The Flagella of an Atypical Enteropathogenic
            <i>Escherichia coli</i>
            Strain Are Required for Efficient Interaction with and Stimulation of Interleukin-8 Production by Enterocytes In Vitro

Sampaio, Suely Carlos Ferreira; Gomes, Tânia A. T.; Pichon, Christophe; Merle, Laurence du; Guadagnini, Stéphanie; Abe, Cecília M.; Sampaio, Julliane Messias Cordeiro; Bouguénec, Chantal Le

doi:10.1128/iai.00177-09

Cited by 32 publications

(53 citation statements)

References 49 publications

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“…The flagellum of aEPEC plays a role in adhesion and in the aEPEC-induced early, but not late, IL-8 production in fully differentiated Caco-2 cells (488,782). aEPEC adhering to fully differentiated, mucin-secreting HT29-MTX cells induces mucus hypersecretion, whereas tEPEC does not (783).…”

Section: Host Cellular Defense Responsesmentioning

confidence: 99%

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Moal

Servin

2013

Microbiol Mol Biol Rev

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SUMMARY Hosts are protected from attack by potentially harmful enteric microorganisms, viruses, and parasites by the polarized fully differentiated epithelial cells that make up the epithelium, providing a physical and functional barrier. Enterovirulent bacteria interact with the epithelial polarized cells lining the intestinal barrier, and some invade the cells. A better understanding of the cross talk between enterovirulent bacteria and the polarized intestinal cells has resulted in the identification of essential enterovirulent bacterial structures and virulence gene products playing pivotal roles in pathogenesis. Cultured animal cell lines and cultured human nonintestinal, undifferentiated epithelial cells have been extensively used for understanding the mechanisms by which some human enterovirulent bacteria induce intestinal disorders. Human colon carcinoma cell lines which are able to express in culture the functional and structural characteristics of mature enterocytes and goblet cells have been established, mimicking structurally and functionally an intestinal epithelial barrier. Moreover, Caco-2-derived M-like cells have been established, mimicking the bacterial capture property of M cells of Peyer's patches. This review intends to analyze the cellular and molecular mechanisms of pathogenesis of human enterovirulent bacteria observed in infected cultured human colon carcinoma enterocyte-like HT-29 subpopulations, enterocyte-like Caco-2 and clone cells, the colonic T84 cell line, HT-29 mucus-secreting cell subpopulations, and Caco-2-derived M-like cells, including cell association, cell entry, intracellular lifestyle, structural lesions at the brush border, functional lesions in enterocytes and goblet cells, functional and structural lesions at the junctional domain, and host cellular defense responses.

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Section: Host Cellular Defense Responsesmentioning

confidence: 99%

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Moal

Servin

2013

Microbiol Mol Biol Rev

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“…Zeo-resistant clones that were unable to grow in Apra-containing medium were selected and stored at Ϫ80°C. Homologous recombination events were confirmed by PCR with primers flanking the fliD gene (fliD.verifF-5=/fliD.verifR-3=) ( Table 2) (22).…”

Section: Methodsmentioning

confidence: 99%

“…The mutants and wild-type strains were assayed for motility as previously described by Sampaio et al (22). Motility was demonstrated by bacterial spreading around the initial inoculum, as evidenced by increased turbidity.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

“…In addition, we have recently shown that flagellum expression by aEPEC strain 1711-4 (serotype O51:H40) is involved in binding to human enterocytes in vitro (22). We hypothesized that the flagellar cap protein (FliD) could mediate the binding of bacteria to microvillus tips, since electron micrographs of Caco-2 cells infected with strain 1711-4 show numerous bacteria adhering to cells by their flagellar tips (22). In the present study, we demonstrated that FliD mediates the adhesion of aEPEC, as well as of other prototype diarrheagenic E. coli strains, to human enterocytes under in vitro conditions and is therefore a relevant virulence-associated factor expressed by these bacteria.…”

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confidence: 99%

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Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli

et al. 2016

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The expression of flagella correlates with different aspects of bacterial pathogenicity, ranging from adherence to host cells to activation of inflammatory responses by the innate immune system. In the present study, we investigated the role of flagella in the adherence of an atypical enteropathogenic Escherichia coli (aEPEC) strain (serotype O51:H40) to human enterocytes. Accordingly, isogenic mutants deficient in flagellin (FliC), the flagellar structural subunit; the flagellar cap protein (FliD); or the MotAB proteins, involved in the control of flagellar motion, were generated and tested for binding to differentiated Caco-2 cells. Binding of the aEPEC strain to enterocytes was significantly impaired in strains with the fliC and fliD genes deleted, both of which could not form flagella on the bacterial surface. A nonmotile but flagellated MotAB mutant also showed impaired adhesion to Caco-2 cells. In accordance with these observations, adhesion of aEPEC strain 1711-4 to Caco-2 cells was drastically reduced after the treatment of Caco-2 cells with purified FliD. In addition, incubation of aEPEC bacteria with specific anti-FliD serum impaired binding to Caco-2 cells. Finally, incubation of Caco-2 cells with purified FliD, followed by immunolabeling, showed that the protein was specifically bound to the microvillus tips of differentiated Caco-2 cells. The aEPEC FliD or anti-FliD serum also reduced the adherence of prototype typical enteropathogenic, enterohemorrhagic, and enterotoxigenic E. coli strains to Caco-2 cells. In conclusion, our findings further strengthened the role of flagella in the adherence of aEPEC to human enterocytes and disclosed the relevant structural and functional involvement of FliD in the adhesion process.

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Intestinal Goblet Cell-Expressed Reg4 Ameliorates Intestinal Inflammation Potentially by Restraining Pathogenic Escherichia coli Infection

Lu,

Wu,

Wang

et al. 2024

Probiotics & Antimicro. Prot.

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The Flagella of an Atypical Enteropathogenic Escherichia coli Strain Are Required for Efficient Interaction with and Stimulation of Interleukin-8 Production by Enterocytes In Vitro

Cited by 32 publications

References 49 publications

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli

Intestinal Goblet Cell-Expressed Reg4 Ameliorates Intestinal Inflammation Potentially by Restraining Pathogenic Escherichia coli Infection

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