The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

“…The double mutants between mis3-224 (Takahashi et al e1994) and various mutations noted below were constructed; cdc2-33, cdc6-121, cdc13-117, cdc25-22, cdc10-129 (Nurse et al 1976), cdc2-3w (Enoch et al 1992), Dcyc17/cig2 (Obara-Ishihara & Okayama 1994), Dmik1 (Lundgren et al 1991), wee1-50 (Russell & Nurse 1987), cdc16-116 (Fankhauser et al 1993), cdc17-K42, cdc18-K46 (Nasmyth & Nurse 1981), cdc22-C1 (Fernandez Sarabia et al 1993), swi7-H4 (Singh & Klar 1993), mis5-268, mis11-453 (Takahashi et al 1994, Drad3, Drad9, Drad17, Drad24, Drad25, Dchk1 (Al-Khodairy et al 1994), cut5-T401 (Saka et al 1994), Dcds1 (Murakami & Okayama 1995), Ddis1 (Nabeshima et al 1995), dis3-54 (Kinoshita et al 1991), dis2-11, Ddis2 (Ohkura et al 1989), cut1-21, cut2-364 (Funabiki et al 1996, cut9 (Yamada et al 1997), Dppa1, Dppa2 (Kinoshita et al 1993),Dpck1 (Toda et al 1993), Dtop1, top2-191 (Uemura & Yanagida 1984), and Ddsk1 mutants (Takeuchi & Yanagida 1993). The Mis3-HA integrant was obtained by integrating the HA-tagged mis3 gene at the carboxyl-terminus to replace the genomic mis3 locus.…”

Assignment of the gene encoding type 1γ protein phosphatase catalytic subunit (PPP1CC) on human, rat, and mouse chromosomes

“…They also explained that the rat Ppplcc is homologous to a gene for phosphoserine phosphatase (PSP) which has been mapped on HSA7 and MMU5 (Lalley and Diaz, 1984;Danesino et al, 1987). However, it should be mentioned that PPly belongs to the protein serine/threonine phosphatase type 1 family that removes phosphate from serine and/or threonine of phosphoproteins (Ohkura et aL, 1989;Sasaki et al, 1990;Barker et aL, 1993), whereas PSP fills an important role in the biosynthesis of serine from carbohydrates by catalyzing the last step, hydrolysis of O-phosphoserine (MoroFurlani et al, 1980). Therefore, these two enzymes belong to a quite different family that is encoded by each different gene.…”

“…These findings demonstrate that the PP1 is a very highly conservative gene family with at least three different genes. PPI~, is one of them that have been cloned in human (Barker et aL, 1993), rat (Sasaki et al, 1990), and mouse (Ohkura et al, 1989; mouse PPly2 is designated as dis2ml) which showed a high degree of homology with each other. Comparative study of nucleotide and deduced amino acid sequences of PP13,1 and PP13,2 suggest that they are derived from the same gene and produced by alternative splicing (Sasaki et al, 1990;Barker et al, 1993).…”

Sequence homology between purple acid phosphatases and phosphoprotein phosphatases

“…Conserved residues in all or all but one sequence are notated with a bar at the top and bottom of the column and capitalized; conservative substitutions are capitalized. For convenience, the list of PP sequences is not inclusive; however, the conserved metal binding ligands are unaffected by incorporation of the additional sequences [5,10,11,[17][18][19]. therefore, reflect the presence of a common or at least related metal-containing active site in both proteins.…”

“…Very recently, the nucleotide sequence encoding some proteins from cerCorrespondence address: B.A. Averill, Department of Chemistry, University of Virginia, Charlottesville, VA 22901, USA tain fungi and Drosophila have been shown to be highly homologous (50-90°70) to those of mammalian PP1 and PP2A [13,[16][17][18][19][20], suggesting that the phosphoprotein phosphatases may be widely distributed.…”