The Formaldehyde Fluorescence Method for the Histochemical Demonstration of Biogenic Monoamines a Review on the Methodology

Corrodi, H.; Jonsson, Gösta

doi:10.1177/15.2.65

Cited by 749 publications

(136 citation statements)

References 39 publications

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“…The freeze-dried specimen were exposed to formaldehyde gas of optimum humidity (+ 80°C for 60 min) and further processed for the fluorescence histochemical demonstration of biogenic monoamines according to the method of Falck and Hillarp (see Corrodi & Jonsson, 1967;Fuxe, Hokfelt, Jonsson & Ungerstedt, 1970). Serial sectioning was carried out and every tenth section (10tim) preserved, embedded in Entellan (Merck) and analysed by fluorescence microscopy.…”

Section: Fluorescence Histochemistrymentioning

confidence: 99%

Selective (+)‐amphetamine Neurotoxicity on Striatal Dopamine Nerve Terminals in the Mouse

Jönsson

Nwanze

1982

British J Pharmacology

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1 Infusion of large doses of (+)-amphetamine continuously for 7 days by means of osmotic minipumps caused a long-lasting reduction of endogenous dopamine levels, dopamine nerve terminals demonstrated histochemically and [3H]-noradrenaline uptake in vitro in the striatum of mice. 2 The effect was dose-dependent, fully developed after 4 days and selective for striatal dopamine up to a dose of (+)-amphetamine of 25 jig/h. Higher doses, which produced increased mortality, also affected dopamine levels in the olfactory tubercle as well as noradrenaline in several regions. 3 Fluorescence histochemical studies using the Falck-Hillarp technique disclosed catecholamine accumulations in the striatum after (+)-amphetamine; a sign of neurotoxic damage. No effects on the dopamine cell bodies were noted. There were also no indications of neurotoxic damage to noradrenaline or 5-hydroxytryptamine neurones induced by (+)-amphetamine. 4 Large doses of (-)-amphetamine were without effect, demonstrating that the long-lasting impairment of transmitter uptake-storage mechanism in striatal dopamine nerve terminals is selective for (+)-amphetamine.5 There was a slow gradual recovery of endogenous dopamine and [3H]-noradrenaline uptake in the striatum with time, which was almost complete 6 months after the (+)-amphetamine administration.6 The results give further evidence for the view that (+)-amphetamine in large doses can have a selective neurotoxic action on a vulnerable population of a dopamine nerve terminals in the striatum. The results suggest in addition that there is a slow regrowth and regeneration with time of damaged dopamine nerve terminals.

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Section: Fluorescence Histochemistrymentioning

confidence: 99%

Selective (+)‐amphetamine Neurotoxicity on Striatal Dopamine Nerve Terminals in the Mouse

Jönsson

Nwanze

1982

British J Pharmacology

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“…They were dried overnight in an Evans-Pearse tissue freeze-drier at a vacuum of 0.01 torr. Tissues were treated with formaldehyde vapour following the standard technique (Corrodi & Jonsson, 1967) fig. 1.…”

Section: Falck and Hillarp Fluorescence Histochemical Techniquementioning

confidence: 99%

Characteristics of the internal anal sphincter and the rectum of the vervet monkey.

Rayner¹

1979

The Journal of Physiology

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SUMMARY1. The physiology of the internal anal sphincter of the vervet monkey was investigated.2. Strips of sphincter in vitro contracted to noradrenaline and adrenaline; adrenoceptors were mainly a-excitatory. Strips of rectal circular muscle relaxed to noradrenaline and contained both inhibitory a-and fi-adrenoceptors.3. All strips contracted to acetylcholine. After hyoscine or atropine, high doses of acetylcholine relaxed all strips by stimulating intramural inhibitory neurones as relaxations were blocked by tetrodotoxin and hexamethonium. Nicotine and DMPP gave relaxations with similar characteristics.4. It was concluded that relaxations to acetylcholine, nicotine and DMPP were not adrenergic as relaxations still occurred in strips from sympathetically denervated or reserpinized animals. The block of these relaxations by propranolol and guanethidine was considered to be unrelated to their actions as adrenergic blocking drugs.5. All strips relaxed to field electrical stimulation (1-5 Hz) through stimulation of intramural inhibitory neurones as tetrodotoxin blocked these relaxations. Adrenergic blocking drugs, prior reserpinization or prior section of the hypogastric nerves did not block these responses. The relaxations were not therefore adrenergic.6. 5-Hydroxytryptamine relaxed all strips but was not the transmitter in relaxations to acetylcholine, DMPP or nicotine, nor to field electrical stimulation, as desensitization of strips to 5-HT did not alter these responses.7. The circular smooth muscle of the internal anal sphincter had a dense terminal adrenergic innervation which rapidly decreased orad.8. In vivo, hypogastric nerve stimulation relaxed the rectum but contracted the sphincter. Sacral nerve root stimulation caused an after-contraction in both rectum and sphincter. In vivo, a close arterial injection of adrenaline or noradrenaline inhibited the spontaneous contraction waves of the rectum, but contracted the sphincter. Both these responses were blocked by phentolamine. 9. It was concluded that the internal anal sphincter is a discrete high pressure zone which has excitatory cholinergic and adrenergic innervations and an inhibitory non-adrenergic innervation.

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“…In comparison with the chromaffin and so called `substitute' chromaffin reactions, FIF method possesses superiority in sensitivity and specifity to CA (Corrodi and Jonsson 1967). Since the fluorescent products of CA have their peak emission at 480 nm and those of 5-HT at 525 nm, the light emitted may appear green to yellow for CA and yellow for 5-HT when yellow secondary filters are used (strongly absorbing below 480 nm).…”

Section: Discussionmentioning

confidence: 99%

Adrenal and Extra-Adrenal Pheochromocytomas: An Ultrastructural and Formaldehyde- Induced Fluorescence Study with Catecholamine Content

Kimura

Sasano

Miura

et al. 1984

Tohoku J. Exp. Med.

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The Formaldehyde Fluorescence Method for the Histochemical Demonstration of Biogenic Monoamines a Review on the Methodology

Cited by 749 publications

References 39 publications

Selective (+)‐amphetamine Neurotoxicity on Striatal Dopamine Nerve Terminals in the Mouse

Selective (+)‐amphetamine Neurotoxicity on Striatal Dopamine Nerve Terminals in the Mouse

Characteristics of the internal anal sphincter and the rectum of the vervet monkey.

Adrenal and Extra-Adrenal Pheochromocytomas: An Ultrastructural and Formaldehyde- Induced Fluorescence Study with Catecholamine Content

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