The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study

Téglás, Tímea; Cziáky, Zoltán; Oláh, Neli-Kinga; Nyakas, Csaba; Máthé, Endre

doi:10.3390/molecules28083571

Cited by 7 publications

(6 citation statements)

References 66 publications

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“…The last three compounds were classified as other phenolic compounds: p-coumaric acid glucose ester (4) and two compounds from the catechin group (1 and 5) (Table 2). Most of the identified compounds have previously been detected in blackcurrant leaf extracts [35][36][37][38]. In the phenolic compound profile obtained in the present work, 17 compounds were identical to those reported by D'Urso et al [36], who identified 31 phenolic compounds in 3 types of blackcurrant leaf extracts.…”

Section: Chromatographic Analysessupporting

confidence: 88%

Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts

Staszowska-Karkut,

Chilczuk,

Materska

et al. 2023

Molecules

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The aim of this study was to determine the relationship between antioxidant and anticancer properties of extracts from blackcurrant (Ribes nigrum L.) leaves and their fractions and chemical contents. Dried ethanolic extract was divided into three fractions using solid phase extraction: aqueous (F1), 40% MeOH (F2), and 70% MeOH (F3). Both the extract and the fractions were analyzed in terms of antiradical activity (DPPH• and ABTS+•), total phenolic compounds, and total flavonoids. The antitumor potential of the fractions was evaluated in vitro on human colorectal (HCT 116) and prostate (PC-3) cancer cells. Phenolics were identified using HPLC-QTOF-MS, and twelve compounds were quantified by HPLC-DAD. Finally, principal component analysis was carried out to assess the relationship between the tested factors. The results confirmed that blackcurrant leaves are a rich source of phenolics with high antioxidant activity and anticancer properties. It was demonstrated that the F2 fraction had the highest content of phenolics and the highest antiradical activity. Additionally, only this fraction showed cytotoxic activity against HCT 116 cells. It was confirmed that both the blackcurrant leaf extract and its fractions are a promising source of condensed active compounds and can be used as natural functional food additives.

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Section: Chromatographic Analysessupporting

confidence: 88%

Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts

Staszowska-Karkut,

Chilczuk,

Materska

et al. 2023

Molecules

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“…However, there is a paucity of data in the literature on the antimicrobial activity of plant parts, such as buds and young shoots, despite the fact that they contain many bioactive compounds and could have potent antimicrobial properties. In addition, recent studies have described the phytonutrient profile of some GTEs while also revealing their nutritional, antidiabetic and anti-inflammatory effects [40,48,51]. The phytonutrient composition of these GTEs also suggests that they may have antimicrobial properties, and this article presents relevant research data.…”

Section: Discussionmentioning

confidence: 94%

“…Similarly, the antioxidant capacity of blackcurrant was found to vary depending on the season and cultivar [46]. In blackcurrant buds, the most common phenolic compounds are gallic acid, hydroxycinnamic acid derivatives, several flavonols (glycosidealcoolics of quercetin, myricetin, kaempferol and isorhamnetin) and dihydroquercetin derivatives [20,48]. According to another study, the most common flavonols in blackcurrant buds are rutin, isoquercetin and astragalin [46,65].…”

Section: The Blackcurrant Gte Is Less Effective As a Microbial Growth...mentioning

confidence: 97%

“…Kendir et al (2019) [47] reported that blackcurrant leaf extracts had wound-healing effects, the activity of which could be attributed to phenolic compounds, especially chlorogenic acid and rutin. Another work of research showed that blackcurrant GTEs prevented neuroinflammation in rats that underwent lipopolysaccharide treatment [48].…”

Section: Blackcurrant (Ribes Nigrum L)mentioning

confidence: 99%

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Specific Antimicrobial Activities Revealed by Comparative Evaluation of Selected Gemmotherapy Extracts

