The Fluidity of Phosphocholine and Maltoside Micelles and the Effect of CHAPS

Kieber, Marissa; Ono, Tomihiro; Oliver, Ryan C.; Nyenhuis, Sarah B.; Tieleman, D. Peter; Columbus, Linda

doi:10.1016/j.bpj.2019.03.019

Cited by 3 publications

(1 citation statement)

References 60 publications

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“…Due to their canonical architecture of single head and tail groups, micelles formed by these detergents are markedly more dynamic than cell membranes. 11 In addition, detergent micelles, particularly those formed by harsh detergents, can remove closely associated lipid molecules important for membrane protein structure and function during protein extraction. Therefore, it is necessary to develop novel amphiphiles able to both form micelles with reduced dynamics and retain lipid molecules essential for protein function.…”

Section: Introductionmentioning

confidence: 99%

Melamine-cored glucosides for membrane protein solubilization and stabilization: importance of water-mediated intermolecular hydrogen bonding in detergent performance

Ghani,

Kim,

Ehsan

et al. 2023

Chem. Sci.

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Section: Introductionmentioning

confidence: 99%

Melamine-cored glucosides for membrane protein solubilization and stabilization: importance of water-mediated intermolecular hydrogen bonding in detergent performance

Ghani,

Kim,

Ehsan

et al. 2023

Chem. Sci.

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Lipid bilayer strengthens the cooperative network of membrane proteins

Muhammednazaar

Yao

Guo

et al. 2023

Preprint

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Lipid bilayer provides a two-dimensional hydrophobic solvent milieu for membrane proteins in cells. Although the native bilayer is widely recognized as an optimal environment for folding and function of membrane proteins, the underlying physical basis remains elusive. Here, employing the intramembrane protease GlpG of Escherichia coli as a model, we elucidate how the bilayer stabilizes a membrane protein and engages the residue interaction network compared to the nonnative hydrophobic medium, micelles. We find that the bilayer enhances GlpG stability by promoting residue burial in the protein interior compared to micelles. Strikingly, while the cooperative residue interactions cluster into multiple distinct regions in micelles, the whole packed regions of the protein act as a single cooperative unit in the bilayer. Molecular dynamics (MD) simulation indicates that lipids less efficiently solvate GlpG than detergents. Thus, the bilayer-induced enhancement of stability and cooperativity likely stems from the dominant intraprotein interactions outcompeting the weak lipid solvation. Our findings reveal a foundational mechanism in the folding, function, and quality control of membrane proteins. The enhanced cooperativity benefits function facilitating propagation of local structural perturbation across the membrane. However, the same phenomenon can render the conformational integrity of a protein vulnerable to missense mutations causing conformational diseases.

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Utilizing Flaxseed as an Antimicrobial Alternative in Chickens: Integrative Review for Salmonella enterica and Eimeria

Weston,

Hales,

Hales

2024

CIMB

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This review provides an integrative framework for understanding flaxseed (Linum utassitissimum) as an antimicrobial alternative for poultry production. We begin by familiarizing the reader with the global legislation of antibiotics in animal husbandry; highlighting gaps and current issues for Salmonella enterica (S. enterica) and Eimeria (coccidiosis-inducing). We then discuss the natural, symbiotic characteristics of the Galliformes order (chicken-like birds) and Linum (the flaxes). The key immunological themes in this review include: (i) flaxseed’s regulation of innate and adaptive immunity in chickens, (ii) flaxseed’s ability to accelerate chicken recovery from infection with S. enterica and Eimeria, and (iii) flaxseed’s strengthening of immunity via vitamin B6 antagonism. Research indicates that whole flaxseed increases adaptive immune capacity by augmenting cecal Bacteroides and short-chain fatty acids while also attenuating the heterophil to lymphocyte ratio in chickens. Moreover, flaxseed accelerates chicken recovery from infection with Salmonella Enteritidis or Eimeria tenella; however, future work is needed to better understand (i) defatted flaxseed’s superior performance against Eimeria species and (ii) Eimeria maxima’s resilience against whole flaxseed. In the context of vitamin B6 antagonism, we propose that 15% whole flaxseed overcomes S. enterica’s insult to estrogen synthesis by sustaining the activity of phosphatidylethanolamine methyltransferase (PEMT) in liver. We also propose that 10% defatted flaxseed (as a metformin homologue) strengthens chicken immunity by safeguarding gonadal physiology and by increasing plasma thymidine bioavailability. The concepts in this review can be used as a template for conducting advanced immunological studies in poultry science.

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The Fluidity of Phosphocholine and Maltoside Micelles and the Effect of CHAPS

Cited by 3 publications

References 60 publications

Melamine-cored glucosides for membrane protein solubilization and stabilization: importance of water-mediated intermolecular hydrogen bonding in detergent performance

Melamine-cored glucosides for membrane protein solubilization and stabilization: importance of water-mediated intermolecular hydrogen bonding in detergent performance

Lipid bilayer strengthens the cooperative network of membrane proteins

Utilizing Flaxseed as an Antimicrobial Alternative in Chickens: Integrative Review for Salmonella enterica and Eimeria

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