The first SERRS multiplexing from labelled oligonucleotides in a microfluidics lab-on-a-chip

Docherty, F. T.; Monaghan, Paul; Keir, Ruth; Graham, Duncan; Smith, W. Ewen; Cooper, Jonathan M.

doi:10.1039/b311589e

Cited by 67 publications

(69 citation statements)

References 6 publications

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“…Docherty et al [94] reported the SERRS detection of three different dye-labeled DNA oligonucleotides with Escherichia coli sequences in a PDMS microfluidic chip. In this work, the SERRS signals for the mixtures of DNA oligonucleotides and aggregated silver colloids in the channel were measured under static conditions.…”

Section: Biological Analysis and Biomedical Diagnosticsmentioning

confidence: 99%

Recent advances in surface‐enhanced Raman scattering detection technology for microfluidic chips

2008

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Microfluidic chip devices and their application to sensitive chemical and biological analyses have attracted significant attention over the past decade. The miniaturization of reaction systems offers practical advantages over conventional benchtop systems. In this case, however, a highly sensitive on-chip detection method is important for the monitoring of chemical reactions as well as for the detection of analytes inside the channel because the detection volume in a micrometer-size channel is extremely small. Recently, a surface-enhanced Raman scattering (SERS) technique is being regarded as a potential candidate for the highly sensitive detection of analytes in a microfluidic chip. This review provides a general survey and an in-depth look at recent developments in SERS techniques for the biological/environmental analysis of minute analytes in a microfluidic chip.

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Section: Biological Analysis and Biomedical Diagnosticsmentioning

confidence: 99%

Recent advances in surface‐enhanced Raman scattering detection technology for microfluidic chips

2008

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“…However, SERRS only occurs with analytes that contain a chromophore with an absorption that matches the wavelength of the excitation source. It has been demonstrated that SERRS can be more sensitive than fluorescence measurements made on standard commercial instrumentation [25], can be successfully applied to microfluidic devices [26], and with the proper Page 4 of 9 chromophores deliver a quantitative analytical method down to 10 -11 M concentrations [27]. Oligosaccharides are another important class of complex biological molecules where SERS has been used for both the identification of closely related species, and the quantitative analysis of oligosaccharides mixtures [28].…”

Section: Sers Of Small Biological Moleculesmentioning

confidence: 99%

Surface enhanced Raman scattering for narcotic detection and applications to chemical biology

Ryder

2005

Current Opinion in Chemical Biology

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Raman spectroscopy is rapidly finding favour for applications in the life science due to the ease with which it can be used to extract significant data from tissue and cells. However, the Raman effect is an inherently weak effect, which hinders the analysis of low concentration analytes. The Raman sensitivity can be improved via the Surface Enhanced Raman Scattering (SERS) effect. In SERS, Raman spectra are dramatically increased when a molecule is adsorbed onto nanoroughened noble metal surfaces such as silver and gold. The degree of enhancement enables single molecule detection, which offers the potential for the unambiguous identification of analytes at concentrations that are useful in both a forensic and a chemical biology context. Here we discuss some of the practical applications of SERS to both low-level narcotic detection, and how this can be applied to chemical biology.

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“…Approaches to minimise these disadvantages have been reported in the literature. [15] When using inexpensive and disposable PDMS chips the problem can be circumvented, renewing the chip after each measurement. More expensive glass chips can be regenerated by washing steps with aggressive chemicals such as aqua regina or piranha solution after each measurement.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Online Detection of Low‐Concentrated Drugs via a SERS Microfluidic System

2007

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Herein, quantitative online monitoring of concentration fluctuations of different interesting drugs, namely, the phenothiazine promethazine as well as the anti-cancer agent mitoxantrone via surface enhanced Raman scattering assay based on a microfluidic device is demonstrated. With the applied liquid/liquid two-phase-segmented flow system we succeed in preventing the adhesion of nanoparticle aggregates to the channel walls which is necessary for a quantitative analysis. Even after repeated cycles no carry-over due to sedimentation of colloid particles is observed. To the best of our knowledge these are the first measurements applying a combination of a microfluidic device with SERS detection for quantitative online monitoring of fluctuations in drug concentrations over hours without use of aggressive chemicals for rinsing the chip surfaces prior to each measurement.

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The first SERRS multiplexing from labelled oligonucleotides in a microfluidics lab-on-a-chip

Abstract: The first simultaneous detection of three dye-labelled oligonucleotides in a microfluidics chip by SERRS is reported.

Cited by 67 publications

References 6 publications

Recent advances in surface‐enhanced Raman scattering detection technology for microfluidic chips

Recent advances in surface‐enhanced Raman scattering detection technology for microfluidic chips

Surface enhanced Raman scattering for narcotic detection and applications to chemical biology

Quantitative Online Detection of Low‐Concentrated Drugs via a SERS Microfluidic System

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