The First Pituitary Proteome Landscape From Matched Anterior and Posterior Lobes for a Better Understanding of the Pituitary Gland

Banerjee, Arghya; Biswas, Deepatarup; Barpanda, Abhilash; Halder, Ankit; Sibal, Shamira; Kattimani, Rohit; Shah, Abhidha; Mahadevan, Anita; Goel, Atul; Srivastava, Sanjeeva

doi:10.1016/j.mcpro.2022.100478

Cited by 6 publications

(3 citation statements)

References 60 publications

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“…PRM method development and optimization of target proteins were performed using Skyline (version 19.1) with a method, duration of 60 min ( 26 ). For the MS OT mode, the resolution was 120,000; the scan range was 400 to 1000 ( m/z ); and the maximum injection time was 50 ms.…”

Section: Methodsmentioning

confidence: 99%

In-Depth Serum Proteomics Reveals the Trajectory of Hallmarks of Cancer in Hepatitis B Virus–Related Liver Diseases

Xu¹,

Xu²,

Yin³

et al. 2023

Molecular & Cellular Proteomics

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Section: Methodsmentioning

confidence: 99%

In-Depth Serum Proteomics Reveals the Trajectory of Hallmarks of Cancer in Hepatitis B Virus–Related Liver Diseases

Xu¹,

Xu²,

Yin³

et al. 2023

Molecular & Cellular Proteomics

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“…Following an overnight incubation of 16 h at 37 • C, the digests were concentrated through vacuum drying and reconstituted in 0.1% (v/v) formic acid (FA). Desalting of the peptides was performed using in-house C18 stage tips, as mentioned in our previous study [41]. The desalted peptides were subsequently dried and reconstituted in 0.1% (v/v) FA.…”

Section: Sample Preparation For Targeted Proteomics Analysismentioning

confidence: 99%

Integrated Meta-Omics Analysis Unveils the Pathways Modulating Tumorigenesis and Proliferation in High-Grade Meningioma

Biswas,

Halder,

Barpanda

et al. 2023

Cells

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Meningioma, a primary brain tumor, is commonly encountered and accounts for 39% of overall CNS tumors. Despite significant progress in clinical research, conventional surgical and clinical interventions remain the primary treatment options for meningioma. Several proteomics and transcriptomics studies have identified potential markers and altered biological pathways; however, comprehensive exploration and data integration can help to achieve an in-depth understanding of the altered pathobiology. This study applied integrated meta-analysis strategies to proteomic and transcriptomic datasets comprising 48 tissue samples, identifying around 1832 common genes/proteins to explore the underlying mechanism in high-grade meningioma tumorigenesis. The in silico pathway analysis indicated the roles of extracellular matrix organization (EMO) and integrin binding cascades in regulating the apoptosis, angiogenesis, and proliferation responsible for the pathobiology. Subsequently, the expression of pathway components was validated in an independent cohort of 32 fresh frozen tissue samples using multiple reaction monitoring (MRM), confirming their expression in high-grade meningioma. Furthermore, proteome-level changes in EMO and integrin cell surface interactions were investigated in a high-grade meningioma (IOMM-Lee) cell line by inhibiting integrin-linked kinase (ILK). Inhibition of ILK by administrating Cpd22 demonstrated an anti-proliferative effect, inducing apoptosis and downregulating proteins associated with proliferation and metastasis, which provides mechanistic insight into the disease pathophysiology.

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“…This special issue of Molecular and Cellular Proteomics presents a wide range of primary research, reviews, and perspectives pertaining to clinical proteomics. The expanding scope of proteomics applications is reflected in the spectrum of disease foci represented, including neurodegenerative diseases ( 1 , 2 , 3 , 4 ), infectious disease ( 5 , 6 ), endocrinology ( 7 , 8 ), cardiovascular disease ( 9 ), inflammatory disease ( 10 ), kidney disease ( 11 ), and cancer ( 12 , 13 , 14 , 15 ), as well as in the burgeoning areas of health monitoring and exercise ( 16 , 17 ).…”

mentioning

confidence: 99%

Clinical Proteomics: A Promise Becoming Reality

Gillette,

Jimenez,

Carr

2024

Molecular & Cellular Proteomics

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The First Pituitary Proteome Landscape From Matched Anterior and Posterior Lobes for a Better Understanding of the Pituitary Gland

Cited by 6 publications

References 60 publications

In-Depth Serum Proteomics Reveals the Trajectory of Hallmarks of Cancer in Hepatitis B Virus–Related Liver Diseases

In-Depth Serum Proteomics Reveals the Trajectory of Hallmarks of Cancer in Hepatitis B Virus–Related Liver Diseases

Integrated Meta-Omics Analysis Unveils the Pathways Modulating Tumorigenesis and Proliferation in High-Grade Meningioma

Clinical Proteomics: A Promise Becoming Reality

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