The formulation optimization and properties of novel oleuropein-loaded nanocarriers

Abstract

The present study sought to encapsulate oleuropein as a nutraceutical compound in order to investigate its physical properties and stability. We extracted the phenolic compounds of virgin olive leaf by ethanol-water and acetone-water solvents. The purity of this extract was confirmed by analytical high-performance liquid chromatography using oleuropein standard. Oleuropein was encapsulated with different components (lecithin, linoleic acid, glycerol monostearate, soybean oil, and Tween 80), and the effect of their contents on oleuropein-nanostructured lipid carrier (NLC) characteristics was checked by dynamic light scattering test. Moreover, several features of the optimal nanocarrier, including zeta potential, structural, morphology, stability, as well as thermal behavior were studied. The results of optimal NLC exhibited a high zeta potential as well as supreme stability versus aggregation. Thermal study indicated that oleuropein was well embedded into NLCs. The scanning electron microscope images showed that NLC samples had many spherical particles in the form of chain structure. The stable nanocarriers did not exhibit any oleuropein leakage following their analyses for 90 days at -18, 6, and 25 °C in aqueous suspension.

Encapsulation of Oleuropein in Nanostructured Lipid Carriers: Biocompatibility and Antioxidant Efficacy in Lung Epithelial Cells

“…As summarized in Table 1, it seemed that the higher the olive oil content, the smaller the particle size, which was then confirmed by TEM micrographs (see Supplementary Materials). These results were in line with other authors, who also reported a decrease in the particle size of NLCs when the liquid lipid amount was increased [52][53][54]. Nevertheless, all formulations displayed adequate sizes between 100 and 200 nm.…”

“…In light of the characterization results, NLC-OLE50 seemed to be a promising carrier for OLE with enhanced antioxidant activity and thus we continued to study its efficacy in lung epithelial cell models. Several authors have reported micro-and nanoencapsulation methods for olive leaf extracts but to the best of our knowledge, there is a lack of studies regarding the impact that the formulation process could have on the bioactivity of this polyphenol in a biological system [22,23,52].…”

Bitterness-masking assessment of luteolin encapsulated in whey protein isolate-coated liposomes

“…The PDI generally varied from 0 to 1.0; however, an excess of 0.5 represented the wide size distribution and the presence of larger particles and aggregations. 35 As shown in Fig. 1A, the PDI value of WPI-coated liposomes ranged from 0.26 to 0.38, and the PDI increased to around 0.38 to achieve a maximum value at a WPI concentration of 20%.…”

Food-Derived Bioactive Molecules from Mediterranean Diet: Nanotechnological Approaches and Waste Valorization as Strategies to Improve Human Wellness

“…Oleuropein NLS [41][42][43][44][45] CS NPs [46,47] Hydroxytyrosol Liposomes [43,44,48] CS NPs [26,[49][50][51] PLGA-co-PAA NPs [52,53] Wine Resveratrol Zein-SHA and zein-CS NPs [54,55] mPEG-PLA NPs [56] Nuts Ellagic acid polymeric NPs [57][58][59] TPGS micelles [60] Catechin PLGA NPs [61] CS NPs [62,63] Oregano and basil…”