The fluorescence lifetime and energy migration mechanism in Photosystem I of plants

Borisov, A. Yu.; Il'ina, M.D.

doi:10.1016/0005-2728(73)90182-5

Cited by 43 publications

(5 citation statements)

References 8 publications

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“…The amplitude of state transitions was evaluated by measuring the differences in fluorescence emission upon pausing cells in either state 1 or state 2 at room temperature (Fleischmann et al , 1999; Wollman, 2001) or at 77K (Allorent et al , 2013). Room temperature florescence emission from whole cells essentially comes from PSII, as the fluorescence quantum yield of PSI is extremely low (Borisov and Il’ina, 1973): changes in maximum fluorescence emission at room temperature upon induction of state 1 to state 2 transition are reported in Fig. 7A, showing a similar amplitude for wild-type and knock-down strains.…”

Section: Resultsmentioning

confidence: 98%

The function of LHCBM4/6/8 antenna proteins inChlamydomonas reinhardtii

Girolomoni

Ferrante

Berteotti

et al. 2016

EXBOTJ

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Section: Resultsmentioning

confidence: 98%

The function of LHCBM4/6/8 antenna proteins inChlamydomonas reinhardtii

Girolomoni

Ferrante

Berteotti

et al. 2016

EXBOTJ

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“…A long-lived component of fluorescence (-0.3-1 ns) is always present in the streak camera data even with single pulse excitation. The short-lived component (-50 ps) observed previously (1)(2)(3)(4)(5) should now be interpreted as due to exciton-exciton annihilation, although a short component of about this duration would be present due to photosystem I fluorescence emission (17). The exciton-exciton interpretation is favored because lifetimes determined by the streak camera for many different systems are the same indicating a possible anomaly (A. J. Campillo and S. L. Shapiro, unpublished), and because of these quantum efficiency measurements.…”

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confidence: 75%

Picosecond exciton annihilation in photosynthetic systems

Campillo¹,

Shapiro²,

Kollman³

et al. 1976

Biophysical Journal

102

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We have previously reported measurements of fluorescence lifetimes for both photosynthetic systems and chlorophyll solutions using a streak camera technique (1, 2) and picosecond excitation. Our lifetime measurements on antenna systems, however, as well as earlier results obtained using a picosecond resolution optical gate (3-5), indicate somewhat shorter decay times than measured with previous techniques (6-10). Our present experimental investigations of a possible intensity-dependent effect as the cause of these differences are prompted by the recent results of Mauzerall (11). He has shown that the quantum efficiency for the fluorescence emission from Chlorella decreases at higher pumping intensities for 7-ns duration excitation pulses, and he has interpreted the decrease in terms of a multitrap model of the photosynthetic unit and exciton-exciton collisions. In order to assess the respective roles that singlet and triplet excitons play in the kinetics of these interactions, it is desirable to repeat these measurements using a much shorter excitation pulse and under conditions comparable to the previously mentioned picosecond experiments. In this letter we report results for the quantum efficiency of Chlorella as a function of intensity, but with picosecond excitation. A single 20 ps pulse has been selected from a mode-locked laser pulse train for these measurements. Results show a drop of quantum efficiency with intensity in agreement with results of Mauzerall. Previous picosecond fluorescence lifetime measurements must be reinterpreted in view of this nonlinear optical effect.In order to investigate the possibility that the somewhat shorter lifetimes obtained by picosecond techniques for antenna systems originate from nonlinear optical effects present at high-energy densities, a different experimental arrangement than we used previously for lifetime measurements is required for quantum efficiency measurements. Previously, all the pulses in a mode-locked pulse train were allowed to excite the sample, and the fluorescence produced by one of the pulses could be examined. When making intensity-dependent quantum efficiency measurements, such a pulse train technique may lead to difficulties in interpretation. For example, pulses exciting the sample prior to the pulse chosen for investigation may populate the reaction centers, leave a residual population of antenna chlorophyll molecules in the triplet state, or otherwise change the system. Thereafter, singlets generated by the pulse to be exam-BIOPHYSICAL JOURNAL VOLUME 16 1976 93

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“…Upon various treatments and association of pigment molecules their most conservative parameter is jZ(v)dv which changes by <20% [9]. Consequently, we may simply compare those integrals for our minor fraction, i.e., the square under the B905 bleaching band upon its saturation at high excitation energies with that for B880 bulk BChl.…”

Section: Discussionmentioning

confidence: 99%