The formation of sphingomyelin from phosphatidylcholine in plasma membrane preparations from mouse fibroblasts

Marggraf, Wolf-Dieter; Anderer, F. A.; Kanfer, Julian N.

doi:10.1016/0005-2760(81)90028-x

Cited by 77 publications

(32 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Later on it was suggested that, in mouse SV40-transformed fibroblasts, rat liver, or 3T3-L1 fibroblasts most of the activity resided in the plasma membrane. [11][12][13] SMS activity at the Golgi was later detected also in the same mouse SV40-transformed fibroblasts and in Chinese hamster lung fibroblasts and epithelial Madin-Darby canine kidney cells. [14][15][16][17] In order to clarify the issue of subcellular localization of SM synthesis, a thorough study was performed in 1990 by Futerman and coworkers using the well-characterized protocol for subcellular fractionation of rat liver and by paying particular attention to inhibit the activity of the SM-metabolizing enzyme Figure 1.…”

Section: Localization Of Sm Synthase Activity In Cellsmentioning

confidence: 74%

Tales and Mysteries of the Enigmatic Sphingomyelin Synthase Family

Holthuis

Luberto

2010

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

In the last five years tremendous progress has been made toward the understanding of the mechanisms that govern sphingomyelin (SM) synthesis in animal cells. In line with the complexity of most biological processes, also in the case of SM biosynthesis, the more we learn the more enigmatic and finely tuned the system appears. Therefore with this review we aim first, at highlighting the most significant discoveries that advanced our knowledge and understanding of SM biosynthesis, starting from the discovery of SM to the identification of the enzymes responsible for its production; and second, at discussing old and new riddles that such discoveries pose to current investigators.

show abstract

Section: Localization Of Sm Synthase Activity In Cellsmentioning

confidence: 74%

Tales and Mysteries of the Enigmatic Sphingomyelin Synthase Family

Holthuis

Luberto

2010

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

show abstract

“…In the early studies (e.g. Marggraf et al 1981;Voelker & Kennedy 1982) the plasma membrane was proposed to be a major site of SPH biosynthesis. However during biosynthetic labeling of SPH with radioactive choline, the specifi c activity of plasma membrane SPH notably lagged behind that of SPH in a Golgi/ER fraction (Cook et al 1988).…”

Section: Sphingolipids Are Synthesized On the Luminal Aspect Of A Golgimentioning

confidence: 99%

Lipid Traffic In Animal Cells

Meer¹

1989

Annual Review of Cell and Developmental Biology

View full text Add to dashboard Cite

“…Finally, the recently reported transfer of a phosphocholine moiety from phosphatidylcholine to ceramide as a major pathway for sphingomyelin synthesis [39], should be considered here. If this reaction, which has been localized in the plasma membrane of rat liver cells [39] and fibroblasts [40] and which is possibly also present in microsomes derived from mouse liver [41], is readily reversible, cellular phosphatidylcholine might, conceivably, become labeled by liposomal choline-labeled sphingomyelin through this mechanism. However, this would be difficult to reconcile with all the evidence we produced in this and preceding papers in favor of an endocytic route of liposome uptake and of an involvement of the lysosomal system.…”

Section: Con Version Of Lysophosphatidylcholine Into Phosphatidylcholmentioning

confidence: 99%

Uptake and processing of liposomal phospholipids by Kupffer cells in vitro

Dijkstra

Galen

Regts

et al. 1985

European Journal of Biochemistry

View full text Add to dashboard Cite

We investigated the intracellular metabolic fate of choline-labeled phosphatidylcholines and sphingomyelin taken up by rat Kupffer cells in maintenance culture during interaction with large unilamellar liposomes composed of cholesterol, labeled choline-phospholipid and phosphatidylserine (molar ration 5 : 4 : 1).With both labeled compounds only small proportions of water-soluble radioactivity were found to accumulate in the cells and in the culture medium, suggesting limited phospholipid degradation. However, after a lag period of 30 min progressively increasing proportions of cell-associated liposomal phospholipid were found to be converted to cellular phospholipid, nearly all of which was phosphatidylcholine. This conversion as well as the limited release of watersoluble label from the cells was inhibited by the lysosomotropic agents ammonium chloride and chloroquine.With [Me-'4C]choline-labeled lysophosphatidylcholine, label was found to become cell-associated far in excess of an encapsulated liposomal label, [3H]inulin. Without a lag period virtually all of this was rapidly converted to phosphatidylcholine, a process which was not inhibited by the lysosomotropic agents..It is concluded that Kupffer cells, after endocytosis of liposomes, degrade the liposomal phospholipids effectively but reutilize the choline moiety for de novo synthesis of cellular phosphatidylcholine.

show abstract

The formation of sphingomyelin from phosphatidylcholine in plasma membrane preparations from mouse fibroblasts

Cited by 77 publications

References 22 publications

Tales and Mysteries of the Enigmatic Sphingomyelin Synthase Family

Tales and Mysteries of the Enigmatic Sphingomyelin Synthase Family

Lipid Traffic In Animal Cells

Uptake and processing of liposomal phospholipids by Kupffer cells in vitro

Contact Info

Product

Resources

About