The First Report of Cerebral Nocardiosis Caused by Nocardia terpenica Together With Exiguobacterium profundum Bacteremia

Hu, Naibo; Zou, Wei; Cai, Qingming; Liu, Yanling; Chen, Kaisen; Li, Ming; Tan, Yongming; Zhu, Qing; Zeng, Lingbing

doi:10.5812/jjm.69604

Cited by 7 publications

(8 citation statements)

References 16 publications

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“…To our knowledge, there are no case reports describing any clinical relevance of this genus in animals. In human medicine, these bacteria have only rarely been described in conjunction with clinical afflictions [ 42 , 43 ]. Their ability to affect human skin has been discussed controversially [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Challenges in microbiological identification of aerobic bacteria isolated from the skin of reptiles

et al. 2020

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Introduction Bacterial pathogens are often involved in dermatitis in reptiles. Exact identification of reptilespecific but otherwise uncommon bacterial species may be challenging. However, identification is crucial to evaluate the importance of the detected bacterial species. Objective The aim of this study was to assess the number of aerobic bacterial isolates cultured from skin-derived samples of reptiles which were not reliably identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS), and to determine their identity. Material and methods Routine bacterial diagnostics were performed on 235 skin samples, and 417 bacterial isolates were analysed by MALDI-TOF MS. The isolates were grouped into categories based on their first score: category I (� 2.00), category II (� 1.70 and < 2.00), and category III (< 1.70). Isolates from category III were further investigated by 16S rRNA gene sequencing and the following criteria were applied: query cover 100%, e-value rounded to 0.0 and sequence identity (%) > 98.00% for genus identification, and > 99.00% for species identification. Results The majority of bacterial isolates were in category I (85.1%) or category II (8.4%). In category III (6.5%) results achieved at first by MALDI-TOF MS corresponded to the results of the molecular analysis in 8.0% of isolates at the species level and in 24.0% at the genus level. Bacterial isolates classified as category III were heterogenic in genus (e.g. Chryseobacterium, Devriesea, Pseudomonas, Staphylococcus, Uruburuella), and some have only been described in reptiles so far.

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Section: Discussionmentioning

confidence: 99%

Challenges in microbiological identification of aerobic bacteria isolated from the skin of reptiles

et al. 2020

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“…Among the frequent sites affected by systemic spread, the Central nervous system (CNS) is the most common with 25-45%, [9,13] and can manifest as a single brain abscess, spread to the brain or spinal cord, diffuse encephalitis, and meningitis that simulates neoplasm, vasculitis and infarction [5,16,18]. Nocardia spp.…”

Section: Literature Reviewmentioning

confidence: 99%

“…The genus Nocardia is composed of 85 species. Amongst them, the species whose produce invasive infections with more frequency are N. asteroides, N. farcinica, N. nova, and N. abscessus [12,13,14]. There is a strong association between chronic glucocorticoid therapy and nocardiosis [6,16].…”

Section: Literature Reviewmentioning

confidence: 99%

Brain Abscess Due To Asian Nocardia in a 63 Years-Old Patient with Adult Still´S Disease: A Rare Case Report and Literature Review

2021

J Psychol Neurosci

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Cerebral nocardiosis is a rare and opportunistic pathology caused by a gram-positive, aerobic bacterium of the order of Actinomycetes called Nocardia spp. It appears in patients with immunodeficiencies affecting cellular immunity. Nocardia can affect an organ (lungs in 75% of cases) or manifest itself as a disseminated infection (central nervous system in 25% of cases). Here we present a rare case of 63-years-old patient immunocompromised by monoclonal biologic treatment for Adult-Onset Still disease (AOSD), who subsequently presents fever, signs of motor focality and resonance imaging findings which show supra and infratentorial lesions. The patient is managed in intensive therapy for sensory impairment, receiving standardized antibiotic scheme, getting through to complicated evolution. Cerebral nocardiosis is a rare infectious disease that requires an early diagnostic study and its respective treatment.

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“…Interestingly, we isolated a new nocardial strain from a bacteriemia and CNS-infected patient, N. terpenica NC_YFY_NT001. 25 N. terpenica was first identified in 2007 in a patient suffering from pulmonary nocardiosis. 26 Therefore, it was the first example of N. terpenica causing CNS infection, and the patient eventually recovered.…”

Section: Introductionmentioning

confidence: 99%

“… 26 Therefore, it was the first example of N. terpenica causing CNS infection, and the patient eventually recovered. 25 The experimentally verified virulence factors including genes encoding catalase, cell-wall lipids, superoxide dismutase, hydrolases, phospholipase C, hemolysin (toxic proteins), lipases and proteases have been identified in Nocardia spp . ( Nocardia brasiliensis, Nocardia farcinica, Nocardia cyriacigeorgica ).…”

Section: Introductionmentioning

confidence: 99%

A Complete Genome of Nocardia terpenica NC_YFY_NT001 and Pan-Genomic Analysis Based on Different Sources of Nocardia spp. Isolates Reveal Possibly Host-Related Virulence Factors

Cai¹,

Huang²,

Zhou³

et al. 2022

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Objective We aimed to identify the possible virulence genes associated with Nocardia NC_YFY_NT001 isolated by ourselves and other Nocardia spp . Methods The genome of Nocardia terpenica NC_YFY_NT001 was completed by using PacBio and Illumina platforms. A pan-genomic analysis was applied to selected complete Nocardia genomes. Results Nocardia terpenica NC_YFY_NT001 can cause healthy mice death by tail intravenous injection. The genome of NT001 has one circular chromosome 8,850,000 bp and one circular plasmid 70,000 bp with ~68% GC content. The chromosome and plasmid encode 7914 and 80 proteins, respectively. Furthermore, a pan-genomic analysis showed a total of 45,825 gene clusters, then 304 core, 21,045 shell and 24,476 cloud gene clusters were classified using specific parameters. In addition, we found that catalases were more abundant in human isolates. Furthermore, we also found no significant differences in the MCE proteins between different strains from different sources. The pan-genomic analysis also showed that 67 genes could only be found in humoral isolates. ReX3 and DUF853 domain protein were found in all eight human isolates. The composition of unique genes in humoral isolate genomes indicated that the transcriptional regulators may be important when Nocardia invades the host, which allows them to survive in the new ecological system. Conclusion In this study, we confirmed that NT001 could cause infected animal death, and identified many possible virulence factors for our future studies. This study also provides new insight for our further study on Nocardia virulence mechanisms.

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The First Report of Cerebral Nocardiosis Caused by Nocardia terpenica Together With Exiguobacterium profundum Bacteremia

Cited by 7 publications

References 16 publications

Challenges in microbiological identification of aerobic bacteria isolated from the skin of reptiles

Challenges in microbiological identification of aerobic bacteria isolated from the skin of reptiles

Brain Abscess Due To Asian Nocardia in a 63 Years-Old Patient with Adult Still´S Disease: A Rare Case Report and Literature Review

A Complete Genome of Nocardia terpenica NC_YFY_NT001 and Pan-Genomic Analysis Based on Different Sources of Nocardia spp. Isolates Reveal Possibly Host-Related Virulence Factors

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