The fluorescent capillary fill device

Robinson, G.A.; Attridge, John; Deacon, J.K.; Whiteley, S.C.

doi:10.1016/0925-4005(93)85260-h

Cited by 9 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Prostate cancer is one of the most significant of cancers in males in terms of prevalence and associated mortality. 4. In the U.S.A. it is the only tumour marker recommended for screening and screening is recommended for all males from middle age.…”

Section: Adequate Accuracy and Precisionmentioning

confidence: 98%

See 1 more Smart Citation

<title>Fluorescent capillary fill device: a quantitative whole-blood assay system suited to point-of-care use</title>

et al. 1998

View full text Add to dashboard Cite

The Fluorescence Capillary Fill Device (FCFD) has previously been shown to be a candidate test vehicle for the point of care market and instrumentation suitable for commercialisation has now been developed. The FCFD instrumentation is a fluorimeter with custom optics to collect a given angular spread of light from the output edge of the device. Rigorous selection of fluorophore, light source, filters and detector (taking into account cost, availability in manufacturing quantity and the influence of inherent blood fluorescence) was undertaken when optimising the system. The instrument utilises allophycocyanin as a near-red fluorophore, an array of ultra-bright light emitting diodes and an inexpensive photomultiplier. Assay calibration is achieved by incorporating a light source movement mechanism that allows reference zones within the device to be interrogated individually. The resulting instrument has a footprint approximately 10 inches by 8 inches, requires no specialist operator training and is well suited to the point of care market. The capability of the system is illustrated by a whole blood assay for the cancer marker prostate specific antigen (PSA). This assay has a sensitivity of

show abstract

Section: Adequate Accuracy and Precisionmentioning

confidence: 98%

“…An intrinsic self-checking and calibration procedure. 4. Mechanical robustness of devices with reasonable storage conditions and shelf life.…”

Section: Adequate Accuracy and Precisionmentioning

confidence: 99%

<title>Fluorescent capillary fill device: a quantitative whole-blood assay system suited to point-of-care use</title>

et al. 1998

View full text Add to dashboard Cite

show abstract

“…For a more detailed description we refer to [6]. Other related references are [8,18,19]. In its simplest form the device consists of two glass plates separated by a narrow gap.…”

Section: Fluorescence Capillary Fill Devicesmentioning

confidence: 99%

A degenerate reaction diffusion system modelling an optical biosensor

Merino

Grinfeld

McKee

1998

Z. angew. Math. Phys.

View full text Add to dashboard Cite

We investigate qualitative properties of a global semiflow associated with a degenerate reaction diffusion system with nonlinear boundary conditions. The system models an optical biosensor. By defining a suitable Liapunov function it is possible to give a complete description of the long time behaviour of the solutions. Indeed, we are able to prove that positive solutions converge to a single spatially homogeneous equilibrium state.Mathematics Subject Classification (1991). 35K57, 35K65, 35B40.

show abstract

“…The concept of capillary immunosensors was first reported in the early 1990s. 16,17 Basically, an antibody is covalently immobilized inside the capillary and the antigen sample and other reagents are introduced sequentially using a flow device. Reports have demonstrated that a capillary immunosensor can be utilized for the analysis of heart marker proteins: myoglobin, creatine kinase mb, troponin I, and fatty acid-binding protein, 18,19 and for the measurement of paclitaxel, an anticancer drug.…”

Section: Introductionmentioning

confidence: 99%

Single-Step Sandwich Immunoreaction in a Square Glass Capillary Immobilizing Capture and Enzyme-linked Antibodies for Simplified Enzyme-linked Immunosorbent Assay

et al. 2012

View full text Add to dashboard Cite

To simplify the complicated operation steps and to minimize sample and reagent amounts for enzyme-linked immunosorbent assays (ELISA), we developed a square glass capillary immunosensor containing both covalently immobilized capture antibodies and physically adsorbed enzyme-linked antibodies. The immobilization of capture antibodies (anti-human IgG) was carried out by the treatment of 3-aminopropyltriethoxy silane, glutaraldehyde, and protein-A, followed by affinity capture of the antibody. In contrast, the enzyme-linked antibodies (alkaline phosphatase (ALP)-linked anti-human IgG) were physically adsorbed on the four corners of the capillary with the aid of polyethylene glycol (PEG) acting as a scaffold. A nanoliter volume of antigen (human IgG)-containing sample solution was introduced via capillary action. This addition resulted in the release and diffusion of ALP-linked anti-human IgG into the bulk solution. This event led to a 20-min single-step sandwich immunoreaction at the inner wall of capillary; the reaction was detected through the reaction with fluorescein diphosphate (FDP) which generated a fluorescent product, fluorescein. Using this technique, we obtained an intra-capillary precision with a coefficient of variation of 9.7%. In addition, the specificity study showed that the human IgG capillary immunosensor did not respond to rabbit IgG. Quantitative analysis was possible within the response range of 10 - 5000 ng mL(-1) anti-human IgG. This capillary immunosensor can act as a single analytical unit or can be integrated into a capillary array for multiple bioanalysis.

show abstract

The fluorescent capillary fill device

Cited by 9 publications

References 9 publications

<title>Fluorescent capillary fill device: a quantitative whole-blood assay system suited to point-of-care use</title>

<title>Fluorescent capillary fill device: a quantitative whole-blood assay system suited to point-of-care use</title>

A degenerate reaction diffusion system modelling an optical biosensor

Single-Step Sandwich Immunoreaction in a Square Glass Capillary Immobilizing Capture and Enzyme-linked Antibodies for Simplified Enzyme-linked Immunosorbent Assay

Contact Info

Product

Resources

About