The Fœtal Membranes

Bourne, Gordon

doi:10.1136/pgmj.38.438.193

Cited by 107 publications

(97 citation statements)

References 4 publications

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“…5 The reticular layer contacts the spongy layer of the amnion and forms a majority of chorion's thickness. 7 The reticular network is composed of collagens I, III, IV, V, and VI. 5 The basement membrane anchors the trophoblasts to the reticular layer with collagen IV, fibronectin, and laminin.…”

Section: Introductionmentioning

confidence: 99%

“…5 The basement membrane anchors the trophoblasts to the reticular layer with collagen IV, fibronectin, and laminin. 5,7 The trophoblast layer is the deepest layer, consisting of 2-10 layers of trophoblasts which contact with the decidua. 7 In addition to physically encasing the amniotic fluid and developing fetus, amniotic membranes play an integral biological role in fetal development and progression of pregnancy; therefore, amniotic membrane grafts harbor significant biological activity, including a number of developmental cytokines.…”

Section: Introductionmentioning

confidence: 99%

“…5,7 The trophoblast layer is the deepest layer, consisting of 2-10 layers of trophoblasts which contact with the decidua. 7 In addition to physically encasing the amniotic fluid and developing fetus, amniotic membranes play an integral biological role in fetal development and progression of pregnancy; therefore, amniotic membrane grafts harbor significant biological activity, including a number of developmental cytokines. To ensure that safe amniotic tissue allografts can be obtained while also preserving bioactivity for clinical efficacy and stability for long-term storage and off-the-shelf availability, MiMedx Group (Marietta, GA) developed a gentle cleansing and dehydration process (PURION V R process) that retains the biological activities inherent in native amniotic tissue.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Properties of dehydrated human amnion/chorion composite grafts: Implications for wound repair and soft tissue regeneration

Koob¹,

Lim²,

Massee³

et al. 2014

J Biomed Mater Res

199

160

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PURIONV R processed dehydrated human amnion/chorion membrane (dHACM; MiMedx Group, Marietta, GA) tissue products were analyzed for the effectiveness of the PURION V R process in retaining the native composition of the amniotic membrane and preserving bioactivity in the resulting products. dHACM was analyzed for extracellular matrix (ECM) composition through histological staining and for growth factor content via multiplex ELISA arrays. Bioactivity was assessed by evaluating endogenous growth factor production by human dermal fibroblasts in response to dHACM and for thermal stability by mechanical tests and in vitro cell proliferation assays. Histology of dHACM demonstrated preservation of the native amnion and chorion layers with intact, nonviable cells, collagen, proteoglycan, and elastic fibers distributed in the individual layers. An array of 36 cytokines known to regulate processes involved in inflammation and wound healing were identified in dHACM. When treated with dHACM extracts, bioactivity was demonstrated through an upregulation of basic fibroblast growth factor, granulocyte colony-stimulating factor, and placental growth factor biosynthesis, three growth factors involved in wound healing, by dermal fibroblasts in vitro. After conditioning at temperatures ranging from 278.7 to 173.5 C, dHACM retained its tensile strength and ability to promote proliferation of dermal fibroblasts in vitro. Elution experiments demonstrated a soluble fraction of growth factors that eluted from the tissue and another fraction sequestered within the matrix. The PURION V R process retains the native composition of ECM and signaling molecules and preserves bioactivity. The array of cytokines preserved in dHACM are in part responsible for its therapeutic efficacy in treating chronic wounds by orchestrating a "symphony of signals" to promote healing.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Properties of dehydrated human amnion/chorion composite grafts: Implications for wound repair and soft tissue regeneration

Koob¹,

Lim²,

Massee³

et al. 2014

J Biomed Mater Res

199

160

View full text Add to dashboard Cite

show abstract

“…The amnion is also used in reconstruction procedures (e.g. vaginoplasty) and other surgical procedures in the area of the head, neck, abdomen and pelvis [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…Placental grafts for clinical purposes are prepared from the placenta of carefully selected and examined donors. The placentas are obtained by gynecologists during cesarean section or natural labor from patients who have given their informed consent [1,2]. Human amnion may be prepared in tissue banks, as biostatic or biovital grafts, it is collected from women qualified as eligible on the basis of negative results of serological tests, and medical interviews.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Amniotic Membrane and It’s Application in the Treatment of Skin Loss and Lyell's Syndrome (Toxic Epidermal Necrolysis): Current State and New Opportunities

Klama-Baryła¹,

Łabuś²,

Kitala³

et al. 2017

J Clin Exp Dermatol Res

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Amniotic membrane is a good protection against bacterial infections, reduces losses of proteins, fluids and electrolytes, decreases pain from burns and accelerates healing. Due to its richness in nutrients and low immunogenicity, it is often used as a skin substitute. Human amnion may be prepared in tissue banks, as biostatic or biovital grafts. There are various methods of amniotic graft preparations developed to make them efficient in surgical treatment and prevent the risk of transmission of infectious diseases. This paper presents the current clinical application of human amnion in the treatment of skin losses, such as burns to the face and Lyell's syndrome (toxic epidermal necrolysis). It also describes new applications of fetal membranes for other diseases. Placental grafts are often an alternative therapy in the situations when standard treatment does not yield desirable effects. Such grafts are especially beneficial in comparison to many other bioactive therapies, including low cost, easy manipulation, capability of promoting cell migration and proliferation and stimulation of stem cell activity.

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Biomedical Potential of Human Perinatal Stem Cells

Semenov

Breymann²

2013

Perinatal Stem Cells

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The Fœtal Membranes

Cited by 107 publications

References 4 publications

Properties of dehydrated human amnion/chorion composite grafts: Implications for wound repair and soft tissue regeneration

Properties of dehydrated human amnion/chorion composite grafts: Implications for wound repair and soft tissue regeneration

Preparation of Amniotic Membrane and It’s Application in the Treatment of Skin Loss and Lyell's Syndrome (Toxic Epidermal Necrolysis): Current State and New Opportunities

Biomedical Potential of Human Perinatal Stem Cells

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