The Fission Yeast Mating-Type Switching Motto: “One-for-Two” and “Two-for-One”

Arcangioli, Benoı̂t; Gangloff, Serge

doi:10.1128/mmbr.00008-21

Cited by 9 publications

(7 citation statements)

References 217 publications

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“…The donor choice mechanism in MTS is highly regulated by the MTS factor Swi2-Swi5, chromatin formation, and cis -acting SRE2 and SRE3 elements ( 17 , 48 ). The Swi2-Swi5 complex localizes to SRE3 independent of Swi6 and to SRE2 dependent on Swi6, and has been hypothesized to promote the strand exchange reaction for MTS ( 3 , 4 ).…”

Section: Discussionmentioning

confidence: 99%

Fission yeast Swi2 designates cell-type specific donor and stimulates Rad51-driven strand exchange for mating-type switching

Maki

Thon

Iwasaki

2023

Nucleic Acids Research

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A haploid of the fission yeast Schizosaccharomyces pombe expresses either the P or M mating-type, determined by the active, euchromatic, mat1 cassette. Mating-type is switched by Rad51-driven gene conversion of mat1 using a heterochromatic donor cassette, mat2-P or mat3-M. The Swi2-Swi5 complex, a mating-type switching factor, is central to this process by designating a preferred donor in a cell-type-specific manner. Swi2-Swi5 selectively enables one of two cis-acting recombination enhancers, SRE2 adjacent to mat2-P or SRE3 adjacent to mat3-M. Here, we identified two functionally important motifs in Swi2, a Swi6 (HP1 homolog)-binding site and two DNA-binding AT-hooks. Genetic analysis demonstrated that the AT-hooks were required for Swi2 localization at SRE3 to select the mat3-M donor in P cells, while the Swi6-binding site was required for Swi2 localization at SRE2 to select mat2-P in M cells. In addition, the Swi2-Swi5 complex promoted Rad51-driven strand exchange in vitro. Taken together, our results show how the Swi2-Swi5 complex would localize to recombination enhancers through a cell-type specific binding mechanism and stimulate Rad51-driven gene conversion at the localization site.

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Section: Discussionmentioning

confidence: 99%

Fission yeast Swi2 designates cell-type specific donor and stimulates Rad51-driven strand exchange for mating-type switching

Maki

Thon

Iwasaki

2023

Nucleic Acids Research

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“…Adaptability to starvation is important for many microbes because the period of nutrient starvation is longer than that of nutrient abundance in nature (Fujita et al, 2007). Starvation‐induced stress causes S. pombe to enter the stationary phase, which arrests the cell cycle at G 0 , or induces a sexual differentiation response (Arcangioli & Gangloff, 2023; Tsuyuzaki et al, 2021). Under conditions in which sexual differentiation is not possible, starvation, such as insufficient nitrogen or sulfur, leads to cell cycle arrest at G 0 (through G 1 and G 2 , respectively) and an approximately 1‐month extension of CLS (Ohtsuka et al, 2017; Ohtsuka, Hatta, et al, 2022; Su et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

ecl family genes: Factors linking starvation and lifespan extension in Schizosaccharomyces pombe

Ohtsuka

Otsubo

Shimasaki

et al. 2023

Molecular Microbiology

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In the fission yeast Schizosaccharomyces pombe, the duration of survival in the stationary phase, termed the chronological lifespan (CLS), is affected by various environmental factors and the corresponding gene activities. The ecl family genes were identified in the genomic region encoding non‐coding RNA as positive regulators of CLS in S. pombe, and subsequently shown to encode relatively short proteins. Several studies revealed that ecl family genes respond to various nutritional starvation conditions via different mechanisms, and they are additionally involved in stress resistance, autophagy, sexual differentiation, and cell cycle control. Recent studies reported that Ecl family proteins strongly suppress target of rapamycin complex 1, which is a conserved eukaryotic nutrient‐sensing kinase complex that also regulates longevity in a variety of organisms. In this review, we introduce the regulatory mechanisms of Ecl family proteins and discuss their emerging findings.

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“…As a result, in a population of homothallic cells, both mating types are present, allowing for sexual reproduction to occur in adverse nutritional conditions. The molecular mechanisms underlying MTS have been extensively studied and rely on a complex process involving an imprint, referred to as the switching of mating type (smt) signal, and recombination of the donor material of the mat2-P or mat3-M locus at mat1 , two events that are associated with DNA replication (Arcangioli & Klar, 1991; Arcangioli & Gangloff, 2023).…”

Section: Introductionmentioning

confidence: 99%

Engineering heterothallic strains in fission yeast

García-Ruano,

Hsu,

Leray

et al. 2023

Preprint

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In poor nitrogen conditions, fission yeast cells mate, undergo meiosis and form spores that are resistant to deleterious environments. Natural isolates of Schizosaccharomyces pombe are homothallic. This allows them to naturally switch between the two h- and h+ mating types with a high frequency, thereby ensuring the presence of both mating partners in a population of cells. However, alteration of the mating type locus can abolish mating type switching or reduce it to a very low frequency. Such heterothallic strains have been isolated and are common in research laboratories due to the simplicity of their use for Mendelian genetics. In addition to the standard laboratory strains, a large collection of natural S. pombe isolates is now available, representing a powerful resource for investigating the genetic diversity and biology of fission yeast. However, most of these strains are homothallic, and only tedious or mutagenic strategies have been described to obtain heterothallic cells from a homothallic parent. Here, we describe a simple approach to generate heterothallic strains. It takes advantage of an alteration of the mating type locus that was previously identified in a mating type switching-deficient strain and the CRISPR-Cas9 editing tool, allowing for a one-step engineering of heterothallic cells with high efficiency.

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The Fission Yeast Mating-Type Switching Motto: “One-for-Two” and “Two-for-One”

Cited by 9 publications

References 217 publications

Fission yeast Swi2 designates cell-type specific donor and stimulates Rad51-driven strand exchange for mating-type switching

Fission yeast Swi2 designates cell-type specific donor and stimulates Rad51-driven strand exchange for mating-type switching

ecl family genes: Factors linking starvation and lifespan extension in Schizosaccharomyces pombe

Engineering heterothallic strains in fission yeast

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