The Flavouring Phytochemical 2-Pentanone Reduces Prostaglandin Production and COX-2 Expression in Colon Cancer Cells

Pettersson, Jenny; Karlsson, Pernilla Christina; Göransson, Ulf; Rafter, Joseph; Bohlin, Lars

doi:10.1248/bpb.31.534

Cited by 28 publications

(22 citation statements)

References 46 publications

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“…According to Pettersson et al . [48], it inhibits the prostaglandin production and COX-2 protein expression in human colon cancer cells. The increase of 2,3-butanedione is interesting since it may have health benefits by impacting on the growth of some bacteria, such as L. delbrueckii subsp.…”

Section: Discussionmentioning

confidence: 99%

Impact of a synbiotic food on the gut microbial ecology and metabolic profiles

et al. 2010

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BackgroundThe human gut harbors a diverse community of microorganisms which serve numerous important functions for the host wellbeing. Functional foods are commonly used to modulate the composition of the gut microbiota contributing to the maintenance of the host health or prevention of disease. In the present study, we characterized the impact of one month intake of a synbiotic food, containing fructooligosaccharides and the probiotic strains Lactobacillus helveticus Bar13 and Bifidobacterium longum Bar33, on the gut microbiota composition and metabolic profiles of 20 healthy subjects.ResultsThe synbiotic food did not modify the overall structure of the gut microbiome, as indicated by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The ability of the probiotic L. helveticus and B. longum strains to pass through the gastrointestinal tract was hypothesized on the basis of real-time PCR data. In spite of a stable microbiota, the intake of the synbiotic food resulted in a shift of the fecal metabolic profiles, highlighted by the Gas Chromatography Mass Spectrometry Solid Phase Micro-Extraction (GC-MS/SPME) analysis. The extent of short chain fatty acids (SCFA), ketones, carbon disulfide and methyl acetate was significantly affected by the synbiotic food consumption. Furthermore, the Canonical discriminant Analysis of Principal coordinates (CAP) of GC-MS/SPME profiles allowed a separation of the stool samples recovered before and after the consumption of the functional food.ConclusionIn this study we investigated the global impact of a dietary intervention on the gut ecology and metabolism in healthy humans. We demonstrated that the intake of a synbiotic food leads to a modulation of the gut metabolic activities with a maintenance of the gut biostructure. In particular, the significant increase of SCFA, ketones, carbon disulfide and methyl acetate following the feeding period suggests potential health promoting effects of the synbiotic food.

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Section: Discussionmentioning

confidence: 99%

Impact of a synbiotic food on the gut microbial ecology and metabolic profiles

et al. 2010

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“…The method used is a standard procedure for measuring PGE 2 production in cells, and has previously been described in detail. [27][28][29] In brief, HT29 cells were seeded out at a concentration of 3.30ϫ10 5 cells/well. At day 2, 100 mM aspirin was added to the wells to prevent activation of COX-1.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Cyclooxygenase Inhibiting Effects of Six Major Cannabinoids Isolated from Cannabis sativa

Ruhaak

Felth

Karlsson

et al. 2011

Biological & Pharmaceutical Bulletin

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123

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“…Methyl ketones are also used as food additives for providing scent and flavoring . In particular, 2‐pentanone is a phytochemical naturally produced in banana and carrot, and has been demonstrated to inhibit cyclooxygenase 2 (COX‐2), which is involved in inflammatory processes and potentially associated with colon cancer. Recently, E. coli has been engineered to produce long chain methyl ketones with 11–15 carbons by reassimilating fatty acids into fatty acyl‐coenzyme A (CoA) .…”

Section: Introductionmentioning

confidence: 99%

Metabolic engineering of 2‐pentanone synthesis in Escherichia coli

Lan¹,

Dekishima

Chuang³

et al. 2013

AIChE Journal

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Expanding the chemical diversity of microbial fermentation products enables green production of fuel, chemicals, and pharmaceuticals. In recent years, coenzyme A (CoA) dependent chain elongation, resembling the reversed β‐oxidation pathway, has attracted interest for its use in producing higher alcohols, fatty acids, and polyhydroxyalkanoate. To expand the chemical diversity of this pathway, we metabolically engineered Escherichia coli to produce 2‐pentanone, which is not a natural fermentation product of E. coli. We describe the first demonstration of 2‐pentanone synthesis in E. coli by coupling the CoA‐dependent chain elongation with the acetone production pathway. By bioprospecting for enzymes capable of efficient hydrolysis of 3‐keto‐hexanoyl‐CoA, production of 2‐pentanone increased 20 fold, reaching a titer of 240 mg/L. © 2013 American Institute of Chemical Engineers AIChE J, 59: 3167–3175, 2013

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The Flavouring Phytochemical 2-Pentanone Reduces Prostaglandin Production and COX-2 Expression in Colon Cancer Cells

Cited by 28 publications

References 46 publications

Impact of a synbiotic food on the gut microbial ecology and metabolic profiles

Impact of a synbiotic food on the gut microbial ecology and metabolic profiles

Evaluation of the Cyclooxygenase Inhibiting Effects of Six Major Cannabinoids Isolated from Cannabis sativa

Metabolic engineering of 2‐pentanone synthesis in Escherichia coli

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