2020
DOI: 10.7554/elife.61446
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The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching

Abstract: The bacterial flagellar motor switches rotational directions between counterclockwise (CCW) and clockwise (CW) to direct the migration of the cell. The cytoplasmic ring (C-ring) of the motor, which is composed of FliG, FliM, and FliN, is known for controlling the rotational sense of the flagellum. However, the mechanism underlying rotational switching remains elusive. Here, we deployed cryo-electron tomography to visualize the C-ring in two rotational biased mutants in Vibrio alginolyticus. We determined the C… Show more

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Cited by 56 publications
(95 citation statements)
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References 92 publications
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“…The model is consistent with the recently observed structural changes in the C-ring of CCW-biased and CW-locked mutants of V. alginolyticus (59). Furthermore, it predicts that the reversal of the ion motive force would invert the rotation direction of the stator unit and hence of the motor.…”
Section: Powering Bidirectional Rotation Of the Flagellumsupporting
confidence: 87%
“…The model is consistent with the recently observed structural changes in the C-ring of CCW-biased and CW-locked mutants of V. alginolyticus (59). Furthermore, it predicts that the reversal of the ion motive force would invert the rotation direction of the stator unit and hence of the motor.…”
Section: Powering Bidirectional Rotation Of the Flagellumsupporting
confidence: 87%
“…During CCW rotation, the C-ring adopts a narrow conformation and stator units interact with the outside of the ring (32, 42). Upon binding the chemotaxis response regulator CheY-P, the C-ring expands and the stator units now interact with the inside of the C-ring, driving CW rotation (32, 42). These structural changes in the C-ring are the likely source of the asymmetry in the torque-speed relationship of CCW and CW rotating flagellar motors (31, 33).…”
Section: Discussionmentioning
confidence: 99%
“… Classification of flagellar motors based on their scaffolds. The figures were prepared using electron cryotomography maps for the flagellar motors from: (A) Escherichia coli (EMDB-5311; Chen et al, 2011 ); (B) Salmonella enterica (EMDB-5310; Chen et al, 2011 ); (C) Vibrio fischeri (EMDB 3155; Beeby et al, 2016 ); (D) Vibrio alginolyticus (EMDB-21027 and EMDB-21819; Carroll et al, 2020 ; Zhu et al, 2020 ); (E) Treponema primitia (EMDB-1235; Murphy et al, 2006 ); (F) Borrelia burgdorferi (EMDB-9122; Qin et al, 2018 ); (G) Helicobacter pylori (EMDB-8459; Qin et al, 2017 ); and (H) Campylobacter jejuni (EMDB-3150; Beeby et al, 2016 ). …”
Section: First Electron Cryotomography Motor Reconstructions: the Dismentioning
confidence: 99%