The flavonoid biosynthetic pathway in plants: Function and evolution

Koes, Ronald; Quattrocchio, Francesca; Mol, J.A.

doi:10.1002/bies.950160209

Cited by 655 publications

(417 citation statements)

References 83 publications

Supporting

Mentioning

402

Contrasting

Unclassified

Order By: Relevance

“…It is interesting that while several other proteins in this pathway were down-regulated in 'Frida' in response to cold stress, CHS, the first committed protein in the flavonoid pathway (Winkel-Shirley, 2001), as well as F3H are strongly up-regulated in response to cold stress. It is important to note that since both CHS and F3H have been characterized as rate-limiting enzymes (Koes et al, 1994), the data suggest a strongly enhanced ability for 'Frida' to synthesize flavonoid products. In contrast, 'Jonsok' showed a significant cold-related decrease in CHS and F3H.…”

Section: Proteins Involved In the Phenylpropanoid Biosynthetic Pathwaymentioning

confidence: 94%

See 1 more Smart Citation

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Koehler

Wilson²,

Goodpaster

et al. 2012

Plant Physiology

View full text Add to dashboard Cite

To gain insight into the molecular basis contributing to overwintering hardiness, a comprehensive proteomic analysis comparing crowns of octoploid strawberry (Fragaria 3 ananassa) cultivars that differ in freezing tolerance was conducted. Four cultivars were examined for freeze tolerance and the most cold-tolerant cultivar ('Jonsok') and least-tolerant cultivar ('Frida') were compared with a goal to reveal how freezing tolerance is achieved in this distinctive overwintering structure and to identify potential cold-tolerance-associated biomarkers. Supported by univariate and multivariate analysis, a total of 63 spots from twodimensional electrophoresis analysis and 135 proteins from label-free quantitative proteomics were identified as significantly differentially expressed in crown tissue from the two strawberry cultivars exposed to 0-, 2-, and 42-d cold treatment. Proteins identified as cold-tolerance-associated included molecular chaperones, antioxidants/detoxifying enzymes, metabolic enzymes, pathogenesis-related proteins, and flavonoid pathway proteins. A number of proteins were newly identified as associated with cold tolerance. Distinctive mechanisms for cold tolerance were characterized for two cultivars. In particular, the 'Frida' cold response emphasized proteins specific to flavonoid biosynthesis, while the more freezing-tolerant 'Jonsok' had a more comprehensive suite of known stress-responsive proteins including those involved in antioxidation, detoxification, and disease resistance. The molecular basis for 'Jonsok'-enhanced cold tolerance can be explained by the constitutive level of a number of proteins that provide a physiological stress-tolerant poise. Strawberry (Fragaria 3 ananassa) cultivation predominates in regions with mild winters. In colder climates, overwintering hardiness is an essential trait for strawberry cultivation. Freezing injury of strawberry plants is one of the greatest factors in reducing crop yield and quality in temperate regions. Winter damage in Norway, for example, on average causes losses of 20%. Thus, production of cultivars with improved freezing hardiness is one of Norway's major objectives for their strawberry breeding programs. Improvement of cold hardiness is desirable for securing economic sustainability of the existing crops, and for expanding the growing regions of temperate fruit crops. Because strawberry is a representative species for the Rosaceae crops (e

show abstract

Section: Proteins Involved In the Phenylpropanoid Biosynthetic Pathwaymentioning

confidence: 94%

“…These compounds function in pigments, regulation of plant growth, antimicrobials, cell wall modifications, and antioxidants (Koes et al, 1994;Winkel-Shirley, 2001;Dixon and Pasinetti, 2010;Vogt, 2010).…”

Section: Proteins Involved In the Phenylpropanoid Biosynthetic Pathwaymentioning

confidence: 99%

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Koehler

Wilson²,

Goodpaster

et al. 2012

Plant Physiology

View full text Add to dashboard Cite

show abstract

“…Trans-cinnamic acid is also a precursor of flavonols (Koes et al, 1994) that accumulate in the white Mitchell petals (Van der Meer et al, 1992), where flavonol-synthesizing enzymes are expressed. Because of a mutation in the regulatory AN2 gene in Mitchell (Quattrocchio et al, 1999), anthocyanin production is reduced in petal limbs but not in the tubes.…”

Section: Odo1 Suppression Does Not Affect Colormentioning

confidence: 99%

ODORANT1Regulates Fragrance Biosynthesis in Petunia Flowers

et al. 2005

View full text Add to dashboard Cite

Floral scent is important to plant reproduction because it attracts pollinators to the sexual organs. Therefore, volatile emission is usually tuned to the foraging activity of the pollinators. In Petunia hybrida, volatile benzenoids determine the floral aroma. Although the pathways for benzenoid biosynthesis have been characterized, the enzymes involved are less well understood. How production and emission are regulated is unknown. By targeted transcriptome analyses, we identified ODORANT1 (ODO1), a member of the R2R3-type MYB family, as a candidate for the regulation of volatile benzenoids in Petunia hybrida cv W115 (Mitchell) flowers. These flowers are only fragrant in the evening and at night. Transcript levels of ODO1 increased before the onset of volatile emission and decreased when volatile emission declined. Downregulation of ODO1 in transgenic P. hybrida Mitchell plants strongly reduced volatile benzenoid levels through decreased synthesis of precursors from the shikimate pathway. The transcript levels of several genes in this pathway were reduced by suppression of ODO1 expression. Moreover, ODO1 could activate the promoter of the 5-enol-pyruvylshikimate-3-phosphate synthase gene. Flower pigmentation, which is furnished from the same shikimate precursors, was not influenced because color and scent biosynthesis occur at different developmental stages. Our studies identify ODO1 as a key regulator of floral scent biosynthesis.

show abstract

“…It has long been proposed that flavonols act as interna1 UV-screening molecules for protecting photosynthetic tissues (Koes et al, 1994;Shirley, 1996). Analyses of mutants defective in flavonoid biosynthesis have indicated the importance of flavonoids for UV tolerance (Lois and Buchanan, 1994;Shirley, 1996).…”

Section: A Role Of Flavonoids In U V Tolerancementioning

confidence: 99%