“…Previous studies testing survival of Earth microorganisms in simulated martian conditions have focused on spore-forming microorganisms, particularly bacteria of the Bacillus genus, since spores are hardy forms of terrestrial life that can survive Mars-like conditions (Horneck, 1993;Nicholson et al, 2000;Riesenman and Nicholson, 2000;Schuerger et al, 2003;Link et al, 2004;Newcombe et al, 2005;Tauscher et al, 2006;Zenoff et al, 2006;Fajardo-Cavazos et al, 2010). Several studies have reported on the survival of spore-forming Bacillus species isolated from clean room facilities after exposure of microbes to specific Mars-like environmental conditions (Setlow, 2001(Setlow, , 2006Nicholson et al, 2002), but recent culture-independent analyses have shown that spore-formers are not the only types of microorganisms present in assembly and clean room facilities (Venkateswaran, 2001;La Duc et al, 2007a, 2012Moissl et al, 2007Moissl et al, , 2008Vaishampayan et al, 2010Vaishampayan et al, , 2013. Some studies have focused on testing survival of extremophilic microbes that have the metabolic capabilities to inhabit Mars (La Duc et al, 2007a;Morozova et al, 2007;Gómez et al, 2010;Bauermeister et al, 2014), while a lesser number of studies have addressed survival of non-extremophilic, non-spore-forming microbes exposed to Mars-like environmental conditions (La Duc et al, 2007b;Osman et al, 2008;Berry et al, 2010).…”