,

Baldiris-Ávila

et al. 2024

TropicalMed

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Blastocystis spp. are among the few enteric parasites with a prevalence that can reach up to approximately 80% in communities of developing countries. This systematic review updates and summarizes available literature on the molecular prevalence and subtype distribution of Blastocystis spp. in Latin American people. This work follows the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. The literature revised covers from 1 January 2015 to 6 October 2023 in seven different scientific databases, and the material was selected through inclusion and exclusion criteria. According to data found in the 36 selected articles, the prevalence of Blastocystis spp. in Latin America ranged between 5.8% (Bolivian rural communities) and 94.0% (Colombian general public). Generally, genomic DNA was extracted from approximately 200 mg fecal sediments using commercial kits, such as the QIAamp Stool Mini Kit (QIAGEN, Hilden, Germany) or the Norgen Stool DNA Isolation Kit (Norgen Biotek Corporation, Thorold, ON, Canada). Subtype-specific primers (such as the couple of primers BhRDr–RD5) developed from unique sequences of the SSU rRNA gene were applied to Blastocystis subtyping. Ten specific subtypes (STs) were found as well as various mixed infections, and the most circulating Blastocystis STs were in the order ST3, ST1, ST2, and ST4. The most recent data about Blastocystis spp. molecular epidemiology and the STs in communities of Latin America are limited to studies from specific countries. Novel scientific data from the other countries are required to obtain a complete picture and truly understand the distribution and prevalence of Blastocystis spp. and the STs.

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“…Regarding subtypes of Blastocystis sp., our results are consistent with previous reports for Colombia and the Americas ( Jiménez et al, 2019 ). Few studies have described subtypes circulating in humans and animals in Colombia; however, they agree that ST1, ST2 and ST3 subtypes are the most frequent ( Ramírez et al, 2016 ; Ramirez et al, 2014 ; Higuera et al, 2020a ; Sánchez et al, 2017 ; Villamizar et al, 2019 ; Potes-Morales et al, 2020 ). In this study, one sample from a control and one sample from a SpA patient were clustered with ST6, which is not frequent in the Americas.…”

Section: Discussionmentioning

confidence: 99%

Frequency and distribution of Blastocystis sp. subtypes in patients with spondyloarthritis in Bogotá, Colombia

Hernández

Morales

Chaparro-Olaya

et al. 2021

Parasite Epidemiology and Control

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Although Blastocystis sp. is one of the most prevalent intestinal parasites worldwide, its role as a pathogen remains unclear. The use of molecular techniques to assess the genetic heterogeneity of Blastocystis sp. has become important to understand its function in some intestinal pathologies and if it is a key component of intestinal microbiota. Spondyloarthritis is a group of immune-mediated autoinflammatory diseases in which microbial dysbiosis in the gut (including parasites, bacteria and fungi) and intestinal inflammation are common features apparently associated with the pathophysiology of these disorders. This study included 74 patients diagnosed with spondyloarthritis and 57 systemically healthy individuals (included as controls), who were screened for intestinal parasites. Blastocystis sp. was detected in 68% and 73% of the patients with spondyloarthritis and controls, respectively. In faecal samples positive for Blastocystis sp., an 18S rRNA gene fragment of Blastocystis sp. was amplified and sequenced to identify their genetic sub-types. Patients with spondyloarthritis showed similar frequencies of ST1, ST2 and ST3 subtypes of Blastocystis sp. (30% each). The same subtypes were observed in controls, wherein almost 60% of the samples showed ST3. In addition, ST6 was found only in one sample from each group. ST1 subtype showed the greatest genetic variability. Although the same subtypes were detected in both patients with spondyloarthritis and controls, subtype prevalence studies conducted in Colombia indicate an association between ST3 and individuals with irritable bowel syndrome. This opens an interesting research avenue to further study of the epidemiology of Blastocystis sp. and its possible relationship with intestinal conditions in immunocompromised patients.

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The first molecular detection of Blastocystis subtypes in human faecal samples from Ibague, Colombia

Cited by 9 publications

References 16 publications

An Association Between Blastocystis Subtypes and Colorectal Cancer Patients: A Significant Different Profile from Non-cancer Individuals

An Association Between Blastocystis Subtypes and Colorectal Cancer Patients: A Significant Different Profile from Non-cancer Individuals

Molecular Prevalence and Subtypes Distribution of Blastocystis spp. in Humans of Latin America: A Systematic Review

Frequency and distribution of Blastocystis sp. subtypes in patients with spondyloarthritis in Bogotá, Colombia

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