The Fng3 ING protein regulates H3 acetylation and H4 deacetylation by interacting with two distinct histone‐modifying complexes

Abstract

Summary The steady‐state level of histone acetylation is maintained by histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes. INhibitor of Growth (ING) proteins are key components of the HAT or HDAC complexes but their relationship with other components and roles in phytopathogenic fungi are not well‐characterized. Here, the FNG3 ING gene was functionally characterized in the wheat head blight fungus Fusarium graminearum. Deletion of FNG3 results in defects in fungal development and pathogenesis. Unlike other ING proteins that are specifically associated with distinct complexes, Fng3 was associated with both NuA3 HAT and FgRpd3 HDAC complexes to regulate H3 acetylation and H4 deacetylation. Whereas FgNto1 mediates the FgSas3–Fng3 interaction in the NuA3 complex, Fng3 interacted with the C‐terminal region of FgRpd3 that is present in Rpd3 orthologs from filamentous fungi but absent in yeast Rpd3. The intrinsically disordered regions in the C‐terminal tail of FgRpd3 underwent phase separation, which was important for its interaction with Fng3. Furthermore, the ING domain of Fng3 is responsible for its specificities in protein–protein interactions and functions. Taken together, Fng3 is involved in the dynamic regulation of histone acetylation by interacting with two histone modification complexes, and is important for fungal development and pathogenicity.

Stage-Specific Genetic Interaction between FgYCK1 and FgBNI4 during Vegetative Growth and Conidiation in Fusarium graminearum

“…Mating on carrot agar plates and examination for perithecium formation and ascospore ejection were assayed as described [ 71 ]. Protoplast preparation and polyethylene glycol (PEG)-mediated transformation were performed as described [ 50 , 72 ]. For transformant selection, hygromycin B (CalBiochem, La Jolla, CA, USA), geneticin (Sigma, St. Louis, MO, USA), and zeocin (Invitrogen, Carlsbad, CA, USA) were added to the final concentration of 300, 400 and 700 μg/mL, respectively, to both the bottom and top agar.…”

Combatting Fusarium head blight: advances in molecular interactions between Fusarium graminearum and wheat

“…Deletion of the histone deacetylase gene HDF1 results in a significant reduction in DON production and virulence in F. graminearum (Li et al 2011). Moreover, Fng1 and Fng3, two Inhibitor of Growth (ING) proteins associated with histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes, are important for DON production (Jiang et al 2020b;Xu et al 2022a).…”

Development and Molecular Cytogenetic Identification of Two Wheat-Aegilops geniculata Roth 7Mg Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust

“…Fusarium graminearum is a caused agent of Fusarium head blight (Fhb) [35] and produces the mycotoxin deoxynivalenol (DON) in grain, which is harmful to human and animal health [36]. It is well known that there are very few sources of parents of Fhb resistance in wheat breeding, mainly including Sumai 3 and its derivatives, Wangshuibai and Wuhan 1 [8,26].…”