Héjja,

Mihok,

Alaya

et al. 2024

Antibiotics

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Nowadays, unprecedented health challenges are urging novel solutions to address antimicrobial resistance as multidrug-resistant strains of bacteria, yeasts and moulds are emerging. Such microorganisms can cause food and feed spoilage, food poisoning and even more severe diseases, resulting in human death. In order to overcome this phenomenon, it is essential to identify novel antimicrobials that are naturally occurring, biologically effective and increasingly safe for human use. The development of gemmotherapy extracts (GTEs) using plant parts such as buds and young shoots has emerged as a novel approach to treat/prevent human conditions due to their associated antidiabetic, anti-inflammatory and/or antimicrobial properties that all require careful evaluations. Seven GTEs obtained from plant species like the olive (Olea europaea L.), almond (Prunus amygdalus L.), black mulberry (Morus nigra L.), walnut (Juglans regia L.), blackberry (Rubus fruticosus L.), blackcurrant (Ribes nigrum L.) and bilberry (Vaccinium myrtillus L.) were tested for their antimicrobial efficiency via agar diffusion and microbroth dilution methods. The antimicrobial activity was assessed for eight bacterial (Bacillus cereus, Staphylococcus aureus, Salmonella enterica subsp. enterica, Proteus vulgaris, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Listeria monocytogenes), five moulds (Aspergillus flavus, Aspergillus niger, Aspergillus ochraceus, Penicillium citrinum, Penicillium expansum) and one yeast strain (Saccharomyces cerevisiae). The agar diffusion method revealed the blackberry GTE as the most effective since it inhibited the growth of three bacterial, four moulds and one yeast species, having considered the total number of affected microorganism species. Next to the blackberry, the olive GTE appeared to be the second most efficient, suppressing five bacterial strains but no moulds or yeasts. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were then determined for each GTE and the microorganisms tested. Noticeably, the olive GTE appeared to feature the strongest bacteriostatic and bactericidal outcome, displaying specificity for S. aureus, E. faecalis and L. monocytogenes. The other GTEs, such as blueberry, walnut, black mulberry and almond (the list indicates relative strength), were more effective at suppressing microbial growth than inducing microbial death. However, some species specificities were also evident, while the blackcurrant GTE had no significant antimicrobial activity. Having seen the antimicrobial properties of the analysed GTEs, especially the olive and black mulberry GTEs, these could be envisioned as potential antimicrobials that might enhance antibiotic therapies efficiency, while the blackberry GTE would act as an antifungal agent. Some of the GTE mixtures analysed have shown interesting antimicrobial synergies, and all the antimicrobial effects observed argue for extending these studies to include pathological microorganisms.

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“…Several components found in R. nigrum leaves, such as quercetin and anthocyanins, have anti-in ammatory, antioxidant, antiviral, antimicrobial, antibiotic-modulatory and other effects [22]. Some authors mention the neuroprotective effect of R. nigrum extracts [59].…”

Section: Discussionmentioning

confidence: 99%

Ribes nigrum leaf extract: antioxidant capacity and redox balance regulation mechanism in microglial cells

Minasyan,

Pires,

Gondcaille

et al. 2024

Preprint

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This study focuses on the investigation of the antioxidant and anti-inflammatory activity of extracts from Ribes nigrum leaves on BV-2 microglial Wt and Acyl-CoA oxidase 1 deficient (Acox1−/−) cell line models, useful for the investigation of some neurodegenerative disorders. Investigations showed the presence of high quantity of phenolic compounds in this extract, among which flavan-3-ols, flavonols, furanocoumarins, hydroxycinnamates, quercetin, and quercetin derivatives were major components, which are known for their biological activity in various test systems. The MTT test revealed the 0.125 mg/mL concentration of R. nigrum extract as the highest non-toxic. The investigated extract showed high antioxidant activity in chemical-based tests. The antioxidant potential of the R. nigrum leaf extract was furtherly explored using the BV-2 microglial cell line models. Moreover, the extract was found to alter the activity of the main antioxidant enzyme, catalase and fatty acid oxidation enzyme, Acyl-CoA oxidase 1 (ACOX1) as well as the expression of appropriate genes in Wt and Acox1−/− BV-2 microglial cells such as Cat, iNos, Il-1β, Tnf-α, and Abcd1. In Wt cells, the treatment with R. nigrum leaf extract ACOX1 activity was downregulated after 24 hours of treatment, meanwhile the catalase activity remains unchanged. The further treatment leaded to the downregulation of catalase and the upregulation of ACOX1 activity. However, in Acox1−/− cells, which represent a model of oxidative stress, an increase in catalase activity was observed only after 48 hours of treatment, indicating an alternative mode of action of the extract. It was also observed the reduced ROS and NO formation in cells, indicating the pronounced antioxidant capacity of R. nigrum extract in the investigated cell-models.

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The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study

Cited by 7 publications

References 66 publications

Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts

Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts

Specific Antimicrobial Activities Revealed by Comparative Evaluation of Selected Gemmotherapy Extracts

Ribes nigrum leaf extract: antioxidant capacity and redox balance regulation mechanism in microglial cells

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