The formation of hybrid fish derived from hybridization of Megalobrama amblycephala (♀) × Siniperca chuatsi (♂)

Wang, Yude; Yao, Jiajun; Liao, An'min; Tan, Huifang; Luo, Yaxin; Wu, Ping; Wang, Shi; Chun, Zhang; Qin, Qinbo; Tao, Min; Liu, Shaojun

doi:10.1016/j.aquaculture.2021.737547

Cited by 15 publications

(5 citation statements)

References 21 publications

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“…The growth rate is a pivotal commercial trait for aquaculture, influenced by various factors [1,8]. G. przewalskii and G. eckloni are economically vital cold-water fishes in China [15,16], closely related and inhabiting similar waters [21,22].…”

Section: Differences In the Growth Ratementioning

confidence: 99%

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Molecular Mechanisms of Growth Differences in Gymnocypris przewalskii and Gymnocypris eckloni through a Comparative Transcriptome Perspective

Zhao,

Zhou

2024

Fishes

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Genetic composition plays a crucial role in the growth rate of species, and transcriptomics provides a potent tool for studying genetic aspects of growth. We explored the growth rates and transcriptomes of the Cyprinids G. przewalskii (GP) and G. eckloni (GE). A total of 500 individuals of G. przewalskii and G. eckloni, matched in size, were separately cultured for 9 months in six cement tanks (each group with three replicates). Growth indices were measured, revealing that the growth rate of GE was greater than that of GP (p < 0.05), while there was no significant difference in survival rates (p > 0.05). Simultaneously, we conducted RNA-Seq on the muscles of both GP and GE. The results indicated a significant difference of gene expression between GP and GE, identifying 5574 differentially expressed genes (DEGs). Quantitative real-time reverse transcription–polymerase chain reaction of 10 DEGs demonstrated consistency in expression profiles with the results from the RNA-Seq analysis. The DEGs were significantly enriched in glycolysis/gluconeogenesis (ko00010), arachidonic acid formation (ko00061), arginine biosynthesis (ko00220), and the MAPK (ko04013), PI3K-Akt (ko04151), mTOR (ko04150), and TGF-β (ko04350) signal pathways, as revealed by Gene Ontology (GO) enrichment and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. This study also identified some growth-related DEGs, such as IGF2, Noggin, Decorin and others. Notably, the low expression of IGF2 may be a factor contributing to the slower growth of GP than GE.

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Section: Differences In the Growth Ratementioning

confidence: 99%

“…The growth trait is one of the most important economic traits of fish, contributing significantly to the development of the aquaculture industry [8]. The GH/IGF axis primarily regulates muscle growth [9,10].…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms of Growth Differences in Gymnocypris przewalskii and Gymnocypris eckloni through a Comparative Transcriptome Perspective

Zhao,

Zhou

2024

Fishes

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“…Crossbred animals often feature heterosis, which is the increased vitality and performance of the progeny compared to the parents [6]. Interspecific hybridization is a useful strategy to achieve heterosis and improve the phenotypes and the genotypes of the offspring.…”

Section: Introductionmentioning

confidence: 99%

“…Due to the combination of beneficial traits from both male and female parents, the hybrids exhibit heterosis in growth rate, survival, and disease resistance [7]. Current hybridization experiments on fish have mostly been conducted between different genera, subfamilies, families, or orders [6,[8][9][10]. Liu [11] obtained progenies of diploid hybrids with rapid growth rates that were derived from female red crucian carp and male common carp.…”

Section: Introductionmentioning

confidence: 99%

“…Yang [12] crossed two species of Acipenser baeri ♀and Acipenser schrenckii ♂, and their hybrid F1 had a growth advantage. Wang [6] generated live hybrids from crosses between Megalobrama amblycephala (order Cypriniformes) and Siniperca chuatsiv (order Perciformes) species, which presented an obvious growth advantage over the female parent.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Growth Superiority in a Novel Gymnocypris Hybrid, Gymnocypris przewalskii ♀ × Gymnocypris eckloni ♂

Zhao,

Zhou,

Dong

et al. 2024

Genes

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Artificial hybrid breeding can optimize parental traits to cultivate excellent hybrids with enhanced economic value. In this study, we investigated the growth performance and transcriptomes of Gymnocypris przewalskii (♀) and Gymnocypris eckloni (♂) and their F1 hybrid fishes. Hatched individuals of G. przewalskii (GP) and G. eckloni (GE) of the same size and their F1 hybrids (GH) were separately cultured for eight months in three cement tanks (n = 3). The growth indexes were measured, which showed that the growth rate of the groups was GE > GH > GP, while the survival rate was GH > GE > GP. The RNA-Seq data analysis of the muscles from the three Gymnocypris fish strains revealed that gene transcription has a significant impact on F1 hybrid fish and its parents. The differentially expressed genes (DEGs) in GH show less differences with GP, but more with GE. qRT-PCR was used to confirm the expression profiles of the chosen DEGs, and the results showed positive correlations with the RNA-seq data. KEGG enrichment results indicated that the DEGs were related to a variety of molecular functions, such as glycolysis/gluconeogenesis, arachidonic acid formation, citrate cycle, and the MAPK, PI3K-Akt, or mTOR signal pathways. Subsequent analysis indicated that there may be a significant correlation between the differential expression of IGF2 and a difference in the growth of GE and GP.

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The phenotype, sex ratio and gonadal development in triploid hybrids of rainbow trout (Oncorhynchus mykiss) ♀ and brook trout (Salvelinus fontinalis) ♂

Kuciński,

Trzeciak,

Pirtań

et al. 2025

Animal Reproduction Science

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The formation of hybrid fish derived from hybridization of Megalobrama amblycephala (♀) × Siniperca chuatsi (♂)

Cited by 15 publications

References 21 publications

Molecular Mechanisms of Growth Differences in Gymnocypris przewalskii and Gymnocypris eckloni through a Comparative Transcriptome Perspective

Molecular Mechanisms of Growth Differences in Gymnocypris przewalskii and Gymnocypris eckloni through a Comparative Transcriptome Perspective

Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Growth Superiority in a Novel Gymnocypris Hybrid, Gymnocypris przewalskii ♀ × Gymnocypris eckloni ♂

The phenotype, sex ratio and gonadal development in triploid hybrids of rainbow trout (Oncorhynchus mykiss) ♀ and brook trout (Salvelinus fontinalis) ♂

